NCBI: 06-JUL-2013

⊟Summary

• organism: Staphylococcus aureus Newman
• locus tag: NWMN_0490 [new locus tag: NWMN_RS02865 ]
• pan locus tag^?: SAUPAN002294000
• symbol: gltX
• pan gene symbol^?: gltX
• synonym:
• product: glutamyl-tRNA synthetase

⊟Gene

⊟General

• type: CDS
• locus tag: NWMN_0490 [new locus tag: NWMN_RS02865 ]
• symbol: gltX
• product: glutamyl-tRNA synthetase
• replicon: chromosome
• strand: +
• coordinates: 565103..566557
• length: 1455
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 5330260 NCBI
• RefSeq: YP_001331524 NCBI
• BioCyc:
• MicrobesOnline: 3706037 MicrobesOnline

⊟Phenotype

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⊟DNA sequence

• 1
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  121
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  481
  541
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  841
  901
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  1021
  1081
  1141
  1201
  1261
  1321
  1381
  1441
  
  ATGAGCGATCGTATAAGAGTAAGATATGCACCAAGTCCAACTGGGTATCTTCATATTGGT
  AATGCAAGAACAGCATTATTCAATTACTTGTATGCTAAACATTACAACGGAGATTTTGTG
  ATTCGAATTGAAGATACTGATAAAAAACGTAATTTAGAAGATGGAGAAACATCACAATTT
  GATAATCTTAAATGGTTAGGATTAGATTGGGATGAGTCTGTAGATAAAGACAATGGCTAC
  GGACCATATCGTCAATCTGAACGTCAACATATCTACCAACCATTAATAGATCAGTTACTA
  GCAGAAGATAAAGCATATAAATGCTATATGACAGAAGAAGAATTAGAAGCTGAACGTGAA
  GCGCAAATCGCTCGTGGTGAAATGCCTCGCTATGGTGGTCAACATGCGCATTTGACTGAA
  GAACAACGTCAACAATTTGAAGCAGAAGGACGCCAACCATCAATTCGTTTCCGAGTACCT
  CAAAACCAAACGTATTCATTTGATGATATGGTAAAAGGAAATATTTCATTTGATTCAAAT
  GGTATTGGTGACTGGGTTATCGTAAAAAAAGATGGCATTCCAACGTACAATTTTGCAGTA
  GCTATAGATGATCATTACATGCAAATTTCAGATGTAATTCGTGGTGATGATCATATTTCA
  AACACGCCTAAACAAATTATGATTTATGAAGCATTTGGCTGGGAGCCACCTCGTTTTGGT
  CATATGTCATTAATTGTTAATGAAGAACGTAAAAAGTTAAGTAAACGTGATGGGCAAATT
  TTACAATTTATTGAGCAATATCGTGACTTAGGTTATTTACCTGAAGCGTTATTTAATTTT
  ATTGCGTTATTAGGTTGGTCTCCTGAAGGTGAAGAAGAAATCTTTTCTAAAGAAGAATTT
  ATCAAAATCTTTGATGAAAAGCGTTTGTCAAAATCACCAGCATTTTTCGATAAGCAAAAA
  TTAGCATGGGTTAATAACCAATATATGAAACAAAAAGATACTGAAACAGTATTCCAATTA
  GCATTACCTCATTTAATTAAAGCAAATTTGATTCCTGAGGTGCCGTCAGAAGAGGATTTA
  TCTTGGGGACGCAAATTAATTGCGCTTTATCAAAAAGAAATGAGTTATGCCGGTGAAATT
  GTACCTTTATCAGAAATGTTCTTTAAAGAAATGCCAGCTCTTGGTGAAGAAGAACAACAA
  GTGATTAATGGAGAGCAAGTACCAGAGTTAATGACGCACTTATTCAGTAAATTAGAAGCA
  CTTGAACCATTTGAATCGGCTGAAATTAAAAAGACAATTAAAGAAGTTCAAAAAGAAACA
  GGAATAAAAGGCAAGCAATTATTTATGCCTATTCGTGTTGCTGTAACAGGCCAAATGCAT
  GGTCCTGAATTACCAAATACAATTGAAGTACTTGGTAAAGAAAAAGTGCTAAACCGTTTA
  AAACAATATAAGTAA
  60
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  1455
  

⊟Protein

⊟General

• locus tag: NWMN_0490 [new locus tag: NWMN_RS02865 ]
• symbol: GltX
• description: glutamyl-tRNA synthetase
• length: 484
• theoretical pI: 4.97567
• theoretical MW: 56304.4
• GRAVY: -0.645041

⊟Function

• reaction:
  EC 6.1.1.17?  ExPASy
  Glutamate--tRNA ligaseATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu)
• TIGRFAM:
  Genetic information processingProtein synthesistRNA aminoacylationglutamate--tRNA ligase (TIGR00464; EC 6.1.1.17; HMM-score: 624.1)
  and 4 more
  Genetic information processingProtein synthesistRNA and rRNA base modificationglutamyl-queuosine tRNA(Asp) synthetase (TIGR03838; EC 6.1.1.-; HMM-score: 219.3)
  Genetic information processingProtein synthesistRNA aminoacylationglutamate--tRNA ligase (TIGR00463; EC 6.1.1.17; HMM-score: 127.8)
  Genetic information processingProtein synthesistRNA aminoacylationglutamine--tRNA ligase (TIGR00440; EC 6.1.1.18; HMM-score: 52.3)
  Genetic information processingProtein synthesistRNA aminoacylationlysine--tRNA ligase (TIGR00467; EC 6.1.1.6; HMM-score: 27.3)
• TheSEED:  
  Protein MetabolismProtein biosynthesistRNA aminoacylation, Glu and Gln Glutamyl-tRNA synthetase (EC 6.1.1.17)
  and 5 more
  Glutamyl-tRNA(Gln) synthetase (EC 6.1.1.24)
  tRNA glutamyl-Q(34)-Gln synthetase (EC 6.1.1.24)
  tRNA glutamyl-Q(34)-Glu synthetase (EC 6.1.1.17)
  tRNA synthetase relationships Glutamyl-tRNA synthetase (EC 6.1.1.17)
  Glutamyl-tRNA(Gln) synthetase (EC 6.1.1.24)
• PFAM:
  HUP (CL0039) tRNA-synt_1c; tRNA synthetases class I (E and Q), catalytic domain (PF00749; HMM-score: 414.3)
  and 2 more
  tRNA-synt_1f; tRNA synthetases class I (K) (PF01921; HMM-score: 18.7)
  no clan definedMrr_N; Mrr N-terminal domain (PF14338; HMM-score: 12.2)

⊟Structure, modifications & interactions

• domains:
• modifications:
• cofactors:
• effectors:
• protein partners:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 9.97
  • Cytoplasmic Membrane Score: 0
  • Cellwall Score: 0.01
  • Extracellular Score: 0.02
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular Possibility: 1
  • Signal Peptide Possibility: -1
  • N-terminally Anchored Score: -1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • Ymax: 0.133
  • Ymax_pos: 11
  • Cmax: 0.122
  • Cmax_pos: 35
  • Smax: 0.201
  • Smax_pos: 1
  • Smean: 0.174
  • D: 0.149
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 151220702 NCBI
• RefSeq: YP_001331524 NCBI
• UniProt: A6QEI0 UniProt

⊟Protein sequence

• MSDRIRVRYAPSPTGYLHIGNARTALFNYLYAKHYNGDFVIRIEDTDKKRNLEDGETSQFDNLKWLGLDWDESVDKDNGYGPYRQSERQHIYQPLIDQLLAEDKAYKCYMTEEELEAEREAQIARGEMPRYGGQHAHLTEEQRQQFEAEGRQPSIRFRVPQNQTYSFDDMVKGNISFDSNGIGDWVIVKKDGIPTYNFAVAIDDHYMQISDVIRGDDHISNTPKQIMIYEAFGWEPPRFGHMSLIVNEERKKLSKRDGQILQFIEQYRDLGYLPEALFNFIALLGWSPEGEEEIFSKEEFIKIFDEKRLSKSPAFFDKQKLAWVNNQYMKQKDTETVFQLALPHLIKANLIPEVPSEEDLSWGRKLIALYQKEMSYAGEIVPLSEMFFKEMPALGEEEQQVINGEQVPELMTHLFSKLEALEPFESAEIKKTIKEVQKETGIKGKQLFMPIRVAVTGQMHGPELPNTIEVLGKEKVLNRLKQYK

⊟Experimental data

• experimentally validated: no data available

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: gltX no polycistronic organisation predicted

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: data available for COL

⊟Protein stability

• half-life: no data available

⊟Other Information

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