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NCBI: 03-AUG-2016

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_00426
  • pan locus tag?: SAUPAN002178000
  • symbol: SAOUHSC_00426
  • pan gene symbol?: metQ2
  • synonym:
  • product: ABC transporter substrate-binding protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAOUHSC_00426
  • symbol: SAOUHSC_00426
  • product: ABC transporter substrate-binding protein
  • replicon: chromosome
  • strand: +
  • coordinates: 426435..427277
  • length: 843
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    ATGAAAAGATTGATTGGGTTAGTTATCGTAGCACTTGTATTATTAGCAGCGTGTGGTGGT
    AACAATGATAAAAAAGTAACAATTGGTGTCGCATCAAATGACACTAAGGCTTGGGAGAAG
    GTTAAAGAATTAGCTAAAAAAGATGATATTGATGTGGAGATTAAGCACTTCTCAGATTAC
    AATTTACCGAATAAAGCATTAAATGATGGTGATATTGATATGAATGCATTCCAACATTTT
    GCATTTTTAGATCAATATAAAAAGGCGCATAAAGGAACAAAGATTTCAGCATTAAGTACA
    ACAGTTTTAGCACCGTTGGGCATTTACTCAGATAAAATTAAAGATGTCAAAAAGGTTAAA
    GATGGTGCTAAAGTTGTCATTCCAAATGATGTGTCAAACCAAGCACGTGCACTTAAACTA
    TTAGAAGCAGCTGGTTTAATAAAACTGAAAAAAGATTTCGGATTAGCAGGCACGGTGAAA
    GATATAACGTCAAATCCAAAACATTTAAAAATTACTGCAGTAGATGCACAACAAACTGCA
    CGTGCTTTATCTGATGTCGATATTGCAGTTATTAATAACGGTGTAGCAACTAAAGCGGGT
    AAAGATCCTAAAAATGATCCGATATTTTTAGAAAAATCAAATTCAGATGCAGTAAAGCCA
    TATATTAATATTGTTGCAGTTAATGACAAAGACTTGGATAACAAAACATATGCTAAAATC
    GTAGAATTGTATCATTCAAAAGAAGCTCAAAAAGCGTTGCAGGAAGATGTCAAAGATGGA
    GAGAAACCTGTTAATTTATCTAAAGATGAGATTAAGGCAATAGAAACGTCATTAGCAAAA
    TAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    843

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAOUHSC_00426
  • symbol: SAOUHSC_00426
  • description: ABC transporter substrate-binding protein
  • length: 280
  • theoretical pI: 9.62721
  • theoretical MW: 30455.9
  • GRAVY: -0.315714

⊟Function[edit | edit source]

  • TIGRFAM:
    Cell structure Cell envelope Other lipoprotein, YaeC family (TIGR00363; HMM-score: 196.6)
  • TheSEED  :
    • Methionine ABC transporter substrate-binding protein
    Amino Acids and Derivatives Lysine, threonine, methionine, and cysteine Methionine Biosynthesis  Methionine ABC transporter substrate-binding protein
    and 2 more
    Amino Acids and Derivatives Lysine, threonine, methionine, and cysteine Methionine Degradation  Methionine ABC transporter substrate-binding protein
    Phages, Prophages, Transposable elements, Plasmids Pathogenicity islands Staphylococcal pathogenicity islands SaPI  Methionine ABC transporter substrate-binding protein
  • PFAM:
    PBP (CL0177) Lipoprotein_9; NLPA lipoprotein (PF03180; HMM-score: 288.4)
    and 1 more
    NADP_Rossmann (CL0063) Ldh_1_N; lactate/malate dehydrogenase, NAD binding domain (PF00056; HMM-score: 16.4)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 9.68
    • Cellwall Score: 0.17
    • Extracellular Score: 0.16
    • Internal Helices: 0
  • LocateP: Lipid anchored
    • Prediction by SwissProt Classification: Extracellular
    • Pathway Prediction: Sec-(SPII)
    • Intracellular possibility: 0
    • Signal peptide possibility: 0.5
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: LLAACGG
  • SignalP: Signal peptide LIPO(Sec/SPII) length 17 aa
    • SP(Sec/SPI): 0.000502
    • TAT(Tat/SPI): 0.000079
    • LIPO(Sec/SPII): 0.999216
    • Cleavage Site: CS pos: 17-18. LAA-CG. Pr: 0.9998
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MKRLIGLVIVALVLLAACGGNNDKKVTIGVASNDTKAWEKVKELAKKDDIDVEIKHFSDYNLPNKALNDGDIDMNAFQHFAFLDQYKKAHKGTKISALSTTVLAPLGIYSDKIKDVKKVKDGAKVVIPNDVSNQARALKLLEAAGLIKLKKDFGLAGTVKDITSNPKHLKITAVDAQQTARALSDVDIAVINNGVATKAGKDPKNDPIFLEKSNSDAVKPYINIVAVNDKDLDNKTYAKIVELYHSKEAQKALQEDVKDGEKPVNLSKDEIKAIETSLAK

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas [1] [2]
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: CymR* (repression) regulon
    CymR*(TF)important in Cysteine metabolism; RegPrecise    transcription unit transferred from N315 data RegPrecise 

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-61
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  2. ↑ Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  3. ↑ 3.0 3.1 Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

Dmitry A Rodionov, Alexey G Vitreschak, Andrey A Mironov, Mikhail S Gelfand
Comparative genomics of the methionine metabolism in Gram-positive bacteria: a variety of regulatory systems.
Nucleic Acids Res: 2004, 32(11);3340-53
[PubMed:15215334] [WorldCat.org] [DOI] (I e)Olga Soutourina, Sarah Dubrac, Olivier Poupel, Tarek Msadek, Isabelle Martin-Verstraete
The pleiotropic CymR regulator of Staphylococcus aureus plays an important role in virulence and stress response.
PLoS Pathog: 2010, 6(5);e1000894
[PubMed:20485570] [WorldCat.org] [DOI] (I e)

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