PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_1583 [new locus tag: SAUSA300_RS08625 ]
• pan locus tag^?: SAUPAN004228000
• symbol: SAUSA300_1583
• pan gene symbol^?: cymR
• synonym:
• product: hypothetical protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_1583 [new locus tag: SAUSA300_RS08625 ]
• symbol: SAUSA300_1583
• product: hypothetical protein
• replicon: chromosome
• strand: -
• coordinates: 1732929..1733351
• length: 423
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 3913642 NCBI
• RefSeq: YP_494278 NCBI
• BioCyc: see SAUSA300_RS08625
• MicrobesOnline: 1293098 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  361
  421
  
  ATGAAAATTTCTACTAAAGGGAGATATGGACTTACATTGATGATTTCTCTTGCTAAAAAA
  GAGGGGCAAGGATGTATATCATTAAAGTCAATTGCTGAAGAAAATAATTTGAGTGATTTA
  TATTTAGAACAGCTTGTAGGTCCTTTAAGAAATGCGGGGTTAATTCGAAGTGTACGCGGT
  GCTAAAGGTGGATACCAATTAAGAGTGCCAGCGGAAGAAATCTCAGCAGGGGATATTATA
  AGACTGTTAGAAGGTCCAATTACATTTGTTGAAAGTATTGAATCAGAACCACCTGCGCAA
  AAACAACTATGGATTCGCATGAGAGATGCAGTGAGAGATGTTTTAGATAATACAACATTG
  AAATATTTAGCGGAATATGTAGATACAAGTGAAGATTTAGACGGATACATGTTTTATATT
  TAA
  60
  120
  180
  240
  300
  360
  420
  423
  

⊟Protein

⊟General

• locus tag: SAUSA300_1583 [new locus tag: SAUSA300_RS08625 ]
• symbol: SAUSA300_1583
• description: hypothetical protein
• length: 140
• theoretical pI: 4.79134
• theoretical MW: 15642.9
• GRAVY: -0.175

⊟Function

• TIGRFAM:
  Unknown function General Rrf2 family protein (TIGR00738; HMM-score: 108.5)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Other iron-sulfur cluster assembly transcription factor IscR (TIGR02010; HMM-score: 88.5)
  Signal transduction Regulatory functions DNA interactions iron-sulfur cluster assembly transcription factor IscR (TIGR02010; HMM-score: 88.5)
  and 2 more
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Other FeS assembly SUF system regulator (TIGR02944; HMM-score: 50.2)
  Signal transduction Regulatory functions DNA interactions FeS assembly SUF system regulator (TIGR02944; HMM-score: 50.2)
• TheSEED  :
  • Iron-sulfur cluster regulator IscR
  RNA Metabolism Transcription Rrf2 family transcriptional regulators  Iron-sulfur cluster regulator IscR
  and 2 more
  Stress Response Stress Response - no subcategory Flavohaemoglobin  Iron-sulfur cluster regulator IscR
  Sulfur Metabolism Organic sulfur assimilation At5g37530 (CsdL protein family)  Iron-sulfur cluster regulator IscR
• PFAM:
  HTH (CL0123) Rrf2; Transcriptional regulator (PF02082; HMM-score: 94)
  and 3 more
  HrcA_DNA-bdg; Winged helix-turn-helix transcription repressor, HrcA DNA-binding (PF03444; HMM-score: 17.8)
  MarR_2; MarR family (PF12802; HMM-score: 12.8)
  no clan defined PPO1_DWL; Polyphenol oxidase middle domain (PF12142; HMM-score: 12.1)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors: binding of CysK (cysteine synthetase); O-acetyl-L-serine
• genes regulated by CymR*, TF important in Cysteine metabolismRegPrecise

  repression

  SAUSA300_0173

  gisA*

  tcyP*

  cysM > metB

  metN2* > metP2* > metQ2*

  cysK

  SAUSA300_1998

  luxS

  ydbM*

  tcyC* < tcyB* < tcyA*

  cysG* < cysJ*

  transcription units transferred from N315 data RegPrecise

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.065604
  • TAT(Tat/SPI): 0.001286
  • LIPO(Sec/SPII): 0.06428
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 87161573 NCBI
• RefSeq: YP_494278 NCBI
• UniProt: A0A0H2XJ48 UniProt

⊟Protein sequence

• MKISTKGRYGLTLMISLAKKEGQGCISLKSIAEENNLSDLYLEQLVGPLRNAGLIRSVRGAKGGYQLRVPAEEISAGDIIRLLEGPITFVESIESEPPAQKQLWIRMRDAVRDVLDNTTLKYLAEYVDTSEDLDGYMFYI

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: no polycistronic organisation predicted

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: data available for COL

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

CymR is a cysteine metabolism transcriptional repressor and an oxidative stress sensor. It's proposed consensus operator from RegPrecise is: TWAWWCYKA-nnnnnnnnn-TMRGAWTWA

⊟Literature

⊟References

⊟Relevant publications

Olga Soutourina, Olivier Poupel, Jean-Yves Coppée, Antoine Danchin, Tarek Msadek, Isabelle Martin-Verstraete
CymR, the master regulator of cysteine metabolism in Staphylococcus aureus, controls host sulphur source utilization and plays a role in biofilm formation.
Mol Microbiol: 2009, 73(2);194-211
[PubMed:19508281] [WorldCat.org] [DOI] (I p)Olga Soutourina, Sarah Dubrac, Olivier Poupel, Tarek Msadek, Isabelle Martin-Verstraete
The pleiotropic CymR regulator of Staphylococcus aureus plays an important role in virulence and stress response.
PLoS Pathog: 2010, 6(5);e1000894
[PubMed:20485570] [WorldCat.org] [DOI] (I e)