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NCBI: 06-JUL-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_0049 [new locus tag: NWMN_RS00285 ]
  • pan locus tag?: SAUPAN000899000
  • symbol: NWMN_0049
  • pan gene symbol?:
  • synonym:
  • product: hypothetical protein

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: NWMN_0049 [new locus tag: NWMN_RS00285 ]
  • symbol: NWMN_0049
  • product: hypothetical protein
  • replicon: chromosome
  • strand: -
  • coordinates: 64264..65931
  • length: 1668
  • essential: unknown other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    ATGGAAATGTCGGTTACAGAAGTCATTTTCTCCTTTTTAGGTGGTTTAGGTATTTTCCTT
    TACGGCTTAAAAATCATGGGAGACGGGCTTCAAGCATCAGCAGGAGACAGGCTACGAGAT
    ATTTTAAACAAATTTACATCAAATCCAGTATTAGGTGTTATTGCAGGTATCGTTGTAACT
    ATTTTAATACAAAGTAGTTCAGGTACGACAGTTATCACAATCGGACTGGTAACAGCTGGA
    TTTATGACATTGAAACAAGCCATTGGAGTGATAATGGGTGCTAATATCGGAACAACGGTA
    ACTGCATTTATTATCGGTATAGATTTAGGCGAATATGCAATGCCAATTTTAGCATTAGGT
    GCATTCTTAATCTTTTTCTTTAAACGCTCTAAAATCAATAACATTGGCCGCATACTATTC
    GGTTTCGGTTCACTATTCTTCGGTCTAGAATTTATGGGTGATGCCGTTAAACCTTTAGCA
    TCATTAGATGGATTTAAGCAATTAATGCTTGATATGTCTACAAATCCAATACTCGCTGTC
    ATTGTCGGCGCAGGGTTAACAGCACTAGTTCAAAGTTCAAGTGCGACGATTGGTATTTTA
    CAAGAATTTTATCAACAAGATTTAATTAGCTTAAACGCAGCAATCCCTGTGTTACTAGGC
    GATAACATTGGTACCACGATTACAGCTATCTTAGCTAGTTTAGCCGGCTCAATCGCTGCA
    AAACGTGCGGCGCTTGTACACGTCATCTTTAACTTAATCGGGGTAATTATCTTCACAATT
    TTCTTGCCAGTTGTGATTCATTTGATTAGTTTGTTACAAGATTTATGGCACTTAAAACCA
    GCGATGACGATTGCAGTATCACATGGTATCTTCAACATAACAAATACTTTGATTCAATTA
    CCATTTGTAGCAGGTTTAGCATGGATTGTTACAAAGCTTGTCCCAGGTAAAGATATTGCT
    GATGACTATAAACCTCAGCACTTAAACAAAGATCTTGTTTATCACGCACCTGGTGTTGCA
    TTACAAGAAACTCAAAAAGAATTACAAAATGTGGGTCAGATTGTCTTATCAATGTTTGAA
    GACATTCGCGAAATTACAAAAGACGATAAAAAATTGATCAAAAAGCTTGAACAAAAGCAT
    CAAGCTGTTGAAACAATCAATGATAGCATTCGAAATTATTTAGTTAGAATTTCTACAAAA
    GCCATTACGAAGGCAGACGTTGAGCGTTTAGCAGTTATGTTTGATGTCAATCGCTCTATT
    TTAAAAGTAGCAGAGCTAACAGAAGAGTATGTCGCTCAATTAAAACGCCAACATGATGAA
    GATATTCGCATTACAGAAGATGCACAACGCGGTATGGATAAATTATTCAACCATGTTGCT
    GAGTCATTTGATAAAGCCATCGACATGTTAGATGTTTATGACAAAACGAAAAAAGATGAA
    ATTGTAGAACGTAGTAGAGAATCATTTAATATTGAACATAAACTACGCAAAGGTCATATT
    AAACGCCTTAATCGTGGTGAATGTACAACAAAAGGCGGATTACTATATATCGATATGATT
    GGTGTTCTTGAACGTATCGGTTATCATTCACGAAATGTTTCTGAAGCACTTGTTGGCCTT
    AACGATGATGTACCTACAGATGAAGAAATTGCAACAACTGAAATTTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1668

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: NWMN_0049 [new locus tag: NWMN_RS00285 ]
  • symbol: NWMN_0049
  • description: hypothetical protein
  • length: 555
  • theoretical pI: 6.25194
  • theoretical MW: 60907.8
  • GRAVY: 0.329369

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    MetabolismTransport and binding proteinsCations and iron carrying compoundsNa/Pi-cotransporter II-related protein (TIGR00704; HMM-score: 250.1)
    MetabolismTransport and binding proteinsCations and iron carrying compoundssodium-dependent inorganic phosphate (Pi) transporter (TIGR01013; HMM-score: 121.5)
    MetabolismTransport and binding proteinsAnionsphosphate transport system regulatory protein PhoU (TIGR02135; HMM-score: 34.8)
    Signal transductionRegulatory functionsOtherphosphate transport system regulatory protein PhoU (TIGR02135; HMM-score: 34.8)
  • TheSEED:  
    Phosphorus MetabolismPhosphorus Metabolism - no subcategoryPhosphate metabolism Na/Pi cotransporter II-related protein 
  • PFAM:
    no clan definedNa_Pi_cotrans; Na+/Pi-cotransporter (PF02690; HMM-score: 234.3)
    PhoU (CL0297) PhoU; PhoU domain (PF01895; HMM-score: 49.8)
    no clan definedApoO; Apolipoprotein O (PF09769; HMM-score: 13.8)
    Mis12; Mis12 protein (PF05859; HMM-score: 13.4)
    IBP39; Initiator binding protein 39 kDa (PF11422; HMM-score: 11.8)
    HUP (CL0039) tRNA-synt_1e; tRNA synthetases class I (C) catalytic domain (PF01406; HMM-score: 11.7)
    no clan definedMutS_III; MutS domain III (PF05192; HMM-score: 11.1)
    KxDL; Uncharacterized conserved protein (PF10241; HMM-score: 8.3)
    Med30; Mediator complex subunit 30 (PF11315; HMM-score: 7.9)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 9
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular Possibility: 0.17
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.143
    • Ymax_pos: 35
    • Cmax: 0.135
    • Cmax_pos: 35
    • Smax: 0.193
    • Smax_pos: 27
    • Smean: 0.107
    • D: 0.129
  • predicted transmembrane helices (TMHMM): 9

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MEMSVTEVIFSFLGGLGIFLYGLKIMGDGLQASAGDRLRDILNKFTSNPVLGVIAGIVVTILIQSSSGTTVITIGLVTAGFMTLKQAIGVIMGANIGTTVTAFIIGIDLGEYAMPILALGAFLIFFFKRSKINNIGRILFGFGSLFFGLEFMGDAVKPLASLDGFKQLMLDMSTNPILAVIVGAGLTALVQSSSATIGILQEFYQQDLISLNAAIPVLLGDNIGTTITAILASLAGSIAAKRAALVHVIFNLIGVIIFTIFLPVVIHLISLLQDLWHLKPAMTIAVSHGIFNITNTLIQLPFVAGLAWIVTKLVPGKDIADDYKPQHLNKDLVYHAPGVALQETQKELQNVGQIVLSMFEDIREITKDDKKLIKKLEQKHQAVETINDSIRNYLVRISTKAITKADVERLAVMFDVNRSILKVAELTEEYVAQLKRQHDEDIRITEDAQRGMDKLFNHVAESFDKAIDMLDVYDKTKKDEIVERSRESFNIEHKLRKGHIKRLNRGECTTKGGLLYIDMIGVLERIGYHSRNVSEALVGLNDDVPTDEEIATTEI

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]