From AureoWiki
Jump to: navigation, search
NCBI: 06-JUL-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_0629 [new locus tag: NWMN_RS03575 ]
  • pan locus tag?: SAUPAN002539000
  • symbol: NWMN_0629
  • pan gene symbol?: graS
  • synonym:
  • product: hypothetical protein

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: NWMN_0629 [new locus tag: NWMN_RS03575 ]
  • symbol: NWMN_0629
  • product: hypothetical protein
  • replicon: chromosome
  • strand: +
  • coordinates: 710043..711083
  • length: 1041
  • essential: unknown other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    ATGAATAATTTGAAATGGGTAGCTTATTTTTTGAAATCTCGCATGAACTGGATATTTTGG
    ATATTGTTTTTAAACTTCCTTATGTTAGGCATTAGTCTAATCGATTATGATTTTCCAATA
    GACAGTTTATTTTATATTGTTTCTTTGAATTTAAGTTTAACAATGATTTTTCTTTTATTG
    ACATATTTTAAAGAAGTAAAATTATATAAGCATTTTGACAAAGATAAAGAAATAGAAGAA
    ATTAAACATAAAGATTTAGCGGAAACGCCATTTCAACGTCATACAGTTGATTATTTATAT
    CGTCAAATCTCAGCGCACAAAGAAAAGGTTGTTGAGCAACAGTTGCAATTGAACATGCAT
    GAACAAACCATTACAGAATTTGTGCACGACATAAAAACACCTGTGACAGCTATGAAATTA
    TTAATTGATCAAGAAAAAAATCAAGAAAGAAAACAAGCATTACTATATGAATGGTCTCGT
    ATAAACTCGATGCTAGATACACAGCTGTATATTACTAGATTAGAATCTCAACGTAAAGAT
    ATGTATTTTGATTACGTGTCACTTAAACGCATGGTCATTGATGAAATACAATTAACAAGA
    CATATTAGTCAGGTTAAAGGTATTGGTTTTGATGTTGACTTTAAAGTGGATGATTATGTT
    TATACAGATATAAAATGGTGTCGTATGATTATTAGACAAATTTTGTCAAACGCATTGAAA
    TATAGTGAGAATTTTAATATTGAAATTGGGACAGAATTAAATGATCAACATGTTTCGTTA
    TATATTAAAGACTATGGCAGAGGTATTAGTAAAAAAGATATGCCGCGAATATTTGAACGA
    GGATTTACGTCAACGGCTAACAGAAATGAAACGACGTCTTCAGGTATGGGTCTATATTTA
    GTAAATAGTGTAAAGGATCAATTAGGTATTCACCTGCAAGTCACGTCGACTGTTGGTAAG
    GGGACAACTGTCAGATTGATTTTCCCATTACAAAATGAAATTGTTGAACGCATGTCGGAA
    GTGACAAATTTGTCATTTTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1041

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: NWMN_0629 [new locus tag: NWMN_RS03575 ]
  • symbol: NWMN_0629
  • description: hypothetical protein
  • length: 346
  • theoretical pI: 7.34071
  • theoretical MW: 41078.2
  • GRAVY: -0.238439

Function[edit source | edit]

  • reaction:
    EC 2.7.13.3?  ExPASy
    Histidine kinaseATP + protein L-histidine = ADP + protein N-phospho-L-histidine
  • TIGRFAM:
    Signal transductionSignal transductionTwo-component systemsphosphate regulon sensor kinase PhoR (TIGR02966; EC 2.7.3.-; HMM-score: 89.1)
    heavy metal sensor kinase (TIGR01386; EC 2.7.13.3; HMM-score: 66)
    Signal transductionSignal transductionTwo-component systemsTMAO reductase sytem sensor TorS (TIGR02956; EC 2.7.13.3; HMM-score: 60.5)
    Genetic information processingProtein fateProtein and peptide secretion and traffickingputative PEP-CTERM system histidine kinase (TIGR02916; EC 2.7.13.3; HMM-score: 40.1)
    Signal transductionSignal transductionTwo-component systemsputative PEP-CTERM system histidine kinase (TIGR02916; EC 2.7.13.3; HMM-score: 40.1)
    MetabolismCentral intermediary metabolismNitrogen fixationnitrogen fixation negative regulator NifL (TIGR02938; HMM-score: 25.3)
    Signal transductionRegulatory functionsProtein interactionsnitrogen fixation negative regulator NifL (TIGR02938; HMM-score: 25.3)
    Cellular processesCellular processesSporulation and germinationanti-sigma F factor (TIGR01925; EC 2.7.11.1; HMM-score: 23.2)
    Signal transductionRegulatory functionsProtein interactionsanti-sigma F factor (TIGR01925; EC 2.7.11.1; HMM-score: 23.2)
    Genetic information processingDNA metabolismDNA replication, recombination, and repairDNA mismatch repair protein MutL (TIGR00585; HMM-score: 14.6)
    Genetic information processingDNA metabolismDNA replication, recombination, and repairDNA topoisomerase VI, B subunit (TIGR01052; EC 5.99.1.3; HMM-score: 13.4)
  • TheSEED:  
    Virulence, Disease and DefenseBacteriocins, ribosomally synthesized antibacterial peptidesBacitracin Stress Response Two-component sensor histidine kinase BceS 
  • PFAM:
    His_Kinase_A (CL0025) HATPase_c; Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase (PF02518; HMM-score: 69.2)
    HATPase_c_3; Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase (PF13589; HMM-score: 17.4)
    HATPase_c_2; Histidine kinase-like ATPase domain (PF13581; HMM-score: 17.4)
    no clan definedTA0956; Thermoplasma acidophilum protein TA0956 (PF11513; HMM-score: 14.5)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 1.05
    • Cytoplasmic Membrane Score: 8.78
    • Cellwall Score: 0.08
    • Extracellular Score: 0.09
    • Internal Helices: 2
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular Possibility: 0.17
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.122
    • Ymax_pos: 11
    • Cmax: 0.119
    • Cmax_pos: 35
    • Smax: 0.217
    • Smax_pos: 3
    • Smean: 0.142
    • D: 0.13
  • predicted transmembrane helices (TMHMM): 2

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MNNLKWVAYFLKSRMNWIFWILFLNFLMLGISLIDYDFPIDSLFYIVSLNLSLTMIFLLLTYFKEVKLYKHFDKDKEIEEIKHKDLAETPFQRHTVDYLYRQISAHKEKVVEQQLQLNMHEQTITEFVHDIKTPVTAMKLLIDQEKNQERKQALLYEWSRINSMLDTQLYITRLESQRKDMYFDYVSLKRMVIDEIQLTRHISQVKGIGFDVDFKVDDYVYTDIKWCRMIIRQILSNALKYSENFNIEIGTELNDQHVSLYIKDYGRGISKKDMPRIFERGFTSTANRNETTSSGMGLYLVNSVKDQLGIHLQVTSTVGKGTTVRLIFPLQNEIVERMSEVTNLSF

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]