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NCBI: 06-JUL-2013

Summary[edit | edit source]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_2013 [new locus tag: NWMN_RS11640 ]
  • pan locus tag?: SAUPAN005400000
  • symbol: atpB
  • pan gene symbol?: atpB
  • synonym:
  • product: F0F1 ATP synthase subunit A

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: NWMN_2013 [new locus tag: NWMN_RS11640 ]
  • symbol: atpB
  • product: F0F1 ATP synthase subunit A
  • replicon: chromosome
  • strand: -
  • coordinates: 2230896..2231624
  • length: 729
  • essential: unknown other strains

Accession numbers[edit | edit source]

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    ATGGATCACAAATCCCCGCTCGTGAGTTGGAATTTATTCGGTTTTGATATCGTTTTCAAT
    TTATCAAGTATATTGATGATACTTGTTACGGCGTTTCTTGTTTTTCTACTTGCTATCATT
    TGTACGCGTAATTTGAAAAAAAGACCAACTGGCAAACAAAATTTCGTTGAATGGATTTTT
    GATTTCGTGAGGGGAATCATTGAAGGTAACATGGCTTGGAAAAAAGGTGGTCAATTCCAC
    TTCTTAGCAGTAACGCTGATTCTGTACATTTTTATAGCTAATATGTTAGGTCTTCCGTTT
    TCTATAGTAACGAAAGATCACACATTGTGGTGGAAATCACCGACAGCTGATGCAACAGTG
    ACTTTAACGTTGTCTACAACGATAATACTGTTAACTCACTTTTATGGAATTAAAATGCGT
    GGTACGAAACAATATCTTAAAGGTTATGTACAGCCGTTTTGGCCATTGGCAATTATTAAT
    GTTTTTGAAGAGTTCACTTCAACATTAACGCTTGGTCTGCGTTTGTACGGTAACATATTT
    GCAGGTGAGATACTATTAACATTACTTGCTGGCTTATTCTTTAACGAACCAGCATGGGGT
    TGGATTATTAGTATCCCAGGATTAATTGTTTGGCAAGCATTTTCAATATTTGTAGGAACA
    ATCCAAGCATATATCTTTATTATGCTTTCGATGGTTTATATGTCACATAAAGTGGCAGAT
    GAACACTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    729

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: NWMN_2013 [new locus tag: NWMN_RS11640 ]
  • symbol: AtpB
  • description: F0F1 ATP synthase subunit A
  • length: 242
  • theoretical pI: 9.55099
  • theoretical MW: 27629.7
  • GRAVY: 0.679339

Function[edit | edit source]

  • reaction:
    EC 3.6.3.14?  ExPASy
    H+-transporting two-sector ATPase ATP + H2O + H+(In) = ADP + phosphate + H+(Out)
  • TIGRFAM:
    Metabolism Energy metabolism ATP-proton motive force interconversion ATP synthase F0, A subunit (TIGR01131; EC 3.6.3.14; HMM-score: 147.4)
    and 1 more
    alternate F1F0 ATPase, F0 subunit A (TIGR03306; EC 3.6.3.-; HMM-score: 77.1)
  • TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
  • PFAM:
    no clan defined ATP-synt_A; ATP synthase A chain (PF00119; HMM-score: 189.5)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 5
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.002082
    • TAT(Tat/SPI): 0.000436
    • LIPO(Sec/SPII): 0.017046
  • predicted transmembrane helices (TMHMM): 5

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MDHKSPLVSWNLFGFDIVFNLSSILMILVTAFLVFLLAIICTRNLKKRPTGKQNFVEWIFDFVRGIIEGNMAWKKGGQFHFLAVTLILYIFIANMLGLPFSIVTKDHTLWWKSPTADATVTLTLSTTIILLTHFYGIKMRGTKQYLKGYVQPFWPLAIINVFEEFTSTLTLGLRLYGNIFAGEILLTLLAGLFFNEPAWGWIISIPGLIVWQAFSIFVGTIQAYIFIMLSMVYMSHKVADEH

Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

Expression & Regulation[edit | edit source]

Regulation[edit | edit source]

  • regulator:

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]