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NCBI: 06-JUL-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_0656 [new locus tag: NWMN_RS03720 ]
  • pan locus tag?: SAUPAN002571000
  • symbol: NWMN_0656
  • pan gene symbol?:
  • synonym:
  • product: cobalamin synthesis protein, P47K family protein

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: NWMN_0656 [new locus tag: NWMN_RS03720 ]
  • symbol: NWMN_0656
  • product: cobalamin synthesis protein, P47K family protein
  • replicon: chromosome
  • strand: +
  • coordinates: 737427..738353
  • length: 927
  • essential: unknown other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    ATGAAAAATAATAAAGATGAAAAAATAAGAATATCCATAATTAACGGATTTTTGGGTAGT
    GGTAAAACCACGTTACTGACACATTATATTAGTGAATTATTAAAAAATGATGAGAAAATT
    AAAATCATCATGAATGAATTCGGTACTTTTGATATTGATAGCAATAGTATTTCAAATGAA
    ATTGAAGTCCATTCATTGATTAATGGTTGTGTTTGTTGCGATCTTAAACAAGAACTTGTC
    TATGAACTAAAAGCCATTGCTTTAAAAGGGGACGTTAATCATGTCATCATAGAAGCGACA
    GGCATTGCGCATCCTTTGGAATTACTAGTTGCATGTCAAGATCCGCAAATCGTTAATTTC
    TTTGAAAAGCCGATTATTTATGGTGTATTAGATGCGACTCGATTTTTAGAACGTCATCAA
    TATACCGAAAATACAGTTTCGCTGATGGAAGATCAGTTGAAACTAAGTGACATGATTATT
    ATTAATAAAATTGATCTTATAACTGATGACAGTCTTGAGAAAATTGATAAGCAATTAGGT
    ATGATTTGTGCAAGTATTCCAACTTATAAAACAACCTATGGAAAAGTTTCGTTGGAAGAA
    TTGGACTTAACTGTTAAAGACAGAGAGATATCGTCTCATCATCACCATCATCATGGGATT
    AAAAGTATGACTTACACGTTTACAGGTCCGATTGATCGTCATTTGTTTTATCAATTTATA
    ATGAAATTACCGGAATCTGTTCTACGTTTGAAAGGTTATGTGTCATTTAGAGATCAACCA
    AATGCAATTTATGAATTTCAATATGCATATGGTTTACCAGACTATGGAATAATTGGCATG
    CAATTACCATTAACGATTGTTATTATTGGTGAAACTTTAGATACAAATCACATACGTAAT
    CAATTGGATATGCTACAATTTACGTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    927

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: NWMN_0656 [new locus tag: NWMN_RS03720 ]
  • symbol: NWMN_0656
  • description: cobalamin synthesis protein, P47K family protein
  • length: 308
  • theoretical pI: 5.52628
  • theoretical MW: 35224.4
  • GRAVY: -0.0918831

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersHeme, porphyrin, and cobalamincobalamin biosynthesis protein CobW (TIGR02475; HMM-score: 101.4)
    Genetic information processingProtein fateProtein modification and repairhydrogenase accessory protein HypB (TIGR00073; HMM-score: 22.6)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersMolybdopterinmolybdopterin-guanine dinucleotide biosynthesis protein B (TIGR00176; HMM-score: 15.6)
    KaiC domain protein, AF_0351 family (TIGR03880; HMM-score: 13.6)
  • TheSEED:  
    Putative metal chaperone, involved in Zn homeostasis, GTPase of COG0523 family 
  • PFAM:
    P-loop_NTPase (CL0023) cobW; CobW/HypB/UreG, nucleotide-binding domain (PF02492; HMM-score: 152.1)
    no clan definedCobW_C; Cobalamin synthesis protein cobW C-terminal domain (PF07683; HMM-score: 52.1)
    P-loop_NTPase (CL0023) MobB; Molybdopterin guanine dinucleotide synthesis protein B (PF03205; HMM-score: 20.5)
    RsgA_GTPase; RsgA GTPase (PF03193; HMM-score: 16.7)
    ATPase_2; ATPase domain predominantly from Archaea (PF01637; HMM-score: 16.4)
    AAA_14; AAA domain (PF13173; HMM-score: 16.3)
    no clan definedDUF2824; Protein of unknown function (DUF2824) (PF11039; HMM-score: 14.9)
    P-loop_NTPase (CL0023) AAA_22; AAA domain (PF13401; HMM-score: 14.3)
    NACHT; NACHT domain (PF05729; HMM-score: 13.5)
    T2SSE; Type II/IV secretion system protein (PF00437; HMM-score: 12)
    Arf; ADP-ribosylation factor family (PF00025; HMM-score: 12)
    AAA_30; AAA domain (PF13604; HMM-score: 11.1)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.169
    • Ymax_pos: 11
    • Cmax: 0.11
    • Cmax_pos: 37
    • Smax: 0.373
    • Smax_pos: 1
    • Smean: 0.268
    • D: 0.208
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MKNNKDEKIRISIINGFLGSGKTTLLTHYISELLKNDEKIKIIMNEFGTFDIDSNSISNEIEVHSLINGCVCCDLKQELVYELKAIALKGDVNHVIIEATGIAHPLELLVACQDPQIVNFFEKPIIYGVLDATRFLERHQYTENTVSLMEDQLKLSDMIIINKIDLITDDSLEKIDKQLGMICASIPTYKTTYGKVSLEELDLTVKDREISSHHHHHHGIKSMTYTFTGPIDRHLFYQFIMKLPESVLRLKGYVSFRDQPNAIYEFQYAYGLPDYGIIGMQLPLTIVIIGETLDTNHIRNQLDMLQFT

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]