From AureoWiki
Jump to navigation Jump to search
PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL0897 [new locus tag: SACOL_RS04605 ]
  • pan locus tag?: SAUPAN002859000
  • symbol: SACOL0897
  • pan gene symbol?:
  • synonym:
  • product: hypothetical protein

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SACOL0897 [new locus tag: SACOL_RS04605 ]
  • symbol: SACOL0897
  • product: hypothetical protein
  • replicon: chromosome
  • strand: +
  • coordinates: 909954..911411
  • length: 1458
  • essential: unknown

Accession numbers[edit | edit source]

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    TTGGAAGATGTTACAAACGAAGAAGTATTTGAAATGATTGATAGCAGAACCGGTGTTTTA
    AATGCTAATGATTGGAAAAGTCAATTAAGGCGTTCTGCTACTACACAAGCATTGAAAAAA
    ACGACTACAAATGCTGAAATCATATTGTGTAATGATGAGAGTTTAAAAGGGCTAGTACAA
    TATGACGCTTTTGAAAAAGTAACCAAACTGAAACGTCTACCGTATTGGAGGTCAAAAGGA
    GATACGAATTATTATTGGGCTGATATAGATACCACACATGTGATTTCACATATTGATAAA
    TTGTATAATGTGCAGTTTAGCCGTGATCTTATTGATACTGTAATTGAAAAGGAAGCTTAT
    CAAAATAGATTTCACCCTATTAAATCGATGATTGAATCTAAATCATGGGACGGAATCAAA
    AGAATTGAAACGCTCTTCATTGATTATTTAGGTGCTGAAGATAACCACTATAATAGAGAA
    GTTACAAAAAAATGGATGATGGGCGCAGTTGCTAGAATCTATCAGCCAGGTATTAAATAT
    GATTCCATGATTATTTTATATGGTGGTCAAGGTGTTGGAAAATCTACGGCAGTGAGTAAA
    TTGGGAGGTCATTGGTATAACCAAAGTATTAAAACGTTTAAAGGTGATGAGGTCTATAAG
    AAATTGCAGGGTTCTTGGATATGTGAAATTGAAGAACTGTCGGCATTTCAAAAGTCTACT
    ATTGAAGATATTAAGGGGTTTATAAGTGCTATTGTAGATATTTATAGAGCTTCGTATGGT
    AAACGAACAGAGCGTCATCCTAGACAGTGTGTGTTTGTAGGGACAACCAATAACTATGAG
    TTTTTAAAAGACCAAACAGGCAATCGTCGTTTTTTCCCTATTACGACAGATAAAAATAAA
    GCAACTAAAAGCCCGTTTGACGATCTAACACCAGATGTTGTGCAACAAATGTTTGCTGAA
    GCTAAAGTATATTTTGATGAGGATCCGACGGATAAAGCATTGTTATTAGATAAAGAAGCG
    AGTGAGATGGCTTTAAAAGTCCAAGAAGCTCATTCTGAAAAAGATGCTTTAGTTGGAGAA
    ATAGAAGAATTCCTTGAACGTCCTATTCCGTCAGACTATTGGTATAGAACGTTAGAAGAA
    AAAAGAGTGTCTGCGCATGATGTTATAGACCAAGACTATATTAAATTATATGGTGATGGT
    AAATTGATTGAATTACCGAATACAAAACCAGGTGCTTATGTATGGCGTGACAAGGTATGT
    AGCATGGAAATTTGGAAAGTGATGATGAAACGAGATGACCAACCACAACAACACCATTTA
    AGAAAAATTGATAAAGCGTTAAGAAATACAAATTATTGTGACACTGTGAAAAAGCAAACG
    CGATATGGTGAAGGTATTGGTAAGCAATATGGCTTTAGTGTAGATTTAGCTTCTTATTAT
    AAGAATCTTAAAGTTTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1458

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SACOL0897 [new locus tag: SACOL_RS04605 ]
  • symbol: SACOL0897
  • description: hypothetical protein
  • length: 485
  • theoretical pI: 7.05745
  • theoretical MW: 56364.5
  • GRAVY: -0.661856

Function[edit | edit source]

  • TIGRFAM:
    Cellular processes Cellular processes Chemotaxis and motility flagellar biosynthesis protein FlhF (TIGR03499; HMM-score: 16.8)
  • TheSEED  :
    • Hypothetical SAV0791 homolog in superantigen-encoding pathogenicity islands SaPI
    Phages, Prophages, Transposable elements, Plasmids Pathogenicity islands Staphylococcal pathogenicity islands SaPI  Hypothetical SAV0791 homolog in superantigen-encoding pathogenicity islands SaPI
  • PFAM:
    P-loop_NTPase (CL0023) VirE; Virulence-associated protein E (PF05272; HMM-score: 241.3)
    and 6 more
    RNA_helicase; RNA helicase (PF00910; HMM-score: 17.9)
    AAA_24; AAA domain (PF13479; HMM-score: 16.2)
    AAA; ATPase family associated with various cellular activities (AAA) (PF00004; HMM-score: 14.7)
    AAA_28; AAA domain (PF13521; HMM-score: 14.3)
    AAA_16; AAA ATPase domain (PF13191; HMM-score: 12.3)
    SRP54; SRP54-type protein, GTPase domain (PF00448; HMM-score: 11.9)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.012039
    • TAT(Tat/SPI): 0.00936
    • LIPO(Sec/SPII): 0.000837
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MEDVTNEEVFEMIDSRTGVLNANDWKSQLRRSATTQALKKTTTNAEIILCNDESLKGLVQYDAFEKVTKLKRLPYWRSKGDTNYYWADIDTTHVISHIDKLYNVQFSRDLIDTVIEKEAYQNRFHPIKSMIESKSWDGIKRIETLFIDYLGAEDNHYNREVTKKWMMGAVARIYQPGIKYDSMIILYGGQGVGKSTAVSKLGGHWYNQSIKTFKGDEVYKKLQGSWICEIEELSAFQKSTIEDIKGFISAIVDIYRASYGKRTERHPRQCVFVGTTNNYEFLKDQTGNRRFFPITTDKNKATKSPFDDLTPDVVQQMFAEAKVYFDEDPTDKALLLDKEASEMALKVQEAHSEKDALVGEIEEFLERPIPSDYWYRTLEEKRVSAHDVIDQDYIKLYGDGKLIELPNTKPGAYVWRDKVCSMEIWKVMMKRDDQPQQHHLRKIDKALRNTNYCDTVKKQTRYGEGIGKQYGFSVDLASYYKNLKV

Experimental data[edit | edit source]

  • experimentally validated:
  • protein localization: Cytoplasmic [1]
  • quantitative data / protein copy number per cell:
  • interaction partners:

Expression & Regulation[edit | edit source]

Regulation[edit | edit source]

  • regulator:

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

  1. Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int J Med Microbiol: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)

Relevant publications[edit | edit source]