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PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 06-JUL-2013

Summary[edit | edit source]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_1015 [new locus tag: NWMN_RS05750 ]
  • pan locus tag?: SAUPAN001675000
  • symbol: NWMN_1015
  • pan gene symbol?:
  • synonym:
  • product: phage terminase large subunit

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: NWMN_1015 [new locus tag: NWMN_RS05750 ]
  • symbol: NWMN_1015
  • product: phage terminase large subunit
  • replicon: chromosome
  • strand: +
  • coordinates: 1115916..1117193
  • length: 1278
  • essential: unknown other strains

Accession numbers[edit | edit source]

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    ATGACGAAAGTTAAATTAAACTTTAACAAACCATCTAATGTTTTCAATAGAAACATATTC
    GAAATACTAACCAATTACGATAACTTCACTGAAGTACATTACGGTGGAGGTTCGAGCGGT
    AAGTCTCACGGCGTTATACAAAAAGTTGTACTTAAAGCATTGCAAGACTGGAAATATCCT
    AGGCGTATACTATGGCTTAGAAAAGTCCAATCAACAATTAAAGATAGTTTATTCGAAGAT
    GTCAAAGATTGTTTGATAAACTTCGGTATTTGGGACATGTGCCTTTGGAATAAGACTGAT
    AACAAAGTTGAATTGCCAAACGGCGCAGTTTTTTTGTTTAAAGGATTAGATAACCCAGAG
    AAAATAAAGTCGATAAAAGGCATATCAGACATAGTCATGGAAGAAGCGTCTGAATTCACA
    CTAAATGATTACACGCAATTAACGTTGCGTTTGAGGGAGCGTAAACACGTGAATAAGCAA
    ATATTTTTGATGTTTAACCCAGTATCTAAACTGAATTGGGTTTATAAGTATTTCTTTGAA
    CATGGTGAACCAATGGAAAATGTCATGATTAGACAATCTAGTTATCGAGATAATAAGTTT
    CTTGATGAAATGACACGACAAAACTTAGAGTTGTTAGCAAATCGTAATCCAGCATATTAC
    AAAATTTATGCGTTAGGTGAATTTGCTACACTAGACAAATTGGTTTTCCCTAAGTATGAA
    AAACGTTTAATAAATAAAGATGAGTTAAGACATTTACCTTCTTATTTTGGATTGGACTTT
    GGCTACGTTAATGATCCTAGTGCTTTTATACATTCTAAAATAGATGTAAAGAAAAAGAAG
    TTATACATCATTGAAGAGTATGTTAAACAAGGTATGCTGAATGATGAAATAGCTAATGTC
    ATAAAGCAACTTGGTTATGCTAAAGAAGAAATTACAGCAGATAGTGCAGAACAAAAAAGT
    ATAGCTGAATTAAGGAATCTAGGGCTTAAAAGGATTTTACCAACCAAAAAAGGGAAGGGC
    TCGGTTGTACAAGGGTTACAATTCTTAATGCAATTTGAAATCATTGTTGATGAACGTTGT
    TTCAAGACTATTGAAGAGTTTGACAACTACACATGGCAAAAGGACAAAGATACAGGTGAA
    TATACCAATGAACCAGTAGATACATACAATCATTGTATCGATTCGTTGCGTTATTCAGTG
    GAACGATTCTACAGACCGGTTAGAAAACGCACAAATGTCAGTTCGAAAGTTGACACAATA
    AAATCTCTAGGATTATAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1278

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: NWMN_1015 [new locus tag: NWMN_RS05750 ]
  • symbol: NWMN_1015
  • description: phage terminase large subunit
  • length: 425
  • theoretical pI: 9.34345
  • theoretical MW: 49912
  • GRAVY: -0.512706

Function[edit | edit source]

  • TIGRFAM:
    Genetic information processing Mobile and extrachromosomal element functions Prophage functions phage terminase, large subunit, PBSX family (TIGR01547; HMM-score: 394.5)
  • TheSEED: data available for NCTC8325
  • PFAM:
    P-loop_NTPase (CL0023) Terminase_3; Phage terminase large subunit (PF04466; HMM-score: 203.6)
    and 4 more
    RNase_H (CL0219) Terminase_3C; Terminase RNAseH like domain (PF17288; HMM-score: 119.3)
    P-loop_NTPase (CL0023) Terminase_6; Terminase-like family (PF03237; HMM-score: 29.9)
    RNase_H (CL0219) Terminase_6C; Terminase RNaseH-like domain (PF17289; HMM-score: 16.2)
    UBA (CL0214) UBA_3; Fungal ubiquitin-associated domain (PF09288; HMM-score: 12.7)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.005572
    • TAT(Tat/SPI): 0.000246
    • LIPO(Sec/SPII): 0.000493
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MTKVKLNFNKPSNVFNRNIFEILTNYDNFTEVHYGGGSSGKSHGVIQKVVLKALQDWKYPRRILWLRKVQSTIKDSLFEDVKDCLINFGIWDMCLWNKTDNKVELPNGAVFLFKGLDNPEKIKSIKGISDIVMEEASEFTLNDYTQLTLRLRERKHVNKQIFLMFNPVSKLNWVYKYFFEHGEPMENVMIRQSSYRDNKFLDEMTRQNLELLANRNPAYYKIYALGEFATLDKLVFPKYEKRLINKDELRHLPSYFGLDFGYVNDPSAFIHSKIDVKKKKLYIIEEYVKQGMLNDEIANVIKQLGYAKEEITADSAEQKSIAELRNLGLKRILPTKKGKGSVVQGLQFLMQFEIIVDERCFKTIEEFDNYTWQKDKDTGEYTNEPVDTYNHCIDSLRYSVERFYRPVRKRTNVSSKVDTIKSLGL

Experimental data[edit | edit source]

  • experimentally validated:
  • protein localization:
  • quantitative data / protein copy number per cell:
  • interaction partners:

Expression & Regulation[edit | edit source]

Regulation[edit | edit source]

  • regulator:

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]