PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_0437 [new locus tag: SAUSA300_RS02340 ]
• pan locus tag^?: SAUPAN002178000
• symbol: SAUSA300_0437
• pan gene symbol^?: metQ2
• synonym:
• product: NLPA lipoprotein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_0437 [new locus tag: SAUSA300_RS02340 ]
• symbol: SAUSA300_0437
• product: NLPA lipoprotein
• replicon: chromosome
• strand: +
• coordinates: 490505..491347
• length: 843
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 3914608 NCBI
• RefSeq: YP_493150 NCBI
• BioCyc: see SAUSA300_RS02340
• MicrobesOnline: 1291952 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
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  ATGAAAAGATTGATTGGGTTAGTTATCGTAGCACTTGTATTATTAGCAGCGTGTGGTGGT
  AACAATGATAAAAAAGTAACAATTGGTGTCGCATCAAATGACACTAAGGCTTGGGAGAAG
  GTTAAAGAATTAGCTAAAAAAGATGATATTGATGTGGAGATTAAGCACTTCTCAGATTAC
  AATTTACCGAATAAAGCATTAAATGATGGTGATATTGATATGAATGCATTCCAACATTTT
  GCATTTTTAGATCAATATAAAAAGGCGCATAAAGGAACAAAGATTTCAGCATTAAGTACA
  ACAGTTTTAGCACCGTTGGGCATTTACTCAGATAAAATTAAAGATGTCAAAAAGGTTAAA
  GATGGTGCTAAAGTTGTCATTCCAAATGATGTGTCAAACCAAGCACGTGCACTTAAACTA
  TTAGAAGCAGCTGGTTTAATAAAACTGAAAAAAGATTTCGGATTAGCAGGCACGGTGAAA
  GATATAACGTCAAATCCAAAACATTTAAAAATTACTGCAGTAGATGCACAACAAACTGCA
  CGTGCTTTATCTGATGTCGATATTGCAGTTATTAATAACGGTGTAGCAACTAAAGCGGGT
  AAAGATCCTAAAAATGATCCGATATTTTTAGAAAAATCAAATTCAGATGCAGTAAAGCCA
  TATATTAATATTGTTGCAGTTAATGACAAAGACTTGGATAACAAAACATATGCTAAAATC
  GTAGAATTGTATCATTCAAAAGAAGCTCAAAAAGCGTTGCAGGAAGATGTCAAAGATGGA
  GAGAAACCTGTTAATTTATCTAAAGATGAGATTAAGGCAATAGAAACGTCATTAGCAAAA
  TAA
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⊟Protein

⊟General

• locus tag: SAUSA300_0437 [new locus tag: SAUSA300_RS02340 ]
• symbol: SAUSA300_0437
• description: NLPA lipoprotein
• length: 280
• theoretical pI: 9.62721
• theoretical MW: 30455.9
• GRAVY: -0.315714

⊟Function

• TIGRFAM:
  Cell structure Cell envelope Other lipoprotein, YaeC family (TIGR00363; HMM-score: 196.6)
• TheSEED  :
  • Methionine ABC transporter substrate-binding protein
  Amino Acids and Derivatives Lysine, threonine, methionine, and cysteine Methionine Biosynthesis  Methionine ABC transporter substrate-binding protein
  and 2 more
  Amino Acids and Derivatives Lysine, threonine, methionine, and cysteine Methionine Degradation  Methionine ABC transporter substrate-binding protein
  Phages, Prophages, Transposable elements, Plasmids Pathogenicity islands Staphylococcal pathogenicity islands SaPI  Methionine ABC transporter substrate-binding protein
• PFAM:
  PBP (CL0177) Lipoprotein_9; NLPA lipoprotein (PF03180; HMM-score: 288.4)
  and 1 more
  NADP_Rossmann (CL0063) Ldh_1_N; lactate/malate dehydrogenase, NAD binding domain (PF00056; HMM-score: 16.4)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic Membrane
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 9.68
  • Cellwall Score: 0.17
  • Extracellular Score: 0.16
  • Internal Helices: 0
• LocateP: Lipid anchored
  • Prediction by SwissProt Classification: Extracellular
  • Pathway Prediction: Sec-(SPII)
  • Intracellular possibility: 0
  • Signal peptide possibility: 0.5
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: LLAACGG
• SignalP: Signal peptide LIPO(Sec/SPII) length 17 aa
  • SP(Sec/SPI): 0.000502
  • TAT(Tat/SPI): 0.000079
  • LIPO(Sec/SPII): 0.999216
  • Cleavage Site: CS pos: 17-18. LAA-CG. Pr: 0.9998
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 87160788 NCBI
• RefSeq: YP_493150 NCBI
• UniProt: A0A0H2XFT9 UniProt

⊟Protein sequence

• MKRLIGLVIVALVLLAACGGNNDKKVTIGVASNDTKAWEKVKELAKKDDIDVEIKHFSDYNLPNKALNDGDIDMNAFQHFAFLDQYKKAHKGTKISALSTTVLAPLGIYSDKIKDVKKVKDGAKVVIPNDVSNQARALKLLEAAGLIKLKKDFGLAGTVKDITSNPKHLKITAVDAQQTARALSDVDIAVINNGVATKAGKDPKNDPIFLEKSNSDAVKPYINIVAVNDKDLDNKTYAKIVELYHSKEAQKALQEDVKDGEKPVNLSKDEIKAIETSLAK

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: SAUSA300_0435 > SAUSA300_0436 > SAUSA300_0437

⊟Regulation

• regulator: CymR* (repression) regulon

  CymR*

  (TF)

  important in Cysteine metabolism; RegPrecise    transcription unit transferred from N315 data RegPrecise

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications

Dmitry A Rodionov, Alexey G Vitreschak, Andrey A Mironov, Mikhail S Gelfand
Comparative genomics of the methionine metabolism in Gram-positive bacteria: a variety of regulatory systems.
Nucleic Acids Res: 2004, 32(11);3340-53
[PubMed:15215334] [WorldCat.org] [DOI] (I e)Olga Soutourina, Sarah Dubrac, Olivier Poupel, Tarek Msadek, Isabelle Martin-Verstraete
The pleiotropic CymR regulator of Staphylococcus aureus plays an important role in virulence and stress response.
PLoS Pathog: 2010, 6(5);e1000894
[PubMed:20485570] [WorldCat.org] [DOI] (I e)