From AureoWiki
Jump to: navigation, search
NCBI: 06-JUL-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_0114 [new locus tag: NWMN_RS00630 ]
  • pan locus tag?: SAUPAN001005000
  • symbol: NWMN_0114
  • pan gene symbol?:
  • synonym:
  • product: cation efflux family protein

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: NWMN_0114 [new locus tag: NWMN_RS00630 ]
  • symbol: NWMN_0114
  • product: cation efflux family protein
  • replicon: chromosome
  • strand: +
  • coordinates: 139273..140337
  • length: 1065
  • essential: unknown other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    ATGGTTAATTACGGAAGCATTAATATTAACCTGAGAAGCTATAAAGAATTATTTTTAAAA
    GCGACAATATTAAATACGACGCATTTATTTAGGAGTGGCAAACGTATGAATGGGAAAAAG
    GCGAATACGATAAACAGATACAAATATTTTCATCATGTCAATCATCAAAAAATTCAACAA
    AGTTCTAAAAAGACGCTGTGGGCATCACTAATCATCACATTGTTATTTACAGTGATTGAA
    TTTGTCGGAGGTTTAGTATCTAATTCATTGGCATTACTGTCAGATTCATTTCATATGCTT
    AGTGATGTATTAGCACTTGGTTTATCTATGTTGGCCATTTATTTTGCAAGTAAAAAGCCG
    ACTGCACGATACACATTTGGATATTTAAGATTTGAGATATTAGCTGCATTTTTAAATGGT
    TTAGCATTAATTGTAATTTCAATCTGGATTTTATATGAAGCTATTGTACGTATTATTTAT
    CCGCAACCAATTGAAAGTGGCATTATGTTTATGATTGCTAGTATTGGTTTACTCGTCAAT
    ATTATTTTGACTGTTATCCTTGTAAGGTCTTTAAAACAAGAAGACAATATCAATATTCAA
    AGTGCATTATGGCATTTCATGGGAGACTTATTGAACTCTATTGGTGTCATCGTTGCAGTT
    GTATTGATTTACTTTACAGGATGGCGCATCATCGACCCAATCATTAGTATTGTAATTTCA
    CTCATCATTTTACGTGGTGGTTATAAAATTACGCGTAATGCGTGGTTAATTTTAATGGAA
    AGTGTGCCTCAACATTTGGATACTGATCAAATTATGGCAGATATTAAAAACATAGATGGC
    ATATTAGATGTACATGAATTTCATTTGTGGAGTATTACAACAGAGCATTATTCATTAAGT
    GCCCATGTTGTGTTAGATAAAAAATATGAGGGTGATGATTATCAAGCGATTGATCAAGTA
    TCATCATTGTTGAAAGAAAAATATGGCATTGCACATTCAACGTTGCAAATTGAAAACTTG
    CAATTGAATCCATTAGATGAGCCATACTTCGACAAATTAACATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1065

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: NWMN_0114 [new locus tag: NWMN_RS00630 ]
  • symbol: NWMN_0114
  • description: cation efflux family protein
  • length: 354
  • theoretical pI: 8.59269
  • theoretical MW: 40275
  • GRAVY: 0.377401

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    MetabolismTransport and binding proteinsCations and iron carrying compoundscation diffusion facilitator family transporter (TIGR01297; HMM-score: 307.1)
    Cellular processesCellular processesSporulation and germinationstage III sporulation protein AC (TIGR02848; HMM-score: 10)
  • TheSEED:  
    Potassium metabolismPotassium metabolism - no subcategoryGlutathione-regulated potassium-efflux system and associated functions Cobalt-zinc-cadmium resistance protein 
    Virulence, Disease and DefenseResistance to antibiotics and toxic compoundsCobalt-zinc-cadmium resistance Cobalt-zinc-cadmium resistance protein 
  • PFAM:
    no clan definedCation_efflux; Cation efflux family (PF01545; HMM-score: 174.7)
    DUF2663; Protein of unknown function (DUF2663) (PF10864; HMM-score: 12.9)
    Transporter (CL0375) SUR7; SUR7/PalI family (PF06687; HMM-score: 8.9)
    no clan definedDUF2070; Predicted membrane protein (DUF2070) (PF09843; HMM-score: 8.3)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 6
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular Possibility: 0.17
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.153
    • Ymax_pos: 21
    • Cmax: 0.115
    • Cmax_pos: 42
    • Smax: 0.308
    • Smax_pos: 11
    • Smean: 0.206
    • D: 0.174
  • predicted transmembrane helices (TMHMM): 6

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MVNYGSININLRSYKELFLKATILNTTHLFRSGKRMNGKKANTINRYKYFHHVNHQKIQQSSKKTLWASLIITLLFTVIEFVGGLVSNSLALLSDSFHMLSDVLALGLSMLAIYFASKKPTARYTFGYLRFEILAAFLNGLALIVISIWILYEAIVRIIYPQPIESGIMFMIASIGLLVNIILTVILVRSLKQEDNINIQSALWHFMGDLLNSIGVIVAVVLIYFTGWRIIDPIISIVISLIILRGGYKITRNAWLILMESVPQHLDTDQIMADIKNIDGILDVHEFHLWSITTEHYSLSAHVVLDKKYEGDDYQAIDQVSSLLKEKYGIAHSTLQIENLQLNPLDEPYFDKLT

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor: SigB [1] other strains
    SigB (sigma factor) controls a large regulon involved in stress/starvation response and adaptation
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Markus Bischoff, Paul Dunman, Jan Kormanec, Daphne Macapagal, Ellen Murphy, William Mounts, Brigitte Berger-Bächi, Steven Projan
    Microarray-based analysis of the Staphylococcus aureus sigmaB regulon.
    J. Bacteriol.: 2004, 186(13);4085-99
    [PubMed:15205410] [WorldCat.org] [DOI] (P p)

Relevant publications[edit source | edit]