PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus N315
• locus tag: SA_RS12760 [old locus tag: SA2222 ]
• pan locus tag^?: SAUPAN005982000
• symbol: SA_RS12760
• pan gene symbol^?: —
• synonym:
• product: Bcr/CflA family drug resistance efflux transporter

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SA_RS12760 [old locus tag: SA2222 ]
• symbol: SA_RS12760
• product: Bcr/CflA family drug resistance efflux transporter
• replicon: chromosome
• strand: +
• coordinates: 2494320..2495531
• length: 1212
• essential: no DEG other strains

⊟Accession numbers

• Location: NC_002745 (2494320..2495531) NCBI
• BioCyc: SA_RS12760 BioCyc
• MicrobesOnline: see SA2222

⊟Phenotype

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⊟DNA sequence

• 1
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  1021
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  1201
  
  ATGCAATCAACGAAAACCAAAACGAAGCATTTTTCATTTTTATTGCTAATTACGTTAGGC
  GTCATGACCGCTTTTGGCCCACTAACTATAGATATGTACGTACCATCATTACCTAAAGTG
  CAAGGTGATTTTGGTTCTACTACATCAGAAATTCAATTAACATTATCATTCACAATGATT
  GGTCTTGCACTAGGCCAATTTATCTTTGGACCTTTATCCGATGCTTTTGGTCGCAAACGG
  ATTGCTGTATCCATTTTGATCATTTTCATTTTGGTATCAGGTTTGTCTATGTTTGTTGAT
  CAATTGCCATTATTCTTAACTTTACGATTTATTCAAGGTTTAACTGGTGGTGGCGTCATC
  GTGATTGCCAAAGCCTCTGCTGGTGATAAATTTAGTGGCAACGCACTCGCTAAATTTTTA
  GCATCTTTAATGGTAGTAAATGGCATCATCACTATTCTTGCACCATTAGCTGGTGGATTA
  GCTTTATCCGTAGCAACATGGCGTTCTATTTTCACAATTTTAACTATTGTGGCACTCATC
  ATTTTAATTGGCGTCGCTTCTCAATTACCTAAAACATCTAAAGATGAATTAAAGCAGGTG
  AATTTTAGTAGCGTCATTAAAGACTTTGGAAGTCTTTTGAAAAAACCAGCATTTATTATT
  CCAATGCTATTACAAGGTTTAACTTATGTAATGCTTTTTAGTTATTCATCTGCATCGCCA
  TTTATTACTCAAAAATTGTATAATATGACACCCCAACAATTTAGTATCATGTTTGCTGTT
  AACGGTGTAGGTTTAATCATTGTCAGTCAAGTCGTTGCTTTATTAGTAGAAAAATTACAT
  CGCCACATATTATTAATCATTTTAACTATTATACAAGTGGTGGGTGTTGCTTTAATTATC
  CTGACACTTACATTCCATTTACCACTTTGGGTCTTACTCATCGCATTCTTCTTAAATGTG
  TGTCCTGTGACGTCAATTGGACCACTTGGTTTCACAATGGCTATGGAAGAACGAACAGGT
  GGCAGTGGTAACGCATCAAGTTTACTTGGCTTATTCCAATTTATCTTAGGTGGCGCTGTT
  GCACCATTAGTTGGCTTAAAAGGCGAATTTAATACATCACCATATATGATTATTATCTTC
  ATTACAGCCATTCTATTAGTCAGTCTACAAATCATTTACTTTAAAATGATTAAAAAGCAA
  CATGTCGCATAA
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  1212
  

⊟Protein

⊟General

• locus tag: SA_RS12760 [old locus tag: SA2222 ]
• symbol: SA_RS12760
• description: Bcr/CflA family drug resistance efflux transporter
• length: 403
• theoretical pI: 10.4996
• theoretical MW: 43450.2
• GRAVY: 1.00149

⊟Function

• TIGRFAM:
  drug resistance transporter, Bcr/CflA subfamily (TIGR00710; HMM-score: 241.2)
  and 13 more
  multidrug resistance protein (TIGR00880; HMM-score: 66.8)
  Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids MFS transporter, aromatic acid:H+ symporter (AAHS) family (TIGR00895; HMM-score: 61.6)
  Cellular processes Cellular processes Toxin production and resistance drug resistance MFS transporter, drug:H+ antiporter-2 (14 Spanner) (DHA2) family (TIGR00711; HMM-score: 43.1)
  Metabolism Transport and binding proteins Other drug resistance MFS transporter, drug:H+ antiporter-2 (14 Spanner) (DHA2) family (TIGR00711; HMM-score: 43.1)
  Metabolism Transport and binding proteins Cations and iron carrying compounds H+ Antiporter protein (TIGR00900; HMM-score: 40)
  Metabolism Transport and binding proteins Unknown substrate MFS transporter, metabolite:H+ symporter (MHS) family protein (TIGR00883; HMM-score: 38.4)
  Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids D-galactonate transporter (TIGR00893; HMM-score: 33.5)
  Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids MFS transporter, sialate:H+ symporter (SHS) family (TIGR00891; HMM-score: 28.8)
  Metabolism Transport and binding proteins Cations and iron carrying compounds cation transport protein (TIGR00898; HMM-score: 26.7)
  Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids phosphoglycerate transporter family protein (TIGR00881; HMM-score: 26)
  synaptic vesicle protein SV2 (TIGR01299; HMM-score: 19.7)
  Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids sugar (Glycoside-Pentoside-Hexuronide) transporter (TIGR00792; HMM-score: 19.5)
  Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids oxalate/formate antiporter family transporter (TIGR00890; HMM-score: 4.8)
• TheSEED: see SA2222
• PFAM:
  MFS (CL0015) MFS_1; Major Facilitator Superfamily (PF07690; HMM-score: 132)
  and 3 more
  MFS_2; MFS/sugar transport protein (PF13347; HMM-score: 37.5)
  Sugar_tr; Sugar (and other) transporter (PF00083; HMM-score: 33.8)
  no clan defined DUF5305; Family of unknown function (DUF5305) (PF17231; HMM-score: 7.3)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic Membrane
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 10
  • Cellwall Score: 0
  • Extracellular Score: 0
  • Internal Helices: 12
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.338888
  • TAT(Tat/SPI): 0.007115
  • LIPO(Sec/SPII): 0.091265
• predicted transmembrane helices (TMHMM): 12

⊟Accession numbers

• GI: 447114819 NCBI
• RefSeq: WP_001192075 NCBI
• UniProt: see SA2222

⊟Protein sequence

• MQSTKTKTKHFSFLLLITLGVMTAFGPLTIDMYVPSLPKVQGDFGSTTSEIQLTLSFTMIGLALGQFIFGPLSDAFGRKRIAVSILIIFILVSGLSMFVDQLPLFLTLRFIQGLTGGGVIVIAKASAGDKFSGNALAKFLASLMVVNGIITILAPLAGGLALSVATWRSIFTILTIVALIILIGVASQLPKTSKDELKQVNFSSVIKDFGSLLKKPAFIIPMLLQGLTYVMLFSYSSASPFITQKLYNMTPQQFSIMFAVNGVGLIIVSQVVALLVEKLHRHILLIILTIIQVVGVALIILTLTFHLPLWVLLIAFFLNVCPVTSIGPLGFTMAMEERTGGSGNASSLLGLFQFILGGAVAPLVGLKGEFNTSPYMIIIFITAILLVSLQIIYFKMIKKQHVA

⊟Experimental data

• experimentally validated: data available for NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications