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PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_2115 [new locus tag: SAUSA300_RS11650 ]
  • pan locus tag?: SAUPAN005511000
  • symbol: tnp
  • pan gene symbol?: tnp4
  • synonym:
  • product: IS1181, transposase

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_2115 [new locus tag: SAUSA300_RS11650 ]
  • symbol: tnp
  • product: IS1181, transposase
  • replicon: chromosome
  • strand: +
  • coordinates: 2290382..2291620
  • length: 1239
  • essential: unknown other strains

Accession numbers[edit | edit source]

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    GTGACCAAACCCAAACTAAAGGAGACAAGTGCGCCTATGTGTAATGATACCTTAGAATTA
    CTAAGAATAAAAGATGAAAATATAAAATATATAAACCAAGAAATTGACGTCATTATCAAA
    GGAAAAAAAGCAACAGTGGTTAATGCTGTACTAACGTATAAGCCTTCGGCCTGTTATTGT
    TGTGGAGTTAAAAATGAAGGACAAATTCATAAACATGGTAAGCGTGTTTCTCGTATTACT
    TTACTTAAAACTCAAGGGTATAACACATACCTCAACTTAGCTAAACAACGTTTTAAATGC
    TTAGAATGCAATGGCACTTTTACTGCTAAAACGTCAATTGTTGATGAGTCGTGTTTTATC
    TCAAGATGTGTTACTCAAAAAGTTATAGAAGAAGCTACTAAAGTTAAAACAGAGATTGAT
    ACTGCAGAAGATAACTGTATCTCTCCATCTACTGTAAGTCGTATTAGAACTAAAGCGGCT
    AATTCATTACGAATTAAACCCTTTAATTGTTTGCCAGAACACATCGCTATGGATGAATTT
    AAAAGCGTTAAAAATGTAACTGGATCAATGAGTTTCATTTTTATAGATAATGATACTCAT
    GATGTTATAGATATTTTAGAAAATAGAACTACAAGATTCTTGCGTGCCTATTTCGAGCGA
    TTCGATTTAAAAAATCGACAACAAGTTAAGACGGTTACTATTGACATGTATGAACCCTAT
    GTCCGATTATTTCGCGACCTATTTCCTAATGCAGCTATTATTTTTGACAGATTCCATATC
    GTTCAACATTTAAATAGAGAACTTAATAAGTATCGTGTACAAGTTATGAATGAATACCGT
    AATAAAAAAGGACCTGATTATACAATTTTTAAGAATAACTGGAAAGTCCTATTGATGGAT
    ACTAGTAAAACCATATTTAGTAAATACAGATGGAATAAATCTTTTAAGGCTTATAAACGC
    TCATCTGACATTGTAGAATTCATGCTTTCAAAAGACGATATACTACGACACTCCTACGAA
    CTTGTCCAAGGATTACGAAAAGACCTAAGGTTATGTAATTGGCCTAAATTTATTAATCGT
    TTAAATTCAGTTAGTAAAAAGTCTGTGAGTAAGGGTGTATGGAAAGTGGTTAAATATTAT
    AGAAAACATCAAAGGATGTTAAGAAATACAATTTATTACCCAGCATTTAATAATGGTGCT
    ATAGAAGGAATTAATAATAAGATAAAATTAATCAAGTGA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1239

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SAUSA300_2115 [new locus tag: SAUSA300_RS11650 ]
  • symbol: Tnp
  • description: IS1181, transposase
  • length: 412
  • theoretical pI: 10.2563
  • theoretical MW: 48326.9
  • GRAVY: -0.486893

Function[edit | edit source]

  • TIGRFAM:
    Cellular processes Cellular processes Detoxification S-(hydroxymethyl)glutathione synthase (TIGR02820; EC 4.4.1.22; HMM-score: 16.8)
    Metabolism Central intermediary metabolism One-carbon metabolism S-(hydroxymethyl)glutathione synthase (TIGR02820; EC 4.4.1.22; HMM-score: 16.8)
  • TheSEED  :
    • Mobile element protein
  • PFAM:
    RNase_H (CL0219) DDE_Tnp_ISL3; Transposase (PF01610; HMM-score: 144.9)
    and 4 more
    Zn_Beta_Ribbon (CL0167) zf-ISL3; zinc-finger of transposase IS204/IS1001/IS1096/IS1165 (PF14690; HMM-score: 42.5)
    HTH (CL0123) HTH_Tnp_ISL3; Helix-turn-helix domain of transposase family ISL3 (PF13542; HMM-score: 20.7)
    no clan defined DUF3795; Protein of unknown function (DUF3795) (PF12675; HMM-score: 10.4)
    DUF4163; Domain of unknown function (DUF4163) (PF13739; HMM-score: 10.3)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.048614
    • TAT(Tat/SPI): 0.001594
    • LIPO(Sec/SPII): 0.005897
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MTKPKLKETSAPMCNDTLELLRIKDENIKYINQEIDVIIKGKKATVVNAVLTYKPSACYCCGVKNEGQIHKHGKRVSRITLLKTQGYNTYLNLAKQRFKCLECNGTFTAKTSIVDESCFISRCVTQKVIEEATKVKTEIDTAEDNCISPSTVSRIRTKAANSLRIKPFNCLPEHIAMDEFKSVKNVTGSMSFIFIDNDTHDVIDILENRTTRFLRAYFERFDLKNRQQVKTVTIDMYEPYVRLFRDLFPNAAIIFDRFHIVQHLNRELNKYRVQVMNEYRNKKGPDYTIFKNNWKVLLMDTSKTIFSKYRWNKSFKAYKRSSDIVEFMLSKDDILRHSYELVQGLRKDLRLCNWPKFINRLNSVSKKSVSKGVWKVVKYYRKHQRMLRNTIYYPAFNNGAIEGINNKIKLIK

Experimental data[edit | edit source]

  • experimentally validated:
  • protein localization:
  • quantitative data / protein copy number per cell:
  • interaction partners:

Expression & Regulation[edit | edit source]

Operon[edit | edit source]

Regulation[edit | edit source]

  • regulator:

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]