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NCBI: 06-JUL-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_0553 [new locus tag: NWMN_RS03170 ]
  • pan locus tag?: SAUPAN002371000
  • symbol: mvaK1
  • pan gene symbol?: mvaK1
  • synonym:
  • product: mevalonate kinase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: NWMN_0553 [new locus tag: NWMN_RS03170 ]
  • symbol: mvaK1
  • product: mevalonate kinase
  • replicon: chromosome
  • strand: +
  • coordinates: 637050..637970
  • length: 921
  • essential: unknown other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    ATGACAAGAAAAGGATATGGGGAATCGACAGGTAAGATTATTTTAATAGGAGAACATGCT
    GTTACATTTGGAGAGCCTGCTATTGCAGTACCGTTTAACGCAGGTAAAATCAAAGTTTTA
    ATAGAAGCCTTAGAGAGCGGGAACTATTCGTCTATTAAAAGCGATGTTTACGATGGTATG
    TTATATGATGCGCCTGACCATCTTAAGTCTTTGGTGAACCGTTTTGTAGAATTAAATAAT
    ATTACAGAGCCGCTAGCAGTAACGATCCAAACGAATTTACCACCATCACGTGGATTAGGA
    TCGAGTGCAGCTGTCGCGGTTGCTTTTGTTCGTGCAAGTTATGATTTTTTAGGGAAATCA
    TTAACGAAAGAAGAACTCATTGAAAAGGCTAATTGGGCAGAGCAAATTGCACATGGTAAA
    CCAAGTGGTATTGATACGCAAACGATTGTATCAGGCAAACCAGTTTGGTTCCAAAAAGGT
    CATGCTGAAACGTTGAAAACGTTAAGTTTAGACGGCTATATGGTTGTTATAGATACTGGT
    GTGAAAGGTTCAACAAGACAAGCAGTAGAAGATGTTCATAAACTTTGTGAGGACCCTCAG
    TACATGTCACATGTAAAACATATCGGTAAGTTAGTTTTACGTGCGAGTGATGTGATTGAA
    CATCATAACTTTGAAGCCTTAGCGGATATTTTTAATGAATGTCATGCGGATTTAAAGGCG
    TTGACAGTTAGTCATGATAAAATAGAACAATTAATGAAAATTGGTAAAGAAAATGGTGCG
    ATTGCTGGAAAACTTACTGGCGCTGGTCGTGGTGGAAGTATGTTATTGCTTGCCAAAGAT
    TTACCAACAGCGAAAAATATTGTAAAAGCTGTAGAAAAAGCTGGTGCAGCACATACTTGG
    ATTGAGAATTTAGGAGGTTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    921

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: NWMN_0553 [new locus tag: NWMN_RS03170 ]
  • symbol: MvaK1
  • description: mevalonate kinase
  • length: 306
  • theoretical pI: 6.96804
  • theoretical MW: 32918.5
  • GRAVY: -0.0977124

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    MetabolismCentral intermediary metabolismOthermevalonate kinase (TIGR00549; EC 2.7.1.36; HMM-score: 290.8)
    MetabolismAmino acid biosynthesisAspartate familyhomoserine kinase (TIGR00191; EC 2.7.1.39; HMM-score: 46.8)
    MetabolismEnergy metabolismSugarsgalactokinase (TIGR00131; EC 2.7.1.6; HMM-score: 43.9)
    MetabolismAmino acid biosynthesisAromatic amino acid familyshikimate kinase (TIGR01920; EC 2.7.1.71; HMM-score: 36.2)
    MetabolismCentral intermediary metabolismOtherphosphomevalonate kinase (TIGR01220; EC 2.7.4.2; HMM-score: 25.8)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersOther4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase (TIGR00154; EC 2.7.1.148; HMM-score: 20.3)
    MetabolismCentral intermediary metabolismOtherdiphosphomevalonate decarboxylase (TIGR01240; EC 4.1.1.33; HMM-score: 14.3)
  • TheSEED:  
    Fatty Acids, Lipids, and IsoprenoidsIsoprenoidsArchaeal lipids Mevalonate kinase (EC 2.7.1.36) 
    IsoprenoidsIsoprenoid Biosynthesis Mevalonate kinase (EC 2.7.1.36) 
  • PFAM:
    S5 (CL0329) GHMP_kinases_N; GHMP kinases N terminal domain (PF00288; HMM-score: 55.9)
    no clan definedGHMP_kinases_C; GHMP kinases C terminal (PF08544; HMM-score: 45.4)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.219
    • Ymax_pos: 30
    • Cmax: 0.33
    • Cmax_pos: 30
    • Smax: 0.268
    • Smax_pos: 1
    • Smean: 0.124
    • D: 0.182
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MTRKGYGESTGKIILIGEHAVTFGEPAIAVPFNAGKIKVLIEALESGNYSSIKSDVYDGMLYDAPDHLKSLVNRFVELNNITEPLAVTIQTNLPPSRGLGSSAAVAVAFVRASYDFLGKSLTKEELIEKANWAEQIAHGKPSGIDTQTIVSGKPVWFQKGHAETLKTLSLDGYMVVIDTGVKGSTRQAVEDVHKLCEDPQYMSHVKHIGKLVLRASDVIEHHNFEALADIFNECHADLKALTVSHDKIEQLMKIGKENGAIAGKLTGAGRGGSMLLLAKDLPTAKNIVKAVEKAGAAHTWIENLGG

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor: SigB [1] other strains
    SigB (sigma factor) controls a large regulon involved in stress/starvation response and adaptation
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Markus Bischoff, Paul Dunman, Jan Kormanec, Daphne Macapagal, Ellen Murphy, William Mounts, Brigitte Berger-Bächi, Steven Projan
    Microarray-based analysis of the Staphylococcus aureus sigmaB regulon.
    J. Bacteriol.: 2004, 186(13);4085-99
    [PubMed:15205410] [WorldCat.org] [DOI] (P p)

Relevant publications[edit source | edit]