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PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159JSNZLGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA_RS13660 [old locus tag: SA2386 ]
  • pan locus tag?: SAUPAN006288000
  • symbol: SA_RS13660
  • pan gene symbol?: tnpC
  • synonym:
  • product: transposase

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SA_RS13660 [old locus tag: SA2386 ]
  • symbol: SA_RS13660
  • product: transposase
  • replicon: chromosome
  • strand: -
  • coordinates: 2674325..2674702
  • length: 378
  • essential: no DEG

Accession numbers[edit | edit source]

  • Location: NC_002745 (2674325..2674702) NCBI
  • BioCyc: SA_RS13660 BioCyc
  • MicrobesOnline: see SA2386

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    ATGGATAAACAAGTTAGAAATACAACAGAAATTGTACGTTTGGCGAAGCAGAAATCAAAA
    AAGACAAGGGAAAAAGTAGACAAAGCGATTTCTAAATTTTCGATTGAAGGTAAAGTTATT
    AATTTTAATTCAATAGCAAAGGAAGCTAATGTTTCTAAATCATGGCTTTATAAGGAACAC
    GATATTAGGCAAAGAATCGAATCCCTTCGTGAGCGTCAAATAACAGCAAATGTAGTCTCA
    AAACCCAAGAAAAGTTCTCGTTCGGAGGAAATCCTTATTAAAACCTTAAAAAGAAGAGTA
    ATGGAATTAGAAAAAGAAAATAAAAAATTACAGAACCAAATTCAAAAATTATATGGAGAT
    CTGTATAATAAAGAATAA
    60
    120
    180
    240
    300
    360
    378

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SA_RS13660 [old locus tag: SA2386 ]
  • symbol: SA_RS13660
  • description: transposase
  • length: 125
  • theoretical pI: 10.822
  • theoretical MW: 14804.1
  • GRAVY: -1.0384

Function[edit | edit source]

  • TIGRFAM:
    DNA 3'-phosphatase (TIGR01664; EC 3.1.3.32; HMM-score: 11.3)
  • TheSEED: see SA2386
  • PFAM:
    HTH (CL0123) DUF6262; Family of unknown function (DUF6262) (PF19776; HMM-score: 55.4)
    and 10 more
    CoA-acyltrans (CL0149) Transferase; Transferase family (PF02458; HMM-score: 18.6)
    INO80_complex (CL0850) INO80F; INO80 complex subunit F (PF24245; HMM-score: 18)
    no clan defined HALZ; Homeobox associated leucine zipper (PF02183; HMM-score: 16.8)
    HTH (CL0123) LacI; Bacterial regulatory proteins, lacI family (PF00356; HMM-score: 16.2)
    bZIP (CL0018) bZIP_2; Basic region leucine zipper (PF07716; HMM-score: 15.5)
    TetR_C (CL0174) TetR_C_38; Tetracyclin repressor-like, C-terminal domain (PF19352; HMM-score: 13.1)
    FtsL (CL0225) ZapB; Cell division protein ZapB (PF06005; HMM-score: 12.8)
    P-loop_NTPase (CL0023) AAA_11; AAA domain (PF13086; HMM-score: 8.8)
    no clan defined DUF4337; Domain of unknown function (DUF4337) (PF14235; HMM-score: 8.3)
    FtsL (CL0225) DivIC; Septum formation initiator (PF04977; HMM-score: 7.7)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.9889
    • Cytoplasmic Membrane Score: 0.0072
    • Cell wall & surface Score: 0.0018
    • Extracellular Score: 0.0021
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.007501
    • TAT(Tat/SPI): 0.000595
    • LIPO(Sec/SPII): 0.001342
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MDKQVRNTTEIVRLAKQKSKKTREKVDKAISKFSIEGKVINFNSIAKEANVSKSWLYKEHDIRQRIESLRERQITANVVSKPKKSSRSEEILIKTLKRRVMELEKENKKLQNQIQKLYGDLYNKE

Experimental data[edit | edit source]

  • experimentally validated:
  • protein localization:
  • quantitative data / protein copy number per cell:
  • interaction partners:

Expression & Regulation[edit | edit source]

Operon[edit | edit source]

Regulation[edit | edit source]

  • regulator:

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]