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NCBI: 06-JUL-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_0439 [new locus tag: NWMN_RS02480 ]
  • pan locus tag?: SAUPAN002191000
  • symbol: treC
  • pan gene symbol?: treC
  • synonym:
  • product: alpha,alpha-phosphotrehalase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: NWMN_0439 [new locus tag: NWMN_RS02480 ]
  • symbol: treC
  • product: alpha,alpha-phosphotrehalase
  • replicon: chromosome
  • strand: +
  • coordinates: 495300..496940
  • length: 1641
  • essential: unknown other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    GTGTCGAAAGAAATAGATTGGAGAAAATCCGTTGTATATCAAATTTATCCTAAGTCGTTT
    AATGATACGACGGGGAATGGTATAGGAGATATCAATGGAATTATAGAAAAATTGGATTAT
    ATCAAGTTATTGGGTGTTGATTATATTTGGTTAACACCAGTGTATGAATCACCGATGAAT
    GATAATGGCTATGATATCAGCAATTATTTAGAAATCAATGAAGACTTTGGAACGATGGAT
    GATTTTGAAAAGTTAATCAAAGTTGCGCATCAAAAAGACTTGAAAGTGATGTTAGATATT
    GTCATTAATCATACGTCGACGGAGCATGAATGGTTTAAAGAAGCCCGTAAATCTAAAGAT
    AACCCTTATAGAGATTATTACTTTTTCAGATCATCTGAAGACGGGCCGCCAACAAATTGG
    CATTCTAAATTCGGTGGTAATGCATGGAAGTATGATTCTGAGACAGATGAATATTATTTA
    CATTTATTTGATGTCAGTCAAGCTGATTTAAATTGGGATAATCCGGAAGTACGTCAATCG
    TTATATCGCATAGTCAATCATTGGATAGACTTCGGCGTTGATGGTTTTCGATTTGATGTC
    ATTAACTTAATTTCTAAAGGTGAATTTAAGGACTCTGACAAAATAGGTAAAGAATTTTAT
    ACGGATGGTCCTAGAGTGCATGAGTTTCTGCATGAATTAAATCGTCAAACGTTTGGTAAC
    ACTGACATGATGACTATAGGAGAAATGTCTTCGACGACGATTGAAAATTGTATTAAGTAT
    ACACAACCAGAACGCCAAGAATTGAATAGTGTTTTTAATTTTCATCATCTAAAGGTTGAT
    TATGTTGATGGTGAAAAGTGGACAAATGCGAAGCTTGATTTTCATAAGTTAAAGGAAATT
    CTGATGCAATGGCAACGAGGTATTTATGACGGTGGCGGATGGAACGCGATTTTCTGGTGT
    AATCATGATCAGCCACGGGTAGTGTCTAGATTTGGTGATGATACGTCGGAAGAGATGAGG
    ATACAAAGTGCTAAAATGTTAGCTATCGCACTGCATATGTTGCAAGGGACGCCATATATT
    TACCAAGGTGAAGAAATTGGTATGACGGACCCACATTTTACATCAATAGCACAATATCGT
    GATGTTGAATCGATTAATGCCTACCATCAGTTGTTAAGTGAAGGGCATGCTGAAGCGGAT
    GTGTTAGCGATTTTAGGACAGAAGTCACGAGACAATTCGAGAACGCCTATGCAATGGAGT
    GATGATGTTAATGCTGGATTTACAGCTGGTAAGCCTTGGATTGATATTTCGGAAAATTAT
    CATCAGGTCAACGTTAGACAAGCACTTCAGAATAAAGAGTCTATTTTCTATACGTATCAA
    AAATTAATACAATTAAGACATACGCATGATATTATTACGTATGGAGACATTGTGCCACGT
    TTTATGGATCATGATCATTTATTTGTTTATGAACGTCATTATAAGAATCAACAATGGCTA
    GTAATTGCGAATTTCTCAGCATCGGCTGTTGATTTGCCAGAAGGATTGGCTAGAGAAGGT
    TGTGTTGTGATTCAAACAGGCACAGTGGAAAATAATACGATAAGCGGGTTTGGTGCAATT
    GTAATCGAAACAAACGCGTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1641

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: NWMN_0439 [new locus tag: NWMN_RS02480 ]
  • symbol: TreC
  • description: alpha,alpha-phosphotrehalase
  • length: 546
  • theoretical pI: 4.88774
  • theoretical MW: 63513.4
  • GRAVY: -0.575641

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    alpha,alpha-phosphotrehalase (TIGR02403; EC 3.2.1.93; HMM-score: 816.1)
    MetabolismEnergy metabolismBiosynthesis and degradation of polysaccharidestrehalose synthase (TIGR02456; EC 5.4.99.16; HMM-score: 295.6)
    MetabolismEnergy metabolismBiosynthesis and degradation of polysaccharidesmalto-oligosyltrehalose trehalohydrolase (TIGR02402; EC 3.2.1.141; HMM-score: 81.6)
    MetabolismEnergy metabolismBiosynthesis and degradation of polysaccharidesmalto-oligosyltrehalose synthase (TIGR02401; EC 5.4.99.15; HMM-score: 58.3)
    MetabolismEnergy metabolismBiosynthesis and degradation of polysaccharides1,4-alpha-glucan branching enzyme (TIGR01515; EC 2.4.1.18; HMM-score: 37.5)
    MetabolismEnergy metabolismBiosynthesis and degradation of polysaccharidesglycogen debranching enzyme GlgX (TIGR02100; EC 3.2.1.-; HMM-score: 34.9)
    sucrose phosphorylase (TIGR03852; EC 2.4.1.7; HMM-score: 32)
    pullulanase, type I (TIGR02104; EC 3.2.1.41; HMM-score: 31.4)
    pullulanase, extracellular (TIGR02102; HMM-score: 20.7)
    MetabolismEnergy metabolismBiosynthesis and degradation of polysaccharidesglycogen debranching enzyme (TIGR01531; HMM-score: 20.3)
  • TheSEED:  
    CarbohydratesDi- and oligosaccharidesTrehalose Uptake and Utilization Trehalose-6-phosphate hydrolase (EC 3.2.1.93) 
  • PFAM:
    Glyco_hydro_tim (CL0058) Alpha-amylase; Alpha amylase, catalytic domain (PF00128; HMM-score: 411.3)
    hDGE_amylase; Glycogen debranching enzyme, glucanotransferase domain (PF14701; HMM-score: 28.2)
    GHD (CL0369) Malt_amylase_C; Maltogenic Amylase, C-terminal domain (PF16657; HMM-score: 22.8)
    DUF3459; Domain of unknown function (DUF3459) (PF11941; HMM-score: 20.9)
    Cyc-maltodext_C; Cyclo-malto-dextrinase C-terminal domain (PF10438; HMM-score: 13.3)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.107
    • Ymax_pos: 28
    • Cmax: 0.106
    • Cmax_pos: 22
    • Smax: 0.154
    • Smax_pos: 25
    • Smean: 0.09
    • D: 0.1
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MSKEIDWRKSVVYQIYPKSFNDTTGNGIGDINGIIEKLDYIKLLGVDYIWLTPVYESPMNDNGYDISNYLEINEDFGTMDDFEKLIKVAHQKDLKVMLDIVINHTSTEHEWFKEARKSKDNPYRDYYFFRSSEDGPPTNWHSKFGGNAWKYDSETDEYYLHLFDVSQADLNWDNPEVRQSLYRIVNHWIDFGVDGFRFDVINLISKGEFKDSDKIGKEFYTDGPRVHEFLHELNRQTFGNTDMMTIGEMSSTTIENCIKYTQPERQELNSVFNFHHLKVDYVDGEKWTNAKLDFHKLKEILMQWQRGIYDGGGWNAIFWCNHDQPRVVSRFGDDTSEEMRIQSAKMLAIALHMLQGTPYIYQGEEIGMTDPHFTSIAQYRDVESINAYHQLLSEGHAEADVLAILGQKSRDNSRTPMQWSDDVNAGFTAGKPWIDISENYHQVNVRQALQNKESIFYTYQKLIQLRHTHDIITYGDIVPRFMDHDHLFVYERHYKNQQWLVIANFSASAVDLPEGLAREGCVVIQTGTVENNTISGFGAIVIETNA

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator: CcpA
    CcpA (TF) important in Carbon catabolism  RegPrecise    transcription unit transferred from N315 data RegPrecise

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]