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NCBI: 03-AUG-2016
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus NCTC8325
- locus tag: SAOUHSC_02305
- pan locus tag?: SAUPAN005341000
- symbol: SAOUHSC_02305
- pan gene symbol?: alr
- synonym:
- product: alanine racemase
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
⊟Accession numbers[edit | edit source]
- Gene ID: 3920930 NCBI
- RefSeq: YP_500784 NCBI
- BioCyc: G1I0R-2177 BioCyc
- MicrobesOnline: 1290745 MicrobesOnline
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
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1141ATGTCGGATAAATATTATAGATCTGCGTATATGAATGTAGATTTAAACGCTGTTGCATCA
AATTTCAAAGTATTCAGTACATTGCATCCAAATAAAACAGTGATGGCTGTCGTTAAAGCC
AATGCCTATGGACTAGGTAGTGTTAAAGTAGCACGTCATTTAATGGAAAATGGCGCCACA
TTTTTTGCTGTAGCAACGTTAGATGAAGCGATAGAACTTAGAATGCATGGGATTACTGCT
AAAATTTTAGTCTTAGGTGTGTTACCAGCTAAAGATATTGATAAAGCGATACAACACCGA
GTTGCCTTAACGGTTCCGTCTAAACAGTGGTTGAAAGAAGCAATTAAAAACATTTCTGGT
GAGCAAGAGAAAAAGTTATGGTTGCACATTAAATTAGATACAGGAATGGGACGTTTAGGT
ATTAAAGATACTAAAACGTATCAAGAAGTGATTGAAATCATTCAACAATATGAGCAACTT
GTATTTGAAGGCGTGTTTACACACTTTGCCTGTGCTGACGAACCAGGAGATATGACAACT
GAACAATATCAACGTTTTAAAGATATGGTCAATGAAGCAATTAAACCTGAATATATACAT
TGTCAGAACTCAGCAGGCTCTCTATTAATGGATTGCCAATTCTGTAATGCAATAAGACCA
GGAATTTCCCTTTATGGATATTATCCATCAGAGTATGTACAGCAAAAAGTTAAAGTACAC
CTTAAACCAAGTGTGCAATTAATTGCTAATGTAGTTCAAACAAAGACGCTACAAGCGGGT
GAGTCTGTAAGTTATGGTGCAACTTATACAGCTACTGACCCAACTACAATAGCATTGTTA
CCTATTGGATATGCAGATGGCTATTTACGCATAATGCAAGGTAGCTTTGTAAATGTAAAT
GGTCATCAATGCGAAGTTATTGGTCGCGTATGTATGGATCAGACAATTGTTAAAGTGCCA
GATCAAGTTAAAGCTGGAGATTCGGTGATTTTAATAGATAATCATAGAGAAAGTCCACAG
TCGGTAGAGGTGGTAGCTGAAAAGCAACATACTATTAATTATGAAGTGCTTTGTAACTTG
TCGAGACGTTTGCCGCGAATCTATCATGATGGTGATCAACGTTTTGTAACAAATGAATTG
TTAAAATAA60
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⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: SAOUHSC_02305
- symbol: SAOUHSC_02305
- description: alanine racemase
- length: 382
- theoretical pI: 7.33759
- theoretical MW: 42823
- GRAVY: -0.158377
⊟Function[edit | edit source]
- reaction: EC 5.1.1.1? ExPASyAlanine racemase L-alanine = D-alanine
- TIGRFAM: Cell envelope Biosynthesis and degradation of murein sacculus and peptidoglycan alanine racemase (TIGR00492; EC 5.1.1.1; HMM-score: 373.1)and 1 morePRTRC system protein B (TIGR03737; HMM-score: 12.2)
- TheSEED :
- Alanine racemase (EC 5.1.1.1)
Amino Acids and Derivatives Alanine, serine, and glycine Alanine biosynthesis Alanine racemase (EC 5.1.1.1)and 1 more - PFAM: TIM_barrel (CL0036) Ala_racemase_N; Alanine racemase, N-terminal domain (PF01168; HMM-score: 223.5)and 2 moreno clan defined Ala_racemase_C; Alanine racemase, C-terminal domain (PF00842; HMM-score: 143.5)Styelin; Styelin A-E (PF17562; HMM-score: 12.7)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors: pyridoxal 5'-phosphate
- effectors:
⊟Localization[edit | edit source]
- PSORTb: Cytoplasmic
- Cytoplasmic Score: 9.89
- Cytoplasmic Membrane Score: 0.09
- Cellwall Score: 0.01
- Extracellular Score: 0.02
- Internal Helices: 0
- LocateP: Intracellular
- Prediction by SwissProt Classification: Cytoplasmic
- Pathway Prediction: No pathway
- Intracellular possibility: 1
- Signal peptide possibility: -1
- N-terminally Anchored Score: 1
- Predicted Cleavage Site: No CleavageSite
- SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.014203
- TAT(Tat/SPI): 0.002283
- LIPO(Sec/SPII): 0.000643
- predicted transmembrane helices (TMHMM): 0
⊟Accession numbers[edit | edit source]
⊟Protein sequence[edit | edit source]
- MSDKYYRSAYMNVDLNAVASNFKVFSTLHPNKTVMAVVKANAYGLGSVKVARHLMENGATFFAVATLDEAIELRMHGITAKILVLGVLPAKDIDKAIQHRVALTVPSKQWLKEAIKNISGEQEKKLWLHIKLDTGMGRLGIKDTKTYQEVIEIIQQYEQLVFEGVFTHFACADEPGDMTTEQYQRFKDMVNEAIKPEYIHCQNSAGSLLMDCQFCNAIRPGISLYGYYPSEYVQQKVKVHLKPSVQLIANVVQTKTLQAGESVSYGATYTATDPTTIALLPIGYADGYLRIMQGSFVNVNGHQCEVIGRVCMDQTIVKVPDQVKAGDSVILIDNHRESPQSVEVVAEKQHTINYEVLCNLSRRLPRIYHDGDQRFVTNELLK
⊟Experimental data[edit | edit source]
- experimentally validated: PeptideAtlas [2] [3]
- protein localization: data available for COL
- quantitative data / protein copy number per cell: data available for COL
- interaction partners:
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
- MicrobesOnline: SAOUHSC_02305 < acpS < SAOUHSC_02307 < SAOUHSC_02308 < SAOUHSC_02309predicted SigA promoter [4] : SAOUHSC_02298 < SAOUHSC_02299 < SAOUHSC_02300 < S890 < SAOUHSC_02301 < S891 < SAOUHSC_02303 < SAOUHSC_02304 < SAOUHSC_02305 < acpS < SAOUHSC_02307 < SAOUHSC_02308 < SAOUHSC_02309 < S892
⊟Regulation[edit | edit source]
- regulator:
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: [4]
Multi-gene expression profiles
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
- Aureolib: no data available
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
You are kindly invited to share additional interesting facts.
⊟Literature[edit | edit source]
⊟References[edit | edit source]
- ↑ Roy R Chaudhuri, Andrew G Allen, Paul J Owen, Gil Shalom, Karl Stone, Marcus Harrison, Timothy A Burgis, Michael Lockyer, Jorge Garcia-Lara, Simon J Foster, Stephen J Pleasance, Sarah E Peters, Duncan J Maskell, Ian G Charles
Comprehensive identification of essential Staphylococcus aureus genes using Transposon-Mediated Differential Hybridisation (TMDH).
BMC Genomics: 2009, 10;291
[PubMed:19570206] [WorldCat.org] [DOI] (I e) - ↑ Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
Proteomics: 2015, 15(21);3648-61
[PubMed:26224020] [WorldCat.org] [DOI] (I p) - ↑ Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
Sci Rep: 2017, 7(1);9718
[PubMed:28887440] [WorldCat.org] [DOI] (I e) - ↑ 4.0 4.1 Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
PLoS Genet: 2016, 12(4);e1005962
[PubMed:27035918] [WorldCat.org] [DOI] (I e)
⊟Relevant publications[edit | edit source]
I Kullik, R Jenni, B Berger-Bächi
Sequence of the putative alanine racemase operon in Staphylococcus aureus: insertional interruption of this operon reduces D-alanine substitution of lipoteichoic acid and autolysis.
Gene: 1998, 219(1-2);9-17
[PubMed:9756984] [WorldCat.org] [DOI] (P p)