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NCBI: 03-AUG-2016

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_00326
  • pan locus tag?: SAUPAN001883000
  • symbol: SAOUHSC_00326
  • pan gene symbol?: fepB
  • synonym:
  • product: hypothetical protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAOUHSC_00326
  • symbol: SAOUHSC_00326
  • product: hypothetical protein
  • replicon: chromosome
  • strand: +
  • coordinates: 338731..339960
  • length: 1230
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    ATGACAAATTATGAACAAGTTAACGATAGTACGCAATTTTCAAGACGTACATTTTTGAAA
    ATGTTAGGTATTGGCGGTGCCGGTGTTGCAATTGGCGCAAGTGGTGTTGGTAGCATGTGG
    TCTTTCAAATCAATGTTCAATACACCAGAAGATCCGGAAAAAGATGCGTATGAATTTTAT
    GGTAAAGTGCAACCAGGCATTACCACACCCACGCAAAAAACATGCAATTTCGTTGCGTTA
    GATTTGAAGTCAAAAGATAGAGATGCAATTAAGGCAATGTTTAAAAAGTGGACGGTTATG
    GCTGATCGTATGATGGATGGTGATACAGTTGGCAAGCCGAGTAACAATCCTTTAATGCCA
    CCAGTAGATACCGGTGAATCGATAGGATTAGGTGCAAGCAAGTTAACGATTACCTTTGGG
    ATTAGTAAGTCTTTGATGAAGAAAATTGGGTTATCTAGTAAAATTCCCGATGCCTTTAAA
    GATTTACCGCATTTTCCGAATGATCAGTTAATAGACGATTACAGCGATGGTGATATTATG
    ATTCAAGCATGCTCAAATGATTCGCAAGTATCCTTTCATGCGGTTCATAATTTAGTTCGT
    CCATTTCGAGATATTGTTAAGGTACGTTGGGCGCAATCTGGTTTTATCTCTGCTAAAGGT
    AAGGAAACACCTAGAAATTTAATGGCATTTAAAGATGGAACAATTAATCCTAGGAAGAAT
    AATCAACTTAAAGATTATGTGTTTATTGATGACGGATGGGCGAAACATGGAACTTATTGC
    GTTGTCAGACGTATTCAAATACACATTGAAACGTGGGATCGTACTGCGCTGGAAGAACAA
    GAGGCTACATTTGGTCGGAAACGACATAGTGGTGCACCGTTAACAGGTGGGAAAGAGTTT
    GATGAAATTGACTTAAAAGCGAAAGATAGTCATGGCGAGTATATTATTGATAAAGATGCC
    CATACGAGGCTAGCGAAAGAAGCAAATACGTCAATTTTACGTAGAGCCTTTAATTATGTG
    GATGGTACGGATGACCGCACAGGTAACTTCGAAACAGGCTTACTTTTTATTGCTTTTCAA
    AAAGCGACAAAACAATTTATCGATATACAAAATAATTTAGGTAGTAATGATAAATTAAAT
    GAATATATTACACATAGAGGTTCTGCTTCATTTTTAGTATTACCAGGTGTTAGTAAGGGA
    GGATACCTTGGTGAAACATTATTTGACTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1230

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAOUHSC_00326
  • symbol: SAOUHSC_00326
  • description: hypothetical protein
  • length: 409
  • theoretical pI: 8.08612
  • theoretical MW: 45558.3
  • GRAVY: -0.480685

⊟Function[edit | edit source]

  • TIGRFAM:
    Tat-translocated enzyme (TIGR01412; HMM-score: 598.6)
    and 3 more
    Dyp-type peroxidase family (TIGR01413; HMM-score: 330.6)
    Genetic information processing Protein fate Protein and peptide secretion and trafficking Tat (twin-arginine translocation) pathway signal sequence (TIGR01409; HMM-score: 25.5)
    Unknown function General formate dehydrogenase region TAT target (TIGR02811; HMM-score: 13.9)
  • TheSEED  :
    • Ferrous iron transport peroxidase EfeB
    Iron acquisition and metabolism Iron acquisition and metabolism - no subcategory Ferrous iron transporter EfeUOB, low-pH-induced  Ferrous iron transport peroxidase EfeB
  • PFAM:
    Dim_A_B_barrel (CL0032) Dyp_perox; Dyp-type peroxidase family (PF04261; HMM-score: 390.6)
    and 1 more
    TAT (CL0300) TAT_signal; TAT (twin-arginine translocation) pathway signal sequence (PF10518; HMM-score: 20.9)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Extracellular
    • Cytoplasmic Score: 0.1
    • Cytoplasmic Membrane Score: 0.14
    • Cellwall Score: 0.15
    • Extracellular Score: 9.6
    • Internal Helix: 1
  • LocateP: N-terminally anchored (No CS)
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Possibly Tat-/Sec-(SPI)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: -0.5
    • N-terminally Anchored Score: 5
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: Signal peptide TAT(Tat/SPI) length 41 aa
    • SP(Sec/SPI): 0.002268
    • TAT(Tat/SPI): 0.988184
    • LIPO(Sec/SPII): 0.003962
    • Cleavage Site: CS pos: 41-42. MWS-FK. Pr: 0.3841
  • predicted transmembrane helices (TMHMM): 1

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MTNYEQVNDSTQFSRRTFLKMLGIGGAGVAIGASGVGSMWSFKSMFNTPEDPEKDAYEFYGKVQPGITTPTQKTCNFVALDLKSKDRDAIKAMFKKWTVMADRMMDGDTVGKPSNNPLMPPVDTGESIGLGASKLTITFGISKSLMKKIGLSSKIPDAFKDLPHFPNDQLIDDYSDGDIMIQACSNDSQVSFHAVHNLVRPFRDIVKVRWAQSGFISAKGKETPRNLMAFKDGTINPRKNNQLKDYVFIDDGWAKHGTYCVVRRIQIHIETWDRTALEEQEATFGRKRHSGAPLTGGKEFDEIDLKAKDSHGEYIIDKDAHTRLAKEANTSILRRAFNYVDGTDDRTGNFETGLLFIAFQKATKQFIDIQNNLGSNDKLNEYITHRGSASFLVLPGVSKGGYLGETLFD

⊟Experimental data[edit | edit source]

  • experimentally validated:
  • protein localization:
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: Fur* (repression) regulon
    Fur*(TF)important in Iron homeostasis; RegPrecise    transcription unit transferred from N315 data RegPrecise 

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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