From AureoWiki
Jump to: navigation, search
NCBI: 06-JUL-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_0833 [new locus tag: NWMN_RS04700 ]
  • pan locus tag?: SAUPAN003070000
  • symbol: pgi
  • pan gene symbol?: pgi
  • synonym:
  • product: glucose-6-phosphate isomerase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: NWMN_0833 [new locus tag: NWMN_RS04700 ]
  • symbol: pgi
  • product: glucose-6-phosphate isomerase
  • replicon: chromosome
  • strand: +
  • coordinates: 921644..922975
  • length: 1332
  • essential: unknown other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    ATGACTCATATTCAATTAGATTTTAGTAAAACGTTAGAATTTTTCGGTGAACACGAATTA
    AAACAACAACAAGAAATTGTTAAATCAATTCACAAAACAATTCATGAAGGTACTGGTGCA
    GGTAGTGACTTCTTAGGCTGGGTTGATTTACCAGTTGATTACGACAAAGAAGAATTTTCA
    AGAATTGTTGAAGCATCAAAACGCATTAAAGAAAATTCTGATGTTTTAGTAGTCATCGGT
    ATTGGTGGTTCTTACTTAGGTGCACGTGCAGCAATCGAAATGTTAACGTCATCATTTAGA
    AACAGCAATGAATACCCTGAAATTGTATTTGTTGGTAATCACTTATCATCAACATATACG
    AAAGAGTTAGTTGATTATTTAGCAGACAAAGATTTCTCTGTAAACGTTATTTCTAAATCT
    GGTACAACTACAGAACCAGCAGTTGCATTTAGATTGTTCAAACAATTAGTTGAAGAAAGA
    TACGGTAAAGAAGAAGCACAAAAACGTATATTTGCAACAACGGATAAAGAAAAAGGTGCT
    TTAAAACAGTTGGCTACAAACGAAGGTTATGAAACGTTTATCGTACCTGATGATGTAGGT
    GGAAGATATTCTGTTTTAACAGCAGTAGGATTATTACCAATTGCAACAGCTGGAATTAAC
    ATCGAAGCTATGATGATTGGTGCTGCAAAAGCACGTGAAGAATTATCTTCAGATAAATTA
    GAAGAAAACATTGCATACCAATATGCGACAATTCGAAACATTTTATATGCAAAAGGTTAT
    ACAACAGAAATGTTGATTAACTATGAACCATCTATGCAATACTTTAATGAATGGTGGAAA
    CAATTATTTGGTGAATCAGAAGGTAAAGACTTCAAAGGTATCTATCCTTCAAGTGCCAAC
    TACACAACTGATTTACATTCTTTAGGTCAATATGTACAAGAAGGCCGTCGCTTCTTATTC
    GAAACAGTTGTAAAAGTAAATCATCCTAAATATGATATTACTATTGAAAAAGATAGTGAT
    GATCTAGACGGATTAAATTATTTAGCTGGTAAAACAATCGACGAAGTTAACACAAAAGCA
    TTCGAAGGTACATTATTAGCGCATACTGATGGTGGTGTTCCTAACATGGTAGTGAACATT
    CCACAATTAGATGAAGAAACTTTCGGTTACGTCGTATACTTCTTCGAACTTGCTTGTGCA
    ATGAGTGGATACCAATTAGGCGTAAATCCATTTAACCAACCTGGTGTAGAAGCATATAAA
    CAAAACATGTTCGCATTATTAGGTAAACCTGGTTTTGAAGACTTGAAAAAAGAATTAGAA
    GAACGTTTATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1332

Protein[edit source | edit]

Protein Data Bank: 3FF1

General[edit source | edit]

  • locus tag: NWMN_0833 [new locus tag: NWMN_RS04700 ]
  • symbol: Pgi
  • description: glucose-6-phosphate isomerase
  • length: 443
  • theoretical pI: 4.54735
  • theoretical MW: 49807.8
  • GRAVY: -0.325959

Function[edit source | edit]

  • reaction:
    EC 5.3.1.9?  ExPASy
    Glucose-6-phosphate isomeraseD-glucose 6-phosphate = D-fructose 6-phosphate
  • TIGRFAM:
    bifunctional phosphoglucose/phosphomannose isomerase (TIGR02128; EC 5.3.1.9; HMM-score: 25.6)
    dihydroxyacetone kinase, phosphotransfer subunit (TIGR02364; HMM-score: 13.8)
  • TheSEED:  
    CarbohydratesCentral carbohydrate metabolismGlycolysis and Gluconeogenesis Glucose-6-phosphate isomerase (EC 5.3.1.9) 
    One-carbon MetabolismFormaldehyde assimilation: Ribulose monophosphate pathway Glucose-6-phosphate isomerase (EC 5.3.1.9) 
  • PFAM:
    SIS (CL0067) PGI; Phosphoglucose isomerase (PF00342; HMM-score: 82)
    no clan definedELMO_CED12; ELMO/CED-12 family (PF04727; HMM-score: 14.9)
    GT-B (CL0113) Glyco_trans_4_2; Glycosyl transferase 4-like (PF13477; HMM-score: 14.6)
    EDD (CL0245) EIIA-man; PTS system fructose IIA component (PF03610; HMM-score: 13.5)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:
    NWMN_1592(pykA)pyruvate kinase  [1] (data from MRSA252)
    NWMN_2149(rplB)50S ribosomal protein L2  [1] (data from MRSA252)
    NWMN_1151(rplS)50S ribosomal protein L19  [1] (data from MRSA252)
    NWMN_1572(rplT)50S ribosomal protein L20  [1] (data from MRSA252)
    NWMN_2150(rplW)50S ribosomal protein L23  [1] (data from MRSA252)
    NWMN_0641hypothetical protein  [1] (data from MRSA252)

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.116
    • Ymax_pos: 30
    • Cmax: 0.112
    • Cmax_pos: 30
    • Smax: 0.138
    • Smax_pos: 21
    • Smean: 0.096
    • D: 0.108
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MTHIQLDFSKTLEFFGEHELKQQQEIVKSIHKTIHEGTGAGSDFLGWVDLPVDYDKEEFSRIVEASKRIKENSDVLVVIGIGGSYLGARAAIEMLTSSFRNSNEYPEIVFVGNHLSSTYTKELVDYLADKDFSVNVISKSGTTTEPAVAFRLFKQLVEERYGKEEAQKRIFATTDKEKGALKQLATNEGYETFIVPDDVGGRYSVLTAVGLLPIATAGINIEAMMIGAAKAREELSSDKLEENIAYQYATIRNILYAKGYTTEMLINYEPSMQYFNEWWKQLFGESEGKDFKGIYPSSANYTTDLHSLGQYVQEGRRFLFETVVKVNHPKYDITIEKDSDDLDGLNYLAGKTIDEVNTKAFEGTLLAHTDGGVPNMVVNIPQLDEETFGYVVYFFELACAMSGYQLGVNPFNQPGVEAYKQNMFALLGKPGFEDLKKELEERL

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. 1.0 1.1 1.2 1.3 1.4 1.5 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J. Proteome Res.: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)

Relevant publications[edit source | edit]