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NCBI: 06-JUL-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_0065 [new locus tag: NWMN_RS00365 ]
  • pan locus tag?: SAUPAN000920000
  • symbol: sbnF
  • pan gene symbol?: sbnF
  • synonym:
  • product: siderophore biosynthesis IucC family protein

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: NWMN_0065 [new locus tag: NWMN_RS00365 ]
  • symbol: sbnF
  • product: siderophore biosynthesis IucC family protein
  • replicon: chromosome
  • strand: +
  • coordinates: 86371..88221
  • length: 1851
  • essential: unknown other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGATTGTGGTGCAAACCCTATTTATACACATATACCAAATCCAATTTGTCATAACAAGG
    AGGTATCGTATTGTGAATCAAACAATTCTTAATCGTGTAAAGACTAGAGTGATGCACCAA
    CTGGTATCATCACTTATTTATGAGAATATTGTTGTGTATAAAGCGTCATATCAAGACGGT
    GTCGGTCATTTTACAATAGAAGGACATGATTCAGAGTATCGTTTTACTGCTGAAAAGACA
    CATAGCTTTGATCGTATACGTATCACATCACCAATTGAGCGTGTCGTAGGAGATGAGGCA
    GATACAACAACAGACTATACACAATTATTGAGAGAGGTTGTATTTACATTTCCTAAAAAT
    GATGAAAAGCTAGAACAATTTATTGTCGAGTTATTACAGACAGAATTAAAAGATACGCAA
    AGTATGCAGTATCGAGAATCAAACCCACCAGCAACACCTGAGACATTTAACGACTATGAA
    TTTTATGCGATGGAAGGGCATCAGTATCATCCAAGTTACAAATCACGTTTAGGATTTACG
    TTGAGTGATAATTTGAAATTTGGTCCTGATTTTGTACCAAACGTTAAACTGCAGTGGTTA
    GCTATCGACAAAGATAAAGTAGAAACGACGGTATCAAGAAATGTTGTAGTTAACGAAATG
    TTACGTCAACAAGTTGGCGATAAGACTTATGAACATTTTGTACAGCAAATTGAAGCATCT
    GGCAAACATGTAAATGATGTTGAGATGATACCTGTACACCCATGGCAGTTTGAACATGTC
    ATCCAAGTTGATTTGGCTGAAGAAAGGCTTAATGGCACAGTACTATGGTTAGGGGAAAGT
    GATGAGCTATATCACCCTCAACAATCGATTCGTACGATGTCGCCAATAGACACGACAAAA
    TATTATTTAAAGGTACCAATAAGTATAACGAACACTTCAACGAAACGAGTGTTGGCGCCT
    CATACAATTGAAAATGCAGCGCAAATTACGGATTGGTTAAAGCAGATACAGCAACAAGAT
    ATGTATTTAAAAGATGAATTAAAGACAGTTTTTCTAGGGGAAGTCTTAGGACAGTCTTAT
    TTAAATACACAACTTTCGCCTTATAAACAAACTCAAGTTTATGGTGCGTTAGGTGTTATA
    TGGCGTGAAAATATATATCATATGTTAATCGATGAAGAGGATGCGATACCATTTAATGCA
    CTTTATGCAAGTGATAAGGATGGTGTACCATTCATTGAAAATTGGATTAAACAATATGGT
    TCTGAAGCTTGGACAAAGCAATTTTTAGCTGTAGCGATTCGTCCAATGATTCATATGCTT
    TATTATCACGGTATTGCCTTTGAATCACATGCACAAAATATGATGCTCATTCATGAAAAT
    GGTTGGCCTACACGTATTGCCTTAAAAGATTTCCATGATGGTGTTCGTTTTAAGCGTGAG
    CATTTAAGTGAAGCTGCTTCACACCTGACATTAAAGCCAATGCCAGAAGCACATAAAAAA
    GTGAATAGTAATTCATTTATTGAAACAGATGACGAACGTTTAGTACGCGACTTTTTACAT
    GATGCATTTTTCTTTATTAATATCGCCGAAATCATCTTATTTATTGAAAAGCAATATGGT
    ATCGATGAGGAGCTGCAATGGCAATGGGTTAAAGGCATCATCGAGGCGTATCAAGAAGCA
    TTTCCAGAGTTGAATAACTATCAACATTTCGATTTGTTTGAACCTACGATTCAAGTTGAA
    AAGTTAACGACACGTCGATTATTAAGTGACTCCGAGTTAAGAATTCATCATGTTACAAAT
    CCATTAGGTGTAGGAGGTATCAATGATGCAACAACTATCTCTGAAACATAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1740
    1800
    1851

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: NWMN_0065 [new locus tag: NWMN_RS00365 ]
  • symbol: SbnF
  • description: siderophore biosynthesis IucC family protein
  • length: 616
  • theoretical pI: 5.36729
  • theoretical MW: 71951
  • GRAVY: -0.385227

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
  • TheSEED:  
    Iron acquisition and metabolismSiderophoresSiderophore Staphylobactin Siderophore staphylobactin biosynthesis protein SbnF 
    Siderophore Staphylobactin Siderophore synthetase superfamily, group C 
  • PFAM:
    PKinase (CL0016) IucA_IucC; IucA / IucC family (PF04183; HMM-score: 215.5)
    no clan definedFhuF; Ferric iron reductase FhuF-like transporter (PF06276; HMM-score: 125.7)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.115
    • Ymax_pos: 39
    • Cmax: 0.125
    • Cmax_pos: 55
    • Smax: 0.175
    • Smax_pos: 52
    • Smean: 0.093
    • D: 0.106
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MIVVQTLFIHIYQIQFVITRRYRIVNQTILNRVKTRVMHQLVSSLIYENIVVYKASYQDGVGHFTIEGHDSEYRFTAEKTHSFDRIRITSPIERVVGDEADTTTDYTQLLREVVFTFPKNDEKLEQFIVELLQTELKDTQSMQYRESNPPATPETFNDYEFYAMEGHQYHPSYKSRLGFTLSDNLKFGPDFVPNVKLQWLAIDKDKVETTVSRNVVVNEMLRQQVGDKTYEHFVQQIEASGKHVNDVEMIPVHPWQFEHVIQVDLAEERLNGTVLWLGESDELYHPQQSIRTMSPIDTTKYYLKVPISITNTSTKRVLAPHTIENAAQITDWLKQIQQQDMYLKDELKTVFLGEVLGQSYLNTQLSPYKQTQVYGALGVIWRENIYHMLIDEEDAIPFNALYASDKDGVPFIENWIKQYGSEAWTKQFLAVAIRPMIHMLYYHGIAFESHAQNMMLIHENGWPTRIALKDFHDGVRFKREHLSEAASHLTLKPMPEAHKKVNSNSFIETDDERLVRDFLHDAFFFINIAEIILFIEKQYGIDEELQWQWVKGIIEAYQEAFPELNNYQHFDLFEPTIQVEKLTTRRLLSDSELRIHHVTNPLGVGGINDATTISET

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator: NWMN_1406 (Fur*)
    Fur* (TF) important in Iron homeostasisRegPrecise    transcription unit transferred from N315 data RegPrecise

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]