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NCBI: 02-MAR-2017

Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA_RS14165 [old locus tag: SA2475 ]
  • pan locus tag?: SAUPAN006439000
  • symbol: SA_RS14165
  • pan gene symbol?:
  • synonym:
  • product: energy-coupling factor transporter transmembrane protein EcfT

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SA_RS14165 [old locus tag: SA2475 ]
  • symbol: SA_RS14165
  • product: energy-coupling factor transporter transmembrane protein EcfT
  • replicon: chromosome
  • strand: -
  • coordinates: 2786397..2787230
  • length: 834
  • essential: no DEG other strains

Accession numbers[edit | edit source]

  • Location: NC_002745 (2786397..2787230) NCBI
  • BioCyc: SA_RS14165 BioCyc
  • MicrobesOnline: see SA2475

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    ATGAATCAATATAATACTATAGGTTTTCACCCGGGAAATAGTCGTATTCATCAATTAAAT
    GCGACTGTTAAACTTTTATTCTTATTAGTTGTTTCTATTTCTGCAATGGTGACTTATGAC
    ACAAGATATTTAATTTTAATTAGTGCTTCATCTATTTTATTGTTCAAATATGCTCATATT
    GAATGGAAACAAGTTCGATTTGTTGTTAAATTCATTCTGTTTTTCACAATATTAAATATT
    ATTGCCGTATACATATTTGACCCTGAATATGGTGTGAAGATTTATAATCAGCGTACAGAG
    TTAGTCAATGGTATTGGTCGATTTACGCTAACATCACAGGAATTATTCTATCTTTTTAAT
    CTAATATTAAAATATATTAGTACAGTTCCTTTAGCGTTAATATTTTTATTCACAACGAAT
    CCGAGTCATTTTGCTGCAAGTTTAAATCAGCTAGGTGTGAATTATAAAATCAGTTACGCA
    GTCTCACTAGCATTAAGGTATATTCCAGATATTCAAGAAACATATTTTAATATTTCACAA
    GCGCAACAAGCAAGAGGATATGATAATTCGAAAAAAGCGAAATTTACTAGCCGTGTTAAA
    GGTATCAAACGCATTGTGTTACCTTTAATATTTTCTAGTATCGAAAGAATTGACACTATT
    AGTACTGCTATGGAGTTAAGACAATTCGGACAGTATAAAAGGAGAACCTGGTACGTCAAA
    AAACAATTAAAAAAAGATGATTATGTTGTTTTGTGTTTGACGTTAATACTTCTGATGTTA
    GTAGTTACATTATTCTTTTTAAATAATAGTCGATATTTCAACCCGTGGCATTAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    834

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SA_RS14165 [old locus tag: SA2475 ]
  • symbol: SA_RS14165
  • description: energy-coupling factor transporter transmembrane protein EcfT
  • length: 277
  • theoretical pI: 10.2899
  • theoretical MW: 32371.9
  • GRAVY: 0.275812

Function[edit | edit source]

  • TIGRFAM:
    Metabolism Transport and binding proteins Cations and iron carrying compounds cobalt ECF transporter T component CbiQ (TIGR02454; HMM-score: 73.5)
  • TheSEED: see SA2475
  • PFAM:
    no clan defined CbiQ; Cobalt transport protein (PF02361; HMM-score: 106.6)
    and 1 more
    ABC-2 (CL0181) PDR_CDR; CDR ABC transporter (PF06422; HMM-score: 11.3)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 3
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.014217
    • TAT(Tat/SPI): 0.000355
    • LIPO(Sec/SPII): 0.009853
  • predicted transmembrane helices (TMHMM): 5

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MNQYNTIGFHPGNSRIHQLNATVKLLFLLVVSISAMVTYDTRYLILISASSILLFKYAHIEWKQVRFVVKFILFFTILNIIAVYIFDPEYGVKIYNQRTELVNGIGRFTLTSQELFYLFNLILKYISTVPLALIFLFTTNPSHFAASLNQLGVNYKISYAVSLALRYIPDIQETYFNISQAQQARGYDNSKKAKFTSRVKGIKRIVLPLIFSSIERIDTISTAMELRQFGQYKRRTWYVKKQLKKDDYVVLCLTLILLMLVVTLFFLNNSRYFNPWH

Experimental data[edit | edit source]

  • experimentally validated: data available for COL
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

Expression & Regulation[edit | edit source]

Operon[edit | edit source]

Regulation[edit | edit source]

  • regulator:

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]