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NCBI: 02-MAR-2017

Summary[edit | edit source]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_RS14610 [old locus tag: NWMN_2543 ]
  • pan locus tag?: SAUPAN006375000
  • symbol: NWMN_RS14610
  • pan gene symbol?:
  • synonym:
  • product: N-acetylmuramoyl-L-alanine amidase

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: NWMN_RS14610 [old locus tag: NWMN_2543 ]
  • symbol: NWMN_RS14610
  • product: N-acetylmuramoyl-L-alanine amidase
  • replicon: chromosome
  • strand: +
  • coordinates: 2801862..2803721
  • length: 1860
  • essential: unknown other strains

Accession numbers[edit | edit source]

  • Location: NC_009641 (2801862..2803721) NCBI
  • BioCyc:
  • MicrobesOnline: see NWMN_2543

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
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    1021
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    1141
    1201
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    1321
    1381
    1441
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    1621
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    1741
    1801
    ATGCCTAAAAATAAAATTTTAATTTATTTGCTATCAACTACGCTCGTATTACCTACTTTA
    GTTTCACCTACCGCTTATGCTGATACACCTCAAAAAGATACTACAGCTAAGACAACATCT
    CATGATTCAAAAAAATCTAATGACGATGAAACTTCTAAGGATACTACAAGTAAAGATATT
    GATAAAGCAGACAAAAATAATACAAGTAACCAAGACAATAACGACAAAAAATTTAAAACT
    ATAGACGACAGCACTTCAGACTCTAACAATATCATTGATTTTATTTATAAGAATTTACCA
    CAAACCAATATAAACCAATTGCTAACCAAAAATAAATACGATGATAATTACTCATTAACA
    ACTTTAATCCAAAACTTATTCAATTTAAATTCGGATATTTCTGATTACGAACAACCTCGT
    AATGGCGAAAAGTCAACAAATGATTCGAATAAAAACAGTGACAATAGCATCAAAAATGAC
    ACTGATACGCAATCATCTAAACAAGATAAAGCAGACAATCAAAAAGCACCTAAATCAAAC
    AATACAAAACCAAGTACATCTAATAAGCAACCAAATTCGCCAAAGCCAACACAACCTAAT
    CAATCAAATAGTCAACCAGCAAGTGACGATAAAGCAAATCAAAAATCTTCATCGAAAGAT
    AATCAATCAATGTCAGATTCGGCTTTAGACTCTATTTTGGATCAATACAGTGAAGATGCA
    AAGAAAACACAAAAAGATTATGCATCTCAATCTAAAAAAGACAAAAATGAAAAATCTAAT
    ACAAAGAATCCACAGTTACCAACACAAGATGAATTGAAACATAAATCTAAACCTGCTCAA
    TCATTCAATAACGATGTTAATCAAAAGGATACACGTGCAACATCATTATTCGAAACAGAT
    CCTAGTATATCTAACAATGATGATAGCGGACAATTTAACGTTGTTGACTCAAAAGATACA
    CGTCAATTTGTCAAATCAATTGCTAAAGATGCACATCGCATTGGTCAAGATAACGATATT
    TATGCGTCTGTCATGATTGCCCAAGCAATCTTAGAATCTGACTCAGGTCGTAGTGCTTTA
    GCTAAGTCACCAAACCATAATTTATTCGGTATCAAAGGTGCTTTTGAAGGGAATTCTGTT
    CCTTTTAACACATTAGAAGCTGATGGTAATCAATTGTATAGTATTAATGCTGGATTCCGA
    AAATATCCAAGCACGAAAGAATCACTAAAAGATTACTCTGACCTTATTAAAAATGGTATT
    GATGGCAATCGAACAATTTATAAACCAACATGGAAATCGGAAGCCGATTCTTATAAAGAT
    GCAACATCACACTTATCTAAAACATATGCTACAGATCCAAACTATGCTAAGAAATTAAAC
    AGTATTATTAAACACTATCAATTAACTCAGTTTGACGATGAACGCATGCCAGATTTAGAT
    AAATATGAACGTTCTATCAAGGATTATGATGATTCATCAGATGAATTCAAACCTTTCCGT
    GAGGTATCTGATAGTATGCCATATCCACATGGCCAATGTACTTGGTACGTATATAACCGT
    ATGAAACAATTTGGTACATCTATCTCAGGTGATTTAGGTGATGCACATAATTGGAATAAT
    CGAGCTCAATACCGTGATTATCAAGTAAGTCATACACCAAAACGTCATGCTGCTGTTGTA
    TTTGAGGCTGGACAATTTGGTGCAGATCAACATTACGGTCATGTAGCATTTGTTGAAAAA
    GTTAACAGTGATGGTTCTATCGTTATTTCAGAATCCAATGTTAAAGGATTAGGTATCATT
    TCTCATAGAACTATCAACGCAGCTGCCGCTGAAGAATTATCATATATTACAGGTAAATAA
    60
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    1860

Protein[edit | edit source]

Protein Data Bank: 5T1Q

General[edit | edit source]

  • locus tag: NWMN_RS14610 [old locus tag: NWMN_2543 ]
  • symbol: NWMN_RS14610
  • description: N-acetylmuramoyl-L-alanine amidase
  • length: 619
  • theoretical pI: 6.34359
  • theoretical MW: 69252.8
  • GRAVY: -1.13279

Function[edit | edit source]

  • TIGRFAM:
    flagellar rod assembly protein/muramidase FlgJ (TIGR02541; HMM-score: 81.9)
    and 1 more
    pentapeptide MXKDX repeat protein (TIGR02953; HMM-score: 15.7)
  • TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
  • PFAM:
    Lysozyme (CL0037) Glucosaminidase; Mannosyl-glycoprotein endo-beta-N-acetylglucosaminidase (PF01832; HMM-score: 75.2)
    Peptidase_CA (CL0125) CHAP; CHAP domain (PF05257; HMM-score: 66.7)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: Extracellular
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 0.09
    • Cellwall Score: 0.18
    • Extracellular Score: 9.73
    • Internal Helix: 1
  • LocateP:
  • SignalP: Signal peptide SP(Sec/SPI) length 27 aa
    • SP(Sec/SPI): 0.99354
    • TAT(Tat/SPI): 0.004521
    • LIPO(Sec/SPII): 0.001352
    • Cleavage Site: CS pos: 27-28. AYA-DT. Pr: 0.9885
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MPKNKILIYLLSTTLVLPTLVSPTAYADTPQKDTTAKTTSHDSKKSNDDETSKDTTSKDIDKADKNNTSNQDNNDKKFKTIDDSTSDSNNIIDFIYKNLPQTNINQLLTKNKYDDNYSLTTLIQNLFNLNSDISDYEQPRNGEKSTNDSNKNSDNSIKNDTDTQSSKQDKADNQKAPKSNNTKPSTSNKQPNSPKPTQPNQSNSQPASDDKANQKSSSKDNQSMSDSALDSILDQYSEDAKKTQKDYASQSKKDKNEKSNTKNPQLPTQDELKHKSKPAQSFNNDVNQKDTRATSLFETDPSISNNDDSGQFNVVDSKDTRQFVKSIAKDAHRIGQDNDIYASVMIAQAILESDSGRSALAKSPNHNLFGIKGAFEGNSVPFNTLEADGNQLYSINAGFRKYPSTKESLKDYSDLIKNGIDGNRTIYKPTWKSEADSYKDATSHLSKTYATDPNYAKKLNSIIKHYQLTQFDDERMPDLDKYERSIKDYDDSSDEFKPFREVSDSMPYPHGQCTWYVYNRMKQFGTSISGDLGDAHNWNNRAQYRDYQVSHTPKRHAAVVFEAGQFGADQHYGHVAFVEKVNSDGSIVISESNVKGLGIISHRTINAAAAEELSYITGK

Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

Expression & Regulation[edit | edit source]

Operon[edit | edit source]

Regulation[edit | edit source]

  • regulator:

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]