PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 06-JUL-2013

⊟Summary

• organism: Staphylococcus aureus Newman
• locus tag: NWMN_1085 [new locus tag: NWMN_RS06155 ]
• pan locus tag^?: SAUPAN003442000
• symbol: NWMN_1085
• pan gene symbol^?: —
• synonym:
• product: hypothetical protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: NWMN_1085 [new locus tag: NWMN_RS06155 ]
• symbol: NWMN_1085
• product: hypothetical protein
• replicon: chromosome
• strand: +
• coordinates: 1188857..1189552
• length: 696
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 5330668 NCBI
• RefSeq: YP_001332119 NCBI
• BioCyc:
• MicrobesOnline: 3706637 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

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  ATGGGGAAATTGGGATATAAAAATATTTTGATAGACTTTGATGATACAATTGTTGATTTT
  TATGATGCAGAAGAATGGGCGTTTCACTATATGGCGAATGTTTTTAATCATAAAGCAACA
  AAGGATGATTTTTTAACATTTAAAAAAATCAATCACCAACATTGGGAAGCTTTTCAACAA
  AATAAATTAACGAAGTCTGAAGTATTATCAGAACGATTTGTGAATTACTTCAAACATCAT
  CAAATGGAAGTTGATGGGCATCGTGCAGATGTGTTATTTAGAAATGGATTAGCAGAAGCT
  AAAGTTAAATACTTTGATCAAACATTAGAAACAATTGTCGAATTATCGAAAAGACATGAT
  TTATATATTGTTACTAATGGTGTAACCGAAACGCAAAAGAGAAGGTTAAATCAGACGCCG
  TTGCATAAATATATTAAAAAGATATTTATATCTGAAGAAACAGGATATCAAAAACCTAAT
  CCGGAATTTTTTAATTATGTTTTTAATGATATTGGTGAGGATGAAAGACAGCACTCGATT
  ATAGTTGGAGATTCTTTAACATCTGACATTCTAGGTGGAATCAATGCGGGTATAGCTACT
  TGCTGGTTTAATTTTAGAGGATTTGATCATAATCCAGGAATTATACCTGATTATGAAATT
  AATTCATGGAAACAACTAAATGATATTGTACGTTAA
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⊟Protein

⊟General

• locus tag: NWMN_1085 [new locus tag: NWMN_RS06155 ]
• symbol: NWMN_1085
• description: hypothetical protein
• length: 231
• theoretical pI: 6.28512
• theoretical MW: 27205.4
• GRAVY: -0.5671

⊟Function

• TIGRFAM:
  noncanonical pyrimidine nucleotidase, YjjG family (TIGR02254; EC 3.1.3.5; HMM-score: 200.1)
  and 17 more
  HAD hydrolase, TIGR02253 family (TIGR02253; HMM-score: 94.7)
  Unknown function Enzymes of unknown specificity HAD hydrolase, family IA, variant 1 (TIGR01549; HMM-score: 54.5)
  haloacid dehalogenase, type II (TIGR01428; EC 3.8.1.2; HMM-score: 41)
  HAD hydrolase, REG-2-like, family IA (TIGR02252; HMM-score: 41)
  Unknown function Enzymes of unknown specificity HAD hydrolase, family IIA (TIGR01460; HMM-score: 37.8)
  Unknown function Enzymes of unknown specificity HAD hydrolase, family IIIA (TIGR01662; HMM-score: 33.7)
  HAD hydrolase, TIGR01459 family (TIGR01459; HMM-score: 33.2)
  Unknown function Enzymes of unknown specificity HAD hydrolase, family IA, variant 3 (TIGR01509; HMM-score: 30.3)
  HAD phosphatase, family IIIA (TIGR01668; EC 3.1.3.-; HMM-score: 26.9)
  Metabolism Energy metabolism Sugars phosphoglycolate phosphatase, bacterial (TIGR01449; EC 3.1.3.18; HMM-score: 22.9)
  epoxide hydrolase N-terminal domain-like phosphatase (TIGR02247; HMM-score: 22.4)
  histidinol-phosphate phosphatase domain (TIGR01656; HMM-score: 21.2)
  beta-phosphoglucomutase family hydrolase (TIGR02009; HMM-score: 19.7)
  Unknown function Enzymes of unknown specificity HAD hydrolase, TIGR01457 family (TIGR01457; HMM-score: 19.5)
  Unknown function Enzymes of unknown specificity HAD hydrolase, TIGR01458 family (TIGR01458; HMM-score: 13.8)
  Metabolism Energy metabolism Biosynthesis and degradation of polysaccharides beta-phosphoglucomutase (TIGR01990; EC 5.4.2.6; HMM-score: 13.8)
  HAD hydrolase, family IA, variant 2 (TIGR01493; HMM-score: 12.9)
• TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
• PFAM:
  HAD (CL0137) HAD_2; haloacid dehalogenase-like hydrolase (PF13419; HMM-score: 78.8)
  and 3 more
  Hydrolase_like; HAD-hyrolase-like (PF13242; HMM-score: 36.3)
  Hydrolase; haloacid dehalogenase-like hydrolase (PF00702; HMM-score: 36.1)
  HAD; haloacid dehalogenase-like hydrolase (PF12710; HMM-score: 14.7)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: -1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.002276
  • TAT(Tat/SPI): 0.000119
  • LIPO(Sec/SPII): 0.000249
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 151221297 NCBI
• RefSeq: YP_001332119 NCBI
• UniProt: A0A0H3K863 UniProt

⊟Protein sequence

• MGKLGYKNILIDFDDTIVDFYDAEEWAFHYMANVFNHKATKDDFLTFKKINHQHWEAFQQNKLTKSEVLSERFVNYFKHHQMEVDGHRADVLFRNGLAEAKVKYFDQTLETIVELSKRHDLYIVTNGVTETQKRRLNQTPLHKYIKKIFISEETGYQKPNPEFFNYVFNDIGEDERQHSIIVGDSLTSDILGGINAGIATCWFNFRGFDHNPGIIPDYEINSWKQLNDIVR

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: no polycistronic organisation predicted

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications