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NCBI: 03-AUG-2016

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_02851
  • pan locus tag?: SAUPAN006198000
  • symbol: SAOUHSC_02851
  • pan gene symbol?: cidA
  • synonym:
  • product: hypothetical protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAOUHSC_02851
  • symbol: SAOUHSC_02851
  • product: hypothetical protein
  • replicon: chromosome
  • strand: -
  • coordinates: 2625749..2626144
  • length: 396
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    ATGCACAAAGTCCAATTAATAATCAAACTACTACTACAACTAGGAATCATCATTGTGATT
    ACTTATATTGGCACAGAAATTCAAAAGATTTTTCATCTTCCCTTAGCCGGCAGTATTGTT
    GGTCTATTTTTATTTTATTTACTATTACAATTTAAGATTGTACCGCTAACTTGGGTAGAA
    GACGGTGCAAACTTTTTATTAAAGACGATGGTCTTTTTCTTCATACCGTCAGTTGTAGGT
    ATTATGGATGTTGCTTCCGAAATTACGCTAAATTATATACTCTTTTTCGCAGTCATTATC
    ATAGGAACATGTATCGTTGCATTATCTTCAGGTTATATTGCTGAAAAAATGTCTGTTAAA
    CATAAACATCGTAAAGGTGTAGACGCTTATGAATGA
    60
    120
    180
    240
    300
    360
    396

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAOUHSC_02851
  • symbol: SAOUHSC_02851
  • description: hypothetical protein
  • length: 131
  • theoretical pI: 9.01426
  • theoretical MW: 14729.8
  • GRAVY: 1.04962

⊟Function[edit | edit source]

  • TIGRFAM:
  • TheSEED  :
    • Holin-like protein CidA
    Regulation and Cell signaling Programmed Cell Death and Toxin-antitoxin Systems Murein hydrolase regulation and cell death  Holin-like protein CidA
  • PFAM:
    no clan defined LrgA; LrgA family (PF03788; HMM-score: 83.4)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 4
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.004866
    • TAT(Tat/SPI): 0.000114
    • LIPO(Sec/SPII): 0.016401
  • predicted transmembrane helices (TMHMM): 4

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MHKVQLIIKLLLQLGIIIVITYIGTEIQKIFHLPLAGSIVGLFLFYLLLQFKIVPLTWVEDGANFLLKTMVFFFIPSVVGIMDVASEITLNYILFFAVIIIGTCIVALSSGYIAEKMSVKHKHRKGVDAYE

⊟Experimental data[edit | edit source]

  • experimentally validated:
  • protein localization:
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ 1.0 1.1 Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

Kelly C Rice, Brian A Firek, Jeremy B Nelson, Soo-Jin Yang, Toni G Patton, Kenneth W Bayles
The Staphylococcus aureus cidAB operon: evaluation of its role in regulation of murein hydrolase activity and penicillin tolerance.
J Bacteriol: 2003, 185(8);2635-43
[PubMed:12670989] [WorldCat.org] [DOI] (P p)Kelly C Rice, Kenneth W Bayles
Death's toolbox: examining the molecular components of bacterial programmed cell death.
Mol Microbiol: 2003, 50(3);729-38
[PubMed:14617136] [WorldCat.org] [DOI] (P p)

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