From AureoWiki
Jump to navigation Jump to search

NCBI: 02-MAR-2017

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA_RS14180 [old locus tag: SA2478 ]
  • pan locus tag?: SAUPAN006442000
  • symbol: SA_RS14180
  • pan gene symbol?: —
  • synonym:
  • product: DNA-directed RNA polymerase subunit delta

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SA_RS14180 [old locus tag: SA2478 ]
  • symbol: SA_RS14180
  • product: DNA-directed RNA polymerase subunit delta
  • replicon: chromosome
  • strand: -
  • coordinates: 2789575..2790423
  • length: 849
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

  • Location: NC_002745 (2789575..2790423) NCBI
  • BioCyc: SA_RS14180 BioCyc
  • MicrobesOnline: see SA2478

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    ATGGGAAATTATTTGGTTTTGCAATCAGATTTTGGACTTAGTGATGGCGCTGTAAGTGCA
    ATGTATGGAGTTGCATATACTGTAAGTGATGATATACGTGTCGAAAACTTAACGCATGAT
    ATACCACCGTATGACATTTGGGTAGCGTCATATCGTTTATATCAAACTGTAAAATACTGG
    CCTAAAGGCACAGTTTTTGTATCAGTGGTAGACCCGGGTGTAGGTAGTGATAGGCGGAGC
    ATTGCTTGCTTAACATATTCAGGTCATTACATTATTACACCTGATAATGGTTCTTTAAGT
    CATATTAAACATTACGAAGGTATTAAAAAAGTTATCGAAATTGATGAAGTGAAAAGCCGA
    TTGCCTCATTCGGAAGAAAGTCATACTTTTCATGGTAGAGATGTTTATGCATACAATGGT
    GCGAGATTAGCTGCAAATAAGATAGCGTTTGAACGTCTCGGTCAGGCAATTAATGTTGAT
    AGTATTGAGGCATTAGAAATAAGAGAAGCGACAAAAAATGAGGATTCTGTTACAGGAAGC
    ATTGATATTTTAGATATCAGATTTGGGTCATTATGGACAAATATTCCATTGGCATTCTTA
    AAATCCTTGGAAATTGTTCATGGTAATAATTTGGTGGTAACAATTTATCATCAAGATAAG
    AAAGTCTATCAAAATATTATTAAATTTGCGCGATCGTTTGCAGATGTTAATGTGGGTGAA
    CCTCTTGTGTATGTTAACTCGCTAGTAAATATTGGTGTAGCAGTCAATCAAGACTCATTT
    TCAGATTTATATCATATTGGTACTGGAAATGATTGGACGATTCATTTATCTAAAGCACCA
    TCAATTTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    849

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SA_RS14180 [old locus tag: SA2478 ]
  • symbol: SA_RS14180
  • description: DNA-directed RNA polymerase subunit delta
  • length: 282
  • theoretical pI: 5.93783
  • theoretical MW: 31411.2
  • GRAVY: -0.0904255

⊟Function[edit | edit source]

  • TIGRFAM:
    Cellular processes Cellular processes Biosynthesis of natural products adenosyl-fluoride synthase (TIGR04507; EC 2.5.1.63; HMM-score: 78.7)
    and 1 more
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Pyridine nucleotides nicotinamide-nucleotide adenylyltransferase (TIGR01527; EC 2.7.7.1; HMM-score: 15.2)
  • TheSEED: see SA2478
  • PFAM:
    no clan defined SAM_adeno_trans; S-adenosyl-l-methionine hydroxide adenosyltransferase (PF01887; HMM-score: 232.4)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.01588
    • TAT(Tat/SPI): 0.002943
    • LIPO(Sec/SPII): 0.00374
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MGNYLVLQSDFGLSDGAVSAMYGVAYTVSDDIRVENLTHDIPPYDIWVASYRLYQTVKYWPKGTVFVSVVDPGVGSDRRSIACLTYSGHYIITPDNGSLSHIKHYEGIKKVIEIDEVKSRLPHSEESHTFHGRDVYAYNGARLAANKIAFERLGQAINVDSIEALEIREATKNEDSVTGSIDILDIRFGSLWTNIPLAFLKSLEIVHGNNLVVTIYHQDKKVYQNIIKFARSFADVNVGEPLVYVNSLVNIGVAVNQDSFSDLYHIGTGNDWTIHLSKAPSI

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

Retrieved from "http://aureowiki.med.uni-greifswald.de/index.php?title=SA_RS14180&oldid=86838"