NCBI: 10-JUN-2013
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus USA300_FPR3757
- locus tag: SAUSA300_0491 [new locus tag: SAUSA300_RS02635 ]
- pan locus tag?: SAUPAN002255000
- symbol: cysK
- pan gene symbol?: cysK
- synonym:
- product: cysteine synthase A
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: SAUSA300_0491 [new locus tag: SAUSA300_RS02635 ]
- symbol: cysK
- product: cysteine synthase A
- replicon: chromosome
- strand: +
- coordinates: 550846..551778
- length: 933
- essential: unknown other strains
⊟Accession numbers[edit | edit source]
- Gene ID: 3912911 NCBI
- RefSeq: YP_493200 NCBI
- BioCyc: see SAUSA300_RS02635
- MicrobesOnline: 1292006 MicrobesOnline
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
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901ATGGCACAAAAACCAGTAGATAATATTACTCAAATTATTGGCGGTACACCGGTAGTCAAA
TTGAGAAATGTAGTAGATGACAATGCAGCAGATGTTTATGTAAAATTGGAATATCAAAAT
CCAGGTGGTTCTGTAAAGGATAGAATTGCTTTAGCAATGATTGAAAAAGCAGAGCGAGAA
GGCAAAATTAAACCTGGCGATACAATTGTAGAACCAACAAGTGGTAATACAGGTATCGGT
TTAGCATTTGTATGTGCTGCTAAAGGATATAAAGCAGTATTTACTATGCCCGAAACAATG
AGCCAAGAGCGTCGTAATTTATTAAAAGCATACGGTGCGGAATTAGTTTTAACGCCTGGA
TCAGAAGCGATGAAAGGTGCAATTAAAAAAGCTAAAGAATTGAAAGAAGAACATGGTTAC
TTCGAGCCACAACAATTTGAAAACCCTGCGAACCCTGAAGTTCATGAGTTAACTACAGGT
CCTGAGTTATTACAACAATTTGAAGGGAAAACTATCGATGCGTTCCTAGCTGGTGTTGGT
ACTGGTGGTACGTTATCTGGTGTAGGTAAAGTTCTGAAAAAAGAATATCCTAACATCGAA
ATTGTTGCTATAGAGCCTGAGGCTTCTCCAGTATTGAGCGGTGGTGAGCCAGGTCCACAT
AAATTACAAGGTTTAGGTGCTGGATTTATTCCAGGCACTTTGAATACAGAAATCTATGAC
AGTATTATTAAAGTAGGAAATGATACAGCGATGGAAATGTCTCGTCGAGTTGCTAAAGAG
GAAGGTATTTTAGCAGGTATTTCATCAGGTGCTGCGATTTATGCTGCCATTCAAAAAGCA
AAAGAATTAGGAAAAGGTAAAACAGTAGTAACAGTATTGCCGAGTAATGGTGAACGCTAC
TTATCAACACCTTTATATTCATTCGATGACTAA60
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⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: SAUSA300_0491 [new locus tag: SAUSA300_RS02635 ]
- symbol: CysK
- description: cysteine synthase A
- length: 310
- theoretical pI: 5.12885
- theoretical MW: 32975.4
- GRAVY: -0.237419
⊟Function[edit | edit source]
- ⊞reaction: EC 2.5.1.47? ExPASy
- ⊞TIGRFAM: Amino acid biosynthesis Serine family cysteine synthase A (TIGR01139; EC 2.5.1.47; HMM-score: 463.6)Amino acid biosynthesis Serine family cysteine synthase (TIGR01136; EC 2.5.1.47; HMM-score: 440.6)and 12 more
- ⊞TheSEED :
- Cysteine synthase (EC 2.5.1.47)
Amino Acids and Derivatives Lysine, threonine, methionine, and cysteine Cysteine Biosynthesis Cysteine synthase (EC 2.5.1.47)and 1 more - ⊞PFAM: no clan defined PALP; Pyridoxal-phosphate dependent enzyme (PF00291; HMM-score: 241.3)and 3 more
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors: pyridoxal 5'-phosphate
- effectors:
⊟Localization[edit | edit source]
⊟Accession numbers[edit | edit source]
⊟Protein sequence[edit | edit source]
- MAQKPVDNITQIIGGTPVVKLRNVVDDNAADVYVKLEYQNPGGSVKDRIALAMIEKAEREGKIKPGDTIVEPTSGNTGIGLAFVCAAKGYKAVFTMPETMSQERRNLLKAYGAELVLTPGSEAMKGAIKKAKELKEEHGYFEPQQFENPANPEVHELTTGPELLQQFEGKTIDAFLAGVGTGGTLSGVGKVLKKEYPNIEIVAIEPEASPVLSGGEPGPHKLQGLGAGFIPGTLNTEIYDSIIKVGNDTAMEMSRRVAKEEGILAGISSGAAIYAAIQKAKELGKGKTVVTVLPSNGERYLSTPLYSFDD
⊟Experimental data[edit | edit source]
- experimentally validated: data available for COL, NCTC8325
- protein localization: data available for COL
- quantitative data / protein copy number per cell: data available for COL
- ⊟interaction partners:
SAUSA300_2142 (asp23) alkaline shock protein 23 [1] (data from MRSA252) SAUSA300_0760 (eno) phosphopyruvate hydratase [1] (data from MRSA252) SAUSA300_0532 (fusA) elongation factor G [1] (data from MRSA252) SAUSA300_0756 (gap) glyceraldehyde-3-phosphate dehydrogenase, type I [1] (data from MRSA252) SAUSA300_0996 (lpdA) dihydrolipoamide dehydrogenase [1] (data from MRSA252) SAUSA300_2541 (mqo) malate:quinone oxidoreductase [1] (data from MRSA252) SAUSA300_0993 (pdhA) pyruvate dehydrogenase E1 component, alpha subunit [1] (data from MRSA252) SAUSA300_0994 (pdhB) pyruvate dehydrogenase E1 component, beta subunit [1] (data from MRSA252) SAUSA300_2089 (pdp) pyrimidine-nucleoside phosphorylase [1] (data from MRSA252) SAUSA300_1644 (pyk) pyruvate kinase [1] (data from MRSA252) SAUSA300_0523 (rplA) 50S ribosomal protein L1 [1] (data from MRSA252) SAUSA300_0524 (rplJ) 50S ribosomal protein L10 [1] (data from MRSA252) SAUSA300_0533 (tuf) elongation factor Tu [1] (data from MRSA252) SAUSA300_1583 hypothetical protein [1] (data from MRSA252)
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
- MicrobesOnline: no polycistronic organisation predicted
⊟Regulation[edit | edit source]
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: data available for NCTC8325
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
⊟Protein stability[edit | edit source]
- half-life: no data available
⊞Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊞Other Information[edit | edit source]
⊟Literature[edit | edit source]
⊟References[edit | edit source]
- ↑ Jump up to: 1.00 1.01 1.02 1.03 1.04 1.05 1.06 1.07 1.08 1.09 1.10 1.11 1.12 1.13 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
J Proteome Res: 2011, 10(3);1139-50
[PubMed:21166474] [WorldCat.org] [DOI] (I p)