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NCBI: 06-JUL-2013

Summary[edit | edit source]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_1085 [new locus tag: NWMN_RS06155 ]
  • pan locus tag?: SAUPAN003442000
  • symbol: NWMN_1085
  • pan gene symbol?:
  • synonym:
  • product: hypothetical protein

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: NWMN_1085 [new locus tag: NWMN_RS06155 ]
  • symbol: NWMN_1085
  • product: hypothetical protein
  • replicon: chromosome
  • strand: +
  • coordinates: 1188857..1189552
  • length: 696
  • essential: unknown other strains

Accession numbers[edit | edit source]

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    ATGGGGAAATTGGGATATAAAAATATTTTGATAGACTTTGATGATACAATTGTTGATTTT
    TATGATGCAGAAGAATGGGCGTTTCACTATATGGCGAATGTTTTTAATCATAAAGCAACA
    AAGGATGATTTTTTAACATTTAAAAAAATCAATCACCAACATTGGGAAGCTTTTCAACAA
    AATAAATTAACGAAGTCTGAAGTATTATCAGAACGATTTGTGAATTACTTCAAACATCAT
    CAAATGGAAGTTGATGGGCATCGTGCAGATGTGTTATTTAGAAATGGATTAGCAGAAGCT
    AAAGTTAAATACTTTGATCAAACATTAGAAACAATTGTCGAATTATCGAAAAGACATGAT
    TTATATATTGTTACTAATGGTGTAACCGAAACGCAAAAGAGAAGGTTAAATCAGACGCCG
    TTGCATAAATATATTAAAAAGATATTTATATCTGAAGAAACAGGATATCAAAAACCTAAT
    CCGGAATTTTTTAATTATGTTTTTAATGATATTGGTGAGGATGAAAGACAGCACTCGATT
    ATAGTTGGAGATTCTTTAACATCTGACATTCTAGGTGGAATCAATGCGGGTATAGCTACT
    TGCTGGTTTAATTTTAGAGGATTTGATCATAATCCAGGAATTATACCTGATTATGAAATT
    AATTCATGGAAACAACTAAATGATATTGTACGTTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    696

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: NWMN_1085 [new locus tag: NWMN_RS06155 ]
  • symbol: NWMN_1085
  • description: hypothetical protein
  • length: 231
  • theoretical pI: 6.28512
  • theoretical MW: 27205.4
  • GRAVY: -0.5671

Function[edit | edit source]

  • TIGRFAM:
    noncanonical pyrimidine nucleotidase, YjjG family (TIGR02254; EC 3.1.3.5; HMM-score: 200.1)
    and 17 more
    HAD hydrolase, TIGR02253 family (TIGR02253; HMM-score: 94.7)
    Unknown function Enzymes of unknown specificity HAD hydrolase, family IA, variant 1 (TIGR01549; HMM-score: 54.5)
    haloacid dehalogenase, type II (TIGR01428; EC 3.8.1.2; HMM-score: 41)
    HAD hydrolase, REG-2-like, family IA (TIGR02252; HMM-score: 41)
    Unknown function Enzymes of unknown specificity HAD hydrolase, family IIA (TIGR01460; HMM-score: 37.8)
    Unknown function Enzymes of unknown specificity HAD hydrolase, family IIIA (TIGR01662; HMM-score: 33.7)
    HAD hydrolase, TIGR01459 family (TIGR01459; HMM-score: 33.2)
    Unknown function Enzymes of unknown specificity HAD hydrolase, family IA, variant 3 (TIGR01509; HMM-score: 30.3)
    HAD phosphatase, family IIIA (TIGR01668; EC 3.1.3.-; HMM-score: 26.9)
    Metabolism Energy metabolism Sugars phosphoglycolate phosphatase, bacterial (TIGR01449; EC 3.1.3.18; HMM-score: 22.9)
    epoxide hydrolase N-terminal domain-like phosphatase (TIGR02247; HMM-score: 22.4)
    histidinol-phosphate phosphatase domain (TIGR01656; HMM-score: 21.2)
    beta-phosphoglucomutase family hydrolase (TIGR02009; HMM-score: 19.7)
    Unknown function Enzymes of unknown specificity HAD hydrolase, TIGR01457 family (TIGR01457; HMM-score: 19.5)
    Unknown function Enzymes of unknown specificity HAD hydrolase, TIGR01458 family (TIGR01458; HMM-score: 13.8)
    Metabolism Energy metabolism Biosynthesis and degradation of polysaccharides beta-phosphoglucomutase (TIGR01990; EC 5.4.2.6; HMM-score: 13.8)
    HAD hydrolase, family IA, variant 2 (TIGR01493; HMM-score: 12.9)
  • TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
  • PFAM:
    HAD (CL0137) HAD_2; haloacid dehalogenase-like hydrolase (PF13419; HMM-score: 78.8)
    and 3 more
    Hydrolase_like; HAD-hyrolase-like (PF13242; HMM-score: 36.3)
    Hydrolase; haloacid dehalogenase-like hydrolase (PF00702; HMM-score: 36.1)
    HAD; haloacid dehalogenase-like hydrolase (PF12710; HMM-score: 14.7)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.002276
    • TAT(Tat/SPI): 0.000119
    • LIPO(Sec/SPII): 0.000249
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MGKLGYKNILIDFDDTIVDFYDAEEWAFHYMANVFNHKATKDDFLTFKKINHQHWEAFQQNKLTKSEVLSERFVNYFKHHQMEVDGHRADVLFRNGLAEAKVKYFDQTLETIVELSKRHDLYIVTNGVTETQKRRLNQTPLHKYIKKIFISEETGYQKPNPEFFNYVFNDIGEDERQHSIIVGDSLTSDILGGINAGIATCWFNFRGFDHNPGIIPDYEINSWKQLNDIVR

Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

Expression & Regulation[edit | edit source]

Operon[edit | edit source]

Regulation[edit | edit source]

  • regulator:

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]