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NCBI: 06-JUL-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_0544 [new locus tag: NWMN_RS03125 ]
  • pan locus tag?: SAUPAN002359000
  • symbol: ung
  • pan gene symbol?: ung
  • synonym:
  • product: uracil-DNA glycosylase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: NWMN_0544 [new locus tag: NWMN_RS03125 ]
  • symbol: ung
  • product: uracil-DNA glycosylase
  • replicon: chromosome
  • strand: +
  • coordinates: 628655..629311
  • length: 657
  • essential: unknown other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    ATGGAATGGTCGCAAATTTTTCATGACATAACAACGAAACATGACTTTAAAGCTATGCAT
    GATTTTTTAGAAAAAGAATATTCGACTGCAATCGTATACCCTGATAGGGAAAATATATAT
    CAAGCGTTTGATTTAACACCGTTTGAAAATATCAAAGTTGTTATATTAGGACAAGACCCG
    TATCATGGTCCAAACCAAGCACATGGATTAGCATTTTCAGTGCAACCTAACGCAAAATTC
    CCTCCATCTTTACGTAATATGTATAAAGAATTAGCAGATGATATTGGATGCGTTAGACAA
    ACACCGCATTTACAAGATTGGGCAAGAGAAGGCGTCTTGTTATTGAATACAGTTTTAACC
    GTAAGACAGGGTGAAGCAAATTCTCATCGTGATATTGGTTGGGAAACATTTACTGATGAA
    ATTATTAAAGCAGTGTCTGATTATAAAGAACATGTTGTCTTTATTTTGTGGGGGAAACCT
    GCACAGCAAAAAATAAAGCTTATCGATACATCTAAACATTGTATTATAAAATCAGTGCAT
    CCTAGTCCACTGTCTGCATATAGAGGATTCTTTGGATCAAAACCGTATTCCAAAGCGAAT
    GCCTATTTAGAGTCAGTAGGAAAATCACCAATTAATTGGTGTGAAAGTGAGGCGTAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    657

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: NWMN_0544 [new locus tag: NWMN_RS03125 ]
  • symbol: Ung
  • description: uracil-DNA glycosylase
  • length: 218
  • theoretical pI: 6.6759
  • theoretical MW: 24937.1
  • GRAVY: -0.425229

Function[edit source | edit]

  • reaction:
    EC 3.2.2.27?  ExPASy
    Uracil-DNA glycosylaseHydrolyzes single-stranded DNA or mismatched double-stranded DNA and polynucleotides, releasing free uracil
  • TIGRFAM:
    Genetic information processingDNA metabolismDNA replication, recombination, and repairuracil-DNA glycosylase (TIGR00628; EC 3.2.2.27; HMM-score: 263.2)
  • TheSEED:  
    DNA Repair Base Excision Uracil-DNA glycosylase, family 1 (EC 3.2.2.27) 
    Uracil-DNA glycosylase Uracil-DNA glycosylase, family 1 (EC 3.2.2.27) 
  • PFAM:
    no clan definedUDG; Uracil DNA glycosylase superfamily (PF03167; HMM-score: 92.4)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.105
    • Ymax_pos: 68
    • Cmax: 0.117
    • Cmax_pos: 19
    • Smax: 0.113
    • Smax_pos: 64
    • Smean: 0.066
    • D: 0.09
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MEWSQIFHDITTKHDFKAMHDFLEKEYSTAIVYPDRENIYQAFDLTPFENIKVVILGQDPYHGPNQAHGLAFSVQPNAKFPPSLRNMYKELADDIGCVRQTPHLQDWAREGVLLLNTVLTVRQGEANSHRDIGWETFTDEIIKAVSDYKEHVVFILWGKPAQQKIKLIDTSKHCIIKSVHPSPLSAYRGFFGSKPYSKANAYLESVGKSPINWCESEA

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]