NCBI: 10-JUN-2013
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus COL
- locus tag: SACOL1057 [new locus tag: SACOL_RS05390 ]
- pan locus tag?: SAUPAN003251000
- symbol: sspA
- pan gene symbol?: sspA
- synonym:
- product: V8 protease
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: SACOL1057 [new locus tag: SACOL_RS05390 ]
- symbol: sspA
- product: V8 protease
- replicon: chromosome
- strand: -
- coordinates: 1063016..1064026
- length: 1011
- essential: unknown other strains
⊟Accession numbers[edit | edit source]
- Gene ID: 3237584 NCBI
- RefSeq: YP_185922 NCBI
- BioCyc: see SACOL_RS05390
- MicrobesOnline: 912526 MicrobesOnline
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
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961ATGAAAGGTAAATTTTTAAAAGTTAGTTCTTTATTCGTTGCAACTTTGACAACAGCGACA
CTTGTGAGTTCTCCAGCAGCAAACGCGTTATCTTCAAAGGCTATGGACAATCATCCACAA
CAAACGCAGTCAAGCAAACAGCAAACACCTAAGATTCAAAAAGGCGGTAACCTTAAACCA
TTAGAACAACGTGAACACGCAAATGTTATATTACCAAATAACGATCGTCACCAAATCACA
GATACAACGAATGGTCATTATGCACCCGTAACTTATATTCAAGTTGAAGCACCTACTGGT
ACATTTATTGCTTCCGGTGTAGTTGTAGGTAAAGATACTCTTTTAACAAATAAACACGTC
GTAGATGCTACGCACGGTGATCCTCATGCTTTAAAAGCATTCCCTTCTGCAATTAACCAA
GACAATTATCCAAATGGTGGTTTCACTGCTGAACAAATCACTAAATATTCAGGCGAAGGT
GATTTAGCAATAGTTAAATTCTCCCCTAATGAGCAAAACAAACATATTGGTGAAGTAGTT
AAACCAGCAACAATGAGTAATAATGCTGAAACACAAGTTAACCAAAATATTACTGTAACA
GGATATCCTGGTGATAAACCTGTAGCAACAATGTGGGAAAGTAAAGGAAAAATCACTTAC
CTCAAAGGCGAAGCTATGCAATATGATTTAAGTACAACTGGTGGTAATTCAGGTTCACCT
GTATTTAATGAAAAAAATGAAGTGATCGGAATTCATTGGGGCGGTGTACCAAATGAATTT
AATGGTGCGGTATTTATTAATGAAAATGTACGCAACTTCTTAAAACAAAATATTGAAGAT
ATCCATTTTGCCAACGATGACCAACCTAATAACCCAGATAATCCTGATAACCCTAACAAT
CCTGATAACCCTAACAACCCAGATGAACCAAATAACCCTGACAACCCTAACAACCCTGAT
AATCCAGACAATGGCGATACCAATAATTCAGACAATCCAGATGCAGCTTAA60
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⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: SACOL1057 [new locus tag: SACOL_RS05390 ]
- symbol: SspA
- description: V8 protease
- length: 336
- theoretical pI: 4.80889
- theoretical MW: 36312.4
- GRAVY: -0.831548
⊟Function[edit | edit source]
- ⊞reaction: EC 3.4.21.19? ExPASy
- ⊞TIGRFAM: Protein fate Degradation of proteins, peptides, and glycopeptides peptidase Do (TIGR02037; EC 3.4.21.-; HMM-score: 18.1)Protein fate Protein folding and stabilization peptidase Do (TIGR02037; EC 3.4.21.-; HMM-score: 18.1)and 2 more
- TheSEED :
- Glutamyl endopeptidase precursor (EC 3.4.21.19), serine proteinase SspA
- ⊞PFAM: Peptidase_PA (CL0124) Trypsin_2; Trypsin-like peptidase domain (PF13365; HMM-score: 75.1)and 7 more
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
⊟Accession numbers[edit | edit source]
⊟Protein sequence[edit | edit source]
- MKGKFLKVSSLFVATLTTATLVSSPAANALSSKAMDNHPQQTQSSKQQTPKIQKGGNLKPLEQREHANVILPNNDRHQITDTTNGHYAPVTYIQVEAPTGTFIASGVVVGKDTLLTNKHVVDATHGDPHALKAFPSAINQDNYPNGGFTAEQITKYSGEGDLAIVKFSPNEQNKHIGEVVKPATMSNNAETQVNQNITVTGYPGDKPVATMWESKGKITYLKGEAMQYDLSTTGGNSGSPVFNEKNEVIGIHWGGVPNEFNGAVFINENVRNFLKQNIEDIHFANDDQPNNPDNPDNPNNPDNPNNPDEPNNPDNPNNPDNPDNGDTNNSDNPDAA
⊟Experimental data[edit | edit source]
- experimentally validated: PeptideAtlas
- protein localization:
- quantitative data / protein copy number per cell:
- interaction partners:
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
- MicrobesOnline: no polycistronic organisation predicted
⊟Regulation[edit | edit source]
- regulator:
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: data available for NCTC8325
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
- Aureolib: no data available
⊟Protein stability[edit | edit source]
- half-life: no data available
⊞Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊞Other Information[edit | edit source]
⊟Literature[edit | edit source]
⊟References[edit | edit source]
⊟Relevant publications[edit | edit source]
Yusuke Hirasawa, Toshiro Takai, Toshinobu Nakamura, Kouichi Mitsuishi, Hendra Gunawan, Hajime Suto, Takasuke Ogawa, Xiao-Ling Wang, Shigaku Ikeda, Ko Okumura, Hideoki Ogawa
Staphylococcus aureus extracellular protease causes epidermal barrier dysfunction.
J Invest Dermatol: 2010, 130(2);614-7
[PubMed:19812593] [WorldCat.org] [DOI] (I p)