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Pangenome04-0298108BA0217611819-9771193COLECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50N315NCTC8325NewmanRF122ST398T0131TCH60TW20USA300_FPR3757USA300_TCH1516VC40
NCBI: 03-AUG-2016

Summary[edit source | edit]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_02049
  • pan locus tag?: SAUPAN001675000
  • symbol: SAOUHSC_02049
  • pan gene symbol?:
  • synonym:
  • product: PBSX family phage terminase large subunit

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SAOUHSC_02049
  • symbol: SAOUHSC_02049
  • product: PBSX family phage terminase large subunit
  • replicon: chromosome
  • strand: -
  • coordinates: 1948782..1950059
  • length: 1278
  • essential: no DEG

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    ATGACGAAAGTTAAATTAAACTTTAACAAACCGTCTAATGTTTTCAATAGAAACATATTC
    GAAATACTAACCAATTACGATAACTTCACTGAAGTACATTACGGTGGAGGTTCGAGCGGT
    AAGTCTCACGGCGTTATACAAAAAGTTGTACTCAAAGCATTGCAAGATTGGAAATATCCT
    AGGCGTATACTGTGGCTTAGAAAAGTACAATCAACAATTAAAGATAGTTTGTTCGAAGAT
    GTTAAAGATTGTTTGATAAACTTTGGTATTTGGGACATGTGCCTTTGGAATAAGACTGAT
    AACAAAGTTGAATTGCCAAACGGCGCAGTTTTTTTGTTTAAAGGATTAGATAACCCAGAG
    AAAATAAAGTCGATAAAAGGCATATCAGACATAGTCATGGAAGAAGCGTCTGAATTCACA
    CTAAATGATTACACGCAATTAACGTTGCGTTTGAGGGAGCGTAAACACGTGAATAAGCAA
    ATATTTTTGATGTTTAACCCAGTATCTAAACTGAATTGGGTTTATAAGTATTTCTTTGAA
    CATGGTGAACCAATGGAAAATGTCATGATTAGACAATCTAGTTATCGAGATAATAAGTTT
    CTTGATGAAATGACACGACAAAACTTAGAGTTGTTAGCAAATCGTAATCCAGCATATTAC
    AAAATTTATGCGTTAGGTGAATTTGCTACACTAGACAAATTGGTTTTCCCTAAGTATGAA
    AAACGTTTAATAAATAAAGATGAGTTAAGACATTTACCTTCTTATTTTGGATTGGACTTT
    GGCTACGTTAATGATCCTAGTGCTTTTATACATTCTAAAATAGATGTAAAGAAAAAGAAG
    TTATACATCATTGAAGAGTATGTTAAACAAGGTATGCTGAATGATGAAATAGCTAATGTC
    ATAAAGCAACTTGGTTATGCTAAAGAAGAAATTACAGCAGATAGTGCAGAACAAAAAAGT
    ATAGCTGAATTAAGGAATCTAGGGCTTAAAAGGATTTTACCAACCAAAAAAGGGAAGGGC
    TCGGTTGTACAAGGGTTACAATTCTTAATGCAATTTGAAATCATTGTTGATGAACGTTGT
    TTCAAGACTATTGAAGAGTTTGACAACTACACATGGCAAAAGGACAAAGATACAGGTGAA
    TATACCAATGAACCAGTAGATACATACAATCATTGTATCGATTCGTTGCGTTATTCAGTG
    GAACGATTCTACAGACCGGTTAGAAAACGCACAAATCTCAGTTCGAAAGTTGACACAATA
    AAATCTCTAGGATTATAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1278

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SAOUHSC_02049
  • symbol: SAOUHSC_02049
  • description: PBSX family phage terminase large subunit
  • length: 425
  • theoretical pI: 9.34345
  • theoretical MW: 49926.1
  • GRAVY: -0.513647

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    Genetic information processingMobile and extrachromosomal element functionsProphage functionsphage terminase, large subunit, PBSX family (TIGR01547; HMM-score: 394.3)
  • TheSEED:  
    Phages, Prophages, Transposable elementsPhages, ProphagesPhage head and packaging Phage terminase, large subunit 
  • PFAM:
    P-loop_NTPase (CL0023) Terminase_3; Phage terminase large subunit (PF04466; HMM-score: 203.6)
    RNase_H (CL0219) Terminase_3C; Terminase RNAseH like domain (PF17288; HMM-score: 119.3)
    P-loop_NTPase (CL0023) Terminase_6; Terminase-like family (PF03237; HMM-score: 29.9)
    RNase_H (CL0219) Terminase_6C; Terminase RNaseH-like domain (PF17289; HMM-score: 16.2)
    UBA (CL0214) UBA_3; Fungal ubiquitin-associated domain (PF09288; HMM-score: 12.6)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.168
    • Ymax_pos: 11
    • Cmax: 0.112
    • Cmax_pos: 41
    • Smax: 0.336
    • Smax_pos: 1
    • Smean: 0.271
    • D: 0.208
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MTKVKLNFNKPSNVFNRNIFEILTNYDNFTEVHYGGGSSGKSHGVIQKVVLKALQDWKYPRRILWLRKVQSTIKDSLFEDVKDCLINFGIWDMCLWNKTDNKVELPNGAVFLFKGLDNPEKIKSIKGISDIVMEEASEFTLNDYTQLTLRLRERKHVNKQIFLMFNPVSKLNWVYKYFFEHGEPMENVMIRQSSYRDNKFLDEMTRQNLELLANRNPAYYKIYALGEFATLDKLVFPKYEKRLINKDELRHLPSYFGLDFGYVNDPSAFIHSKIDVKKKKLYIIEEYVKQGMLNDEIANVIKQLGYAKEEITADSAEQKSIAELRNLGLKRILPTKKGKGSVVQGLQFLMQFEIIVDERCFKTIEEFDNYTWQKDKDTGEYTNEPVDTYNHCIDSLRYSVERFYRPVRKRTNLSSKVDTIKSLGL

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet.: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

Relevant publications[edit source | edit]