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NCBI: 06-JUL-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_0740 [new locus tag: NWMN_RS04190 ]
  • pan locus tag?: SAUPAN002705000
  • symbol: gapR
  • pan gene symbol?: gapR
  • synonym:
  • product: glycolytic operon regulator

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: NWMN_0740 [new locus tag: NWMN_RS04190 ]
  • symbol: gapR
  • product: glycolytic operon regulator
  • replicon: chromosome
  • strand: +
  • coordinates: 832890..833903
  • length: 1014
  • essential: unknown other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    GTGAAAGACTTATTGCAAGCACAGCAAAAGCTTATACCGGATCTCATAGATAAAATGTAT
    AAACGTTTTTCTATTCTTACTACTATCTCAAAAAATCAGCCTGTCGGACGTCGAAGTTTA
    AGCGAACATATGGATATGACTGAACGTGTACTGCGTTCTGAAACAGATATGCTTAAGAAA
    CAAGATTTGATAAAAGTTAAGCCTACCGGAATGGAAATTACAGCTGAAGGTGAGCAACTG
    ATTTCGCAATTGAAAGGTTACTTTGATATCTATGCAGATGATAATCGTCTGTCAGAAGGT
    ATTAAGAATAAATTTCAAATTAAGGAAGTTCATGTTGTTCCTGGTGATGCTGATAATAGT
    CAATCTGTTAAAACAGAATTAGGTAGACAAGCAGGTCAATTACTTGAAGGCATATTACAA
    GAAGACGCGATAGTTGCTGTAACTGGCGGATCCACGATGGCATGTGTTAGTGAAGCAATT
    CATTTATTACCATATAATGTATTCTTCGTACCAGCCAGAGGTGGACTAGGCGAAAATGTT
    GTCTTTCAGGCAAACACAATTGCAGCCAGTATGGCACAACAAGCTGGCGGTTATTATACG
    ACGATGTATGTACCTGATAATGTCAGTGAAACAACATATAATACATTGTTGTTAGAGCCA
    TCAGTCATAAACACTTTAGACAAAATTAAACAAGCAAACGTTATATTACACGGCATTGGT
    GATGCGCTGAAGATGGCGCATCGACGTCAATCACCTGAAAAGGTCATTGAACAACTTCAA
    CATCATCAAGCTGTCGGAGAGGCATTTGGTTATTATTTTGATACACAAGGTCAAATTGTC
    CATAAGGTTAAAACAATTGGACTTCAATTAGAAGACCTTGAATCAAAAGACTTTATTTTT
    GCAGTTGCAGGAGGCAAATCGAAAGGTGAAGCAATTAAAGCATACTTGACGATTGCACCC
    AAGAATACAGTGTTAATCACTGATGAAGCCGCAGCAAAGATAATACTTGAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1014

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: NWMN_0740 [new locus tag: NWMN_RS04190 ]
  • symbol: GapR
  • description: glycolytic operon regulator
  • length: 337
  • theoretical pI: 6.11378
  • theoretical MW: 37210.4
  • GRAVY: -0.196736

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    Genetic information processingDNA metabolismDNA replication, recombination, and repairDnaD family protein (TIGR04548; HMM-score: 16.8)
  • TheSEED:  
    Central glycolytic genes regulator 
  • PFAM:
    ISOCOT_Fold (CL0246) Sugar-bind; Putative sugar-binding domain (PF04198; HMM-score: 248.2)
    HTH (CL0123) MotA_activ; Transcription factor MotA, activation domain (PF09114; HMM-score: 15.9)
    HTH_45; Winged helix-turn-helix (PF14947; HMM-score: 15.5)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.131
    • Ymax_pos: 41
    • Cmax: 0.133
    • Cmax_pos: 41
    • Smax: 0.206
    • Smax_pos: 37
    • Smean: 0.104
    • D: 0.12
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MKDLLQAQQKLIPDLIDKMYKRFSILTTISKNQPVGRRSLSEHMDMTERVLRSETDMLKKQDLIKVKPTGMEITAEGEQLISQLKGYFDIYADDNRLSEGIKNKFQIKEVHVVPGDADNSQSVKTELGRQAGQLLEGILQEDAIVAVTGGSTMACVSEAIHLLPYNVFFVPARGGLGENVVFQANTIAASMAQQAGGYYTTMYVPDNVSETTYNTLLLEPSVINTLDKIKQANVILHGIGDALKMAHRRQSPEKVIEQLQHHQAVGEAFGYYFDTQGQIVHKVKTIGLQLEDLESKDFIFAVAGGKSKGEAIKAYLTIAPKNTVLITDEAAAKIILE

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor: SigB [1] other strains
    SigB (sigma factor) controls a large regulon involved in stress/starvation response and adaptation
  • regulator: GapR
    GapR (TF) important in Glycolysis  RegPrecise

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Bettina Schulthess, Dominik A Bloes, Patrice François, Myriam Girard, Jacques Schrenzel, Markus Bischoff, Brigitte Berger-Bächi
    The σB-dependent yabJ-spoVG operon is involved in the regulation of extracellular nuclease, lipase, and protease expression in Staphylococcus aureus.
    J. Bacteriol.: 2011, 193(18);4954-62
    [PubMed:21725011] [WorldCat.org] [DOI] (I p)

Relevant publications[edit source | edit]