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PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 06-JUL-2013

Summary[edit | edit source]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_0906 [new locus tag: NWMN_RS05075 ]
  • pan locus tag?: SAUPAN003234000
  • symbol: NWMN_0906
  • pan gene symbol?: tarM
  • synonym:
  • product: glycosyl transferase, group 1 family protein

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: NWMN_0906 [new locus tag: NWMN_RS05075 ]
  • symbol: NWMN_0906
  • product: glycosyl transferase, group 1 family protein
  • replicon: chromosome
  • strand: +
  • coordinates: 1003989..1005470
  • length: 1482
  • essential: unknown other strains

Accession numbers[edit | edit source]

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGAAAAAAATATTTATGATGGTACATGAGTTAGATGTGAATAAAGGTGGTATGACCTCT
    TCGATGTTCAATAGAAGTAAAGAGTTTTATGATGCGGACATACCTGCTGATATTGTTACT
    TTCGATTACAAAGGAAACTATGATGAAATTATTAAAGCTTTGAAAAAACAAGGTAAAATG
    GATCGAAGAACGAAAATGTATAATGTATTTGAGTATTTTAAACAAATTTCAAATAATAAA
    CATTTTAAGTCTAATAAATTGTTATATAAACATATTTCAGAAAGACTAAAAAATACGATT
    GAAATTGAAGAGAGTAAAGGTATTTCAAGATATTTTGATATAACGACTGGTACATATATT
    GCCTACATTAGAAAAAGTAAATCTGAAAAAGTGATTGATTTCTTTAAAGATAATAAACGA
    ATTGAACGGTTTAGTTTTATAGATAATAAAGTGCATATGAAGGAAACATTTAATGTAGAT
    AATAAAGTTTGTTATCAAGTATTTTATGATGAAAAGGGATACCCATATATTTCAAGGAAT
    ATTAATGCTAATAATGGTGCTGTAGGTAAAACTTATGTGTTAGTTAATAAAAAAGAATTT
    AAAAACAATTTAGCACTGTGTGTTTACTATTTAGAAAAACTAATAAAAGATTCTAAAGAT
    AGTATTATGATTTGTGATGGACCAGGGAGTTTTCCAAAAATGTTTAATACAAATCATAAA
    AATGCTCAGAAATATGGCGTTATTCATGTTAATCATCATGAAAATTTCGATGATACGGGT
    GCATTTAAAAAAAGTGAGAAATATATTATTGAGAATGCGAATAAAATTAACGGTGTAATT
    GTATTAACAGAGGCACAAAGATTAGATATTCTTAATCAATTTGATGTAGAAAATATTTTC
    ACTATTAGCAATTTTGTTAAGATACATAATGCTCCAAAACATTTTCAAACTGAAAAAATC
    GTAGGTCATATTTCTAGAATGGTACCAACGAAGCGAATTGATTTGCTTATTGAAGTGGCT
    GAGTTAGTCGTAAAAAAAGATAATGCTGTTAAATTTCATATATATGGAGAAGGATCTGTC
    AAAGATAAAATAGCTAAAATGATTGAAGATAAAAATTTAGAAAGAAATGTTTTTCTTAAA
    GGATATACAACAACTCCACAAAAATGCTTGGAAGATTTTAAATTAGTCGTTTCTACATCT
    CAATATGAAGGTCAAGGGTTAAGTATGATAGAAGCAATGATTTCTAAAAGGCCTGTTGTT
    GCCTTTGACATCAAATACGGACCAAGTGATTTTATAGAAGATAATAAAAATGGTTATTTA
    ATAGAAAACCATAATATTAAGGACATGGCTGATAAAATACTTCAGCTTGTTAATAATGAT
    GTATTAGCAGCGGAGTTTGGTTCGAAAGCGAGAGAAAACATTATAGAAAAATATTCAACG
    GAATCAATATTAGAAAAATGGTTAAATCTTTTCAATAGCTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1482

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: NWMN_0906 [new locus tag: NWMN_RS05075 ]
  • symbol: NWMN_0906
  • description: glycosyl transferase, group 1 family protein
  • length: 493
  • theoretical pI: 9.4155
  • theoretical MW: 57288.5
  • GRAVY: -0.499391

Function[edit | edit source]

  • TIGRFAM:
    Genetic information processing Protein fate Protein modification and repair accessory Sec system glycosylation protein GtfA (TIGR02918; EC 2.4.1.-; HMM-score: 117.2)
    and 15 more
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Glutathione and analogs N-acetyl-alpha-D-glucosaminyl L-malate synthase BshA (TIGR03999; EC 2.4.1.-; HMM-score: 89.5)
    sugar transferase, PEP-CTERM/EpsH1 system associated (TIGR03088; HMM-score: 84.2)
    Metabolism Energy metabolism Biosynthesis and degradation of polysaccharides glycogen synthase, Corynebacterium family (TIGR02149; HMM-score: 35.3)
    colanic acid biosynthesis glycosyltransferase WcaL (TIGR04005; EC 2.4.-.-; HMM-score: 33)
    PEP-CTERM/exosortase A-associated glycosyltransferase, Daro_2409 family (TIGR04063; EC 2.4.-.-; HMM-score: 32.1)
    Metabolism Energy metabolism Biosynthesis and degradation of polysaccharides sucrose synthase (TIGR02470; EC 2.4.1.13; HMM-score: 29.3)
    D-inositol-3-phosphate glycosyltransferase (TIGR03449; EC 2.4.1.250; HMM-score: 29.2)
    glycosyltransferase, GG-Bacteroidales peptide system (TIGR04157; EC 2.4.1.-; HMM-score: 28)
    Metabolism Energy metabolism Biosynthesis and degradation of polysaccharides glycogen/starch synthase, ADP-glucose type (TIGR02095; EC 2.4.1.21; HMM-score: 24.8)
    sucrose-phosphate synthase, putative, glycosyltransferase domain (TIGR02472; EC 2.4.1.14; HMM-score: 24.7)
    sucrose phosphate synthase (TIGR02468; EC 2.4.1.14; HMM-score: 19.3)
    Unknown function Enzymes of unknown specificity glycosyltransferase, MSMEG_0565 family (TIGR04047; HMM-score: 16.8)
    sugar transferase, PEP-CTERM/EpsH1 system associated (TIGR03087; HMM-score: 16.4)
    putative glycosyltransferase, TIGR04348 family (TIGR04348; EC 2.4.1.-; HMM-score: 14.8)
    RNA polymerase sigma factor, SigM family (TIGR02950; HMM-score: 12.8)
  • TheSEED: data available for COL, NCTC8325, USA300_FPR3757
  • PFAM:
    GT-B (CL0113) Glycos_transf_1; Glycosyl transferases group 1 (PF00534; HMM-score: 113.4)
    and 4 more
    Glyco_trans_1_4; Glycosyl transferases group 1 (PF13692; HMM-score: 88.9)
    Glyco_trans_1_2; Glycosyl transferases group 1 (PF13524; HMM-score: 32.3)
    no clan defined DUF4116; Domain of unknown function (DUF4116) (PF13475; HMM-score: 13.9)
    DUF3695; Protein of unknown function (DUF3695) (PF12494; HMM-score: 12.4)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.010241
    • TAT(Tat/SPI): 0.000295
    • LIPO(Sec/SPII): 0.001551
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MKKIFMMVHELDVNKGGMTSSMFNRSKEFYDADIPADIVTFDYKGNYDEIIKALKKQGKMDRRTKMYNVFEYFKQISNNKHFKSNKLLYKHISERLKNTIEIEESKGISRYFDITTGTYIAYIRKSKSEKVIDFFKDNKRIERFSFIDNKVHMKETFNVDNKVCYQVFYDEKGYPYISRNINANNGAVGKTYVLVNKKEFKNNLALCVYYLEKLIKDSKDSIMICDGPGSFPKMFNTNHKNAQKYGVIHVNHHENFDDTGAFKKSEKYIIENANKINGVIVLTEAQRLDILNQFDVENIFTISNFVKIHNAPKHFQTEKIVGHISRMVPTKRIDLLIEVAELVVKKDNAVKFHIYGEGSVKDKIAKMIEDKNLERNVFLKGYTTTPQKCLEDFKLVVSTSQYEGQGLSMIEAMISKRPVVAFDIKYGPSDFIEDNKNGYLIENHNIKDMADKILQLVNNDVLAAEFGSKARENIIEKYSTESILEKWLNLFNS

Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

Expression & Regulation[edit | edit source]

Regulation[edit | edit source]

  • regulator:

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]

Solmaz Sobhanifar, Liam James Worrall, Robert J Gruninger, Gregory A Wasney, Markus Blaukopf, Lars Baumann, Emilie Lameignere, Matthew Solomonson, Eric D Brown, Stephen G Withers, Natalie C J Strynadka
Structure and mechanism of Staphylococcus aureus TarM, the wall teichoic acid α-glycosyltransferase.
Proc Natl Acad Sci U S A: 2015, 112(6);E576-85
[PubMed:25624472] [WorldCat.org] [DOI] (I p)