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NCBI: 06-JUL-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_0597 [new locus tag: NWMN_RS03405 ]
  • pan locus tag?: SAUPAN002498000
  • symbol: mnhE
  • pan gene symbol?: mnhE2
  • synonym:
  • product: putative monovalent cation/H+ antiporter subunit E

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: NWMN_0597 [new locus tag: NWMN_RS03405 ]
  • symbol: mnhE
  • product: putative monovalent cation/H+ antiporter subunit E
  • replicon: chromosome
  • strand: +
  • coordinates: 677136..677618
  • length: 483
  • essential: unknown other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    ATGAATCAAATAGTTTTAAATATTATCATTGCATTCTTATGGGTATTATTTCAAGATGAA
    GATCATTTTAAATTCTCGACTTTCTTTTCTGGATATCTAATTGGTTTAATTGTCATTTAT
    ATATTACACAGGTTTTTCAGCGATGATTTTTATGTTAGAAAAATATGGGTAGCTATTAAA
    TTTTTAGGTGTTTATTTATATCAATTAATAACATCTAGCATTAGCACGATTAATTATATT
    CTTTTTAAAACAAAAGATATGAACCCTGGATTACTTTCATATGAAACAAGACTAACAAGT
    GATTGGTCAATAACATTTTTAACAATTTTAATTATTATAACTCCAGGGTCTACAGTAATA
    CGAATTTCTCAAGACTCTAAAAAGTTTTTTATTCATAGTATCGACGTGTCAGAAAAAGAA
    AAAGATAGTTTGTTAAGAAGTATTAAGCATTATGAAGACTTAATATTGGAGGTGTCGCGA
    TGA
    60
    120
    180
    240
    300
    360
    420
    480
    483

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: NWMN_0597 [new locus tag: NWMN_RS03405 ]
  • symbol: MnhE
  • description: putative monovalent cation/H+ antiporter subunit E
  • length: 160
  • theoretical pI: 7.80816
  • theoretical MW: 18850.9
  • GRAVY: 0.42125

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    MetabolismTransport and binding proteinsCations and iron carrying compoundsmulticomponent Na+:H+ antiporter (TIGR00942; HMM-score: 79.2)
  • TheSEED:  
    Membrane TransportUni- Sym- and AntiportersSodium Hydrogen Antiporter Na(+) H(+) antiporter subunit E (TC 2.A.63.1.3) 
  • PFAM:
    no clan definedMNHE; Na+/H+ ion antiporter subunit (PF01899; HMM-score: 110.8)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 3
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular Possibility: 0
    • Signal Peptide Possibility: -0.5
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.102
    • Ymax_pos: 24
    • Cmax: 0.17
    • Cmax_pos: 24
    • Smax: 0.114
    • Smax_pos: 1
    • Smean: 0.068
    • D: 0.089
  • predicted transmembrane helices (TMHMM): 3

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MNQIVLNIIIAFLWVLFQDEDHFKFSTFFSGYLIGLIVIYILHRFFSDDFYVRKIWVAIKFLGVYLYQLITSSISTINYILFKTKDMNPGLLSYETRLTSDWSITFLTILIIITPGSTVIRISQDSKKFFIHSIDVSEKEKDSLLRSIKHYEDLILEVSR

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor: SigB [1] [2] other strains
    SigB (sigma factor) controls a large regulon involved in stress/starvation response and adaptation
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Bettina Schulthess, Dominik A Bloes, Patrice François, Myriam Girard, Jacques Schrenzel, Markus Bischoff, Brigitte Berger-Bächi
    The σB-dependent yabJ-spoVG operon is involved in the regulation of extracellular nuclease, lipase, and protease expression in Staphylococcus aureus.
    J. Bacteriol.: 2011, 193(18);4954-62
    [PubMed:21725011] [WorldCat.org] [DOI] (I p)
  2. Markus Bischoff, Paul Dunman, Jan Kormanec, Daphne Macapagal, Ellen Murphy, William Mounts, Brigitte Berger-Bächi, Steven Projan
    Microarray-based analysis of the Staphylococcus aureus sigmaB regulon.
    J. Bacteriol.: 2004, 186(13);4085-99
    [PubMed:15205410] [WorldCat.org] [DOI] (P p)

Relevant publications[edit source | edit]