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NCBI: 06-JUL-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_0756 [new locus tag: NWMN_RS04280 ]
  • pan locus tag?: SAUPAN002779000
  • symbol: clfA
  • pan gene symbol?: clfA
  • synonym:
  • product: clumping factor A

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: NWMN_0756 [new locus tag: NWMN_RS04280 ]
  • symbol: clfA
  • product: clumping factor A
  • replicon: chromosome
  • strand: +
  • coordinates: 849757..852558
  • length: 2802
  • essential: unknown other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGAATATGAAGAAAAAAGAAAAACACGCAATTCGGAAAAAATCGATTGGCGTGGCTTCA
    GTGCTTGTAGGTACGTTAATCGGTTTTGGACTACTCAGCAGTAAAGAAGCAGATGCAAGT
    GAAAATAGTGTTACGCAATCTGATAGCGCAAGTAACGAAAGCAAAAGTAATGATTCAAGT
    AGCGTTAGTGCTGCACCTAAAACAGACGACACAAACGTGAGTGATACTAAAACATCGTCA
    AACACTAATAATGGCGAAACGAGTGTGGCGCAAAATCCAGCACAACAGGAAACGACACAA
    TCATCATCAACAAATGCAACTACGGAAGAAACGCCGGTAACTGGTGAAGCTACTACTACG
    ACAACGAATCAAGCTAATACACCGGCAACAACTCAATCAAGCAATACAAATGCGGAGGAA
    TTAGTGAATCAAACAAGTAATGAAACGACTTTTAATGATACTAATACAGTATCATCTGTA
    AATTCACCTCAAAATTCTACAAATGCGGAAAATGTTTCAACAACGCAAGATACTTCAACT
    GAAGCAACACCTTCAAACAATGAATCAGCTCCACAGAGTACAGATGCAAGTAATAAAGAT
    GTAGTTAATCAAGCGGTTAATACAAGTGCGCCTAGAATGAGAGCATTTAGTTTAGCGGCA
    GTAGCTGCAGATGCACCGGCAGCTGGCACAGATATTACGAATCAGTTGACGAATGTGACA
    GTTGGTATTGACTCTGGTACGACTGTGTATCCGCACCAAGCAGGTTATGTCAAACTGAAT
    TATGGTTTTTCAGTGCCTAATTCTGCTGTTAAAGGTGACACATTCAAAATAACTGTACCT
    AAAGAATTAAACTTAAATGGTGTAACTTCAACTGCTAAAGTGCCACCAATTATGGCTGGA
    GATCAAGTATTGGCAAATGGTGTAATCGATAGTGATGGTAATGTTATTTATACATTTACA
    GACTATGTAAATACTAAAGATGATGTAAAAGCAACTTTGACCATGCCCGCTTATATTGAC
    CCTGAAAATGTTAAAAAGACAGGTAATGTGACATTGGCTACTGGCATAGGTAGTACAACA
    GCAAACAAAACAGTATTAGTAGATTATGAAAAATATGGTAAGTTTTATAACTTATCTATT
    AAAGGTACAATTGACCAAATCGATAAAACAAATAATACGTATCGTCAGACAATTTATGTC
    AATCCAAGTGGAGATAACGTTATTGCGCCGGTTTTAACAGGTAATTTAAAACCAAATACG
    GATAGTAATGCATTAATAGATCAGCAAAATACAAGTATTAAAGTATATAAAGTAGATAAT
    GCAGCTGATTTATCTGAAAGTTACTTTGTGAATCCAGAAAACTTTGAGGATGTCACTAAT
    AGTGTGAATATTACATTCCCAAATCCAAATCAATATAAAGTAGAGTTTAATACGCCTGAT
    GATCAAATTACAACACCGTATATAGTAGTTGTTAATGGTCATATTGATCCGAATAGCAAA
    GGTGATTTAGCTTTACGTTCAACTTTATATGGGTATAACTCGAATATAATTTGGCGCTCT
    ATGTCATGGGACAACGAAGTAGCATTTAATAACGGATCAGGTTCTGGTGACGGTATCGAT
    AAACCAGTTGTTCCTGAACAACCTGATGAGCCTGGTGAAATTGAACCAATTCCAGAGGAT
    TCAGATTCTGACCCAGGTTCAGATTCTGGCAGCGATTCTAATTCAGATAGCGGTTCAGAT
    TCGGGTAGTGATTCTACATCAGATAGTGGTTCAGATTCAGCGAGTGATTCAGATTCAGCA
    AGTGATTCAGACTCAGCGAGTGATTCAGATTCAGCAAGCGATTCCGACTCAGCGAGCGAT
    TCCGACTCAGACAATGACTCGGATTCAGATAGCGATTCTGACTCAGACAGTGACTCAGAT
    TCCGACAGTGACTCAGATTCAGATAGCGATTCTGACTCAGACAGTGACTCAGATTCAGAT
    AGCGATTCAGATTCAGATAGCGATTCAGATTCCGACAGTGATTCCGACTCAGACAGCGAT
    TCTGACTCCGACAGTGATTCCGACTCAGACAGCGATTCAGATTCCGACAGTGATTCCGAC
    TCAGATAGCGATTCCGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGATTCAGAC
    AGCGATTCAGATTCAGATAGCGATTCAGATTCCGACAGTGACTCAGATTCCGACAGTGAC
    TCGGATTCAGATAGCGATTCAGATTCCGACAGTGACTCAGATTCCGACAGTGACTCAGAC
    TCAGACAGTGATTCGGATTCAGCGAGTGATTCGGATTCAGATAGTGATTCCGACTCCGAC
    AGTGACTCGGATTCAGATAGCGACTCAGACTCGGATAGCGACTCGGATTCAGATAGCGAT
    TCGGACTCAGATAGCGATTCAGAATCAGACAGCGATTCAGAATCAGACAGCGATTCAGAT
    TCAGACAGCGACTCAGACAGTGACTCAGATTCAGATAGTGACTCGGATTCAGCGAGTGAT
    TCAGACTCAGGTAGTGACTCCGATTCATCAAGTGATTCCGACTCAGAAAGTGATTCAAAT
    AGCGATTCCGAGTCAGGTTCTAACAATAATGTAGTTCCGCCTAATTCACCTAAAAATGGT
    ACTAATGCTTCTAATAAAAATGAGGCTAAAGATAGTAAAGAACCATTACCAGATACAGGT
    TCTGAAGATGAAGCAAATACGTCACTAATTTGGGGATTATTAGCATCAATAGGTTCATTA
    CTACTTTTCAGAAGAAAAAAAGAAAATAAAGATAAGAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1740
    1800
    1860
    1920
    1980
    2040
    2100
    2160
    2220
    2280
    2340
    2400
    2460
    2520
    2580
    2640
    2700
    2760
    2802

Protein[edit source | edit]

Protein Data Bank: 1N67

General[edit source | edit]

  • locus tag: NWMN_0756 [new locus tag: NWMN_RS04280 ]
  • symbol: ClfA
  • description: clumping factor A
  • length: 933
  • theoretical pI: 3.44162
  • theoretical MW: 97057.3
  • GRAVY: -1.08264

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    gram-positive signal peptide, YSIRK family (TIGR01168; HMM-score: 28.4)
    Cell structureCell envelopeOtherLPXTG cell wall anchor domain (TIGR01167; HMM-score: 27.7)
  • TheSEED:  
    Virulence, Disease and DefenseAdhesionAdhesins in Staphylococcus Clumping factor ClfA, fibrinogen-binding protein 
  • PFAM:
    Adhesin (CL0204) SdrG_C_C; C-terminus of bacterial fibrinogen-binding adhesin (PF10425; HMM-score: 149.1)
    no clan definedYSIRK_signal; YSIRK type signal peptide (PF04650; HMM-score: 34.3)
    Gram_pos_anchor; LPXTG cell wall anchor motif (PF00746; HMM-score: 25.6)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cellwall
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 10
    • Extracellular Score: 0
    • Internal Helices: 0
  • LocateP: LPxTG Cell-wall anchored
    • Prediction by SwissProt Classification: Cell Wall
    • Pathway Prediction: Sec-(SPI)
    • Intracellular Possibility: 0
    • Signal Peptide Possibility: 1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: found LPxTG motif :LPDTG
  • SignalP: Signal peptide length 40 aa
    • Ymax: 0.669
    • Ymax_pos: 40
    • Cmax: 0.557
    • Cmax_pos: 40
    • Smax: 0.893
    • Smax_pos: 6
    • Smean: 0.79
    • D: 0.716
  • predicted transmembrane helices (TMHMM): 1

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MNMKKKEKHAIRKKSIGVASVLVGTLIGFGLLSSKEADASENSVTQSDSASNESKSNDSSSVSAAPKTDDTNVSDTKTSSNTNNGETSVAQNPAQQETTQSSSTNATTEETPVTGEATTTTTNQANTPATTQSSNTNAEELVNQTSNETTFNDTNTVSSVNSPQNSTNAENVSTTQDTSTEATPSNNESAPQSTDASNKDVVNQAVNTSAPRMRAFSLAAVAADAPAAGTDITNQLTNVTVGIDSGTTVYPHQAGYVKLNYGFSVPNSAVKGDTFKITVPKELNLNGVTSTAKVPPIMAGDQVLANGVIDSDGNVIYTFTDYVNTKDDVKATLTMPAYIDPENVKKTGNVTLATGIGSTTANKTVLVDYEKYGKFYNLSIKGTIDQIDKTNNTYRQTIYVNPSGDNVIAPVLTGNLKPNTDSNALIDQQNTSIKVYKVDNAADLSESYFVNPENFEDVTNSVNITFPNPNQYKVEFNTPDDQITTPYIVVVNGHIDPNSKGDLALRSTLYGYNSNIIWRSMSWDNEVAFNNGSGSGDGIDKPVVPEQPDEPGEIEPIPEDSDSDPGSDSGSDSNSDSGSDSGSDSTSDSGSDSASDSDSASDSDSASDSDSASDSDSASDSDSDNDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSASDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSESDSDSESDSDSDSDSDSDSDSDSDSDSDSASDSDSGSDSDSSSDSDSESDSNSDSESGSNNNVVPPNSPKNGTNASNKNEAKDSKEPLPDTGSEDEANTSLIWGLLASIGSLLLFRRKKENKDKK

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor: SigB [1] [2] other strains
    SigB (sigma factor) controls a large regulon involved in stress/starvation response and adaptation
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Bettina Schulthess, Dominik A Bloes, Patrice François, Myriam Girard, Jacques Schrenzel, Markus Bischoff, Brigitte Berger-Bächi
    The σB-dependent yabJ-spoVG operon is involved in the regulation of extracellular nuclease, lipase, and protease expression in Staphylococcus aureus.
    J. Bacteriol.: 2011, 193(18);4954-62
    [PubMed:21725011] [WorldCat.org] [DOI] (I p)
  2. Markus Bischoff, Paul Dunman, Jan Kormanec, Daphne Macapagal, Ellen Murphy, William Mounts, Brigitte Berger-Bächi, Steven Projan
    Microarray-based analysis of the Staphylococcus aureus sigmaB regulon.
    J. Bacteriol.: 2004, 186(13);4085-99
    [PubMed:15205410] [WorldCat.org] [DOI] (P p)

Relevant publications[edit source | edit]