From AureoWiki
Jump to: navigation, search
Pangenome04-0298108BA0217611819-9771193COLECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50N315NCTC8325NewmanRF122ST398T0131TCH60TW20USA300_FPR3757USA300_TCH1516VC40
NCBI: 03-AUG-2016

Summary[edit source | edit]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_02028
  • pan locus tag?: SAUPAN001699000
  • symbol: SAOUHSC_02028
  • pan gene symbol?:
  • synonym:
  • product: phiETA ORF57-like protein

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SAOUHSC_02028
  • symbol: SAOUHSC_02028
  • product: phiETA ORF57-like protein
  • replicon: chromosome
  • strand: -
  • coordinates: 1931080..1932903
  • length: 1824
  • essential: no DEG

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGGTAGTAGATAATTTTTCGAAAGATGATAACTTAATCGAGTTACAAACAACATCACAA
    TATAATCCGGTTATTGACACAAACATCAGTTTCTATGAATCAGATAGAGGAACTGGTGTT
    TTAAATTTTGCAGTAACTAAGAATAACAGACCCTTATCTATAAGTTCTGAACATGTTAAA
    ACATCTATCGTGTTAAAAACCGATGATTATAACGTAGATAGAGGCGCTTATATTTCAGAC
    GAATTAACGATAGTAGACGCAATTAATGGGCGTTTGCAGTATGTGATACCGAATGAATTT
    TTAAAACATTCAGGCAAGGTGCATGCTCAGGCATTCTTTACACAAAACGGGAGTAATAAT
    GTTGTTGTTGAACGTCAATTTAGCTTCAATATTGAAAATGATTTAGTTAGTGGGTTTGAT
    GGTATAACAAAGCTTGTTTATATCAAATCTATTCAAGATACTATCGAAGCTGTCGGTAAA
    GATTTTAACCAATTAAAGCAAAATATGGCTGATACACAAACGTTAATAGCAAAAGTGAAT
    GATAGTGCGACAAAAGGCATTCAACAAATCGAAATCAAGCAAAACGAAGCTATACAAGCT
    ATTACTGCGACGCAAACTAGTGCAACACAAGCTGTTACAGCTGAATTCGATAAAATAGTT
    GAAAAAGAGCAAGCGATTTTTGAACGTGTTAACGAAGTTGAACAACAAATCAATGGCGCT
    GACCTTGTTAAAGGTAATTCAACAACGAATTGGCAAAAGTCTAAACTTACAGATGATTAC
    GGTAAAGCAATTGAATCGTATGAGCAGTCCATAGATAGCGTTTTAAGCGCAGTTAACACA
    TCTAGGATTATTCATATTACTAATGCAACAGATGCGCCAGAAAAGACGGATATAGGCACG
    TTAGAGAAGCCTGGACAAGATGGTGTTGATGACGGTTCTTCGTTCGATGAATCAACTTAT
    ACATCAAGCAAATCTGGTGTGTTAGTTGTTTATGTTGTTGATAATAATACTGCTCGTGCA
    ACATGGTACCCAGACGATTCAAACGATGAGTACACAAAATACAAAATCTACGGCACATGG
    TACCCGTTTTATAAAAAGAATGATGGAAACTTAACTAAGCAATTTGTTGAAGAAACGTCT
    AACAACGCTTTAAATCAAGCTAAGCAGTATGTAGATGATAAATTCGGAACAACGAGCTGG
    CAACAACATAAGATGACAGAGGCGAATGGTCAATCAATTCAAGTTAACTTAAATAATGCG
    CAAGGCGATTTGGGATATTTAACTGCTGGTAATTACTATGCAACAAGAGTGCCGGATTTA
    CCAGGTAGCGTTGAAAGTTATGAGGGTTATTTATCGGTATTCGTTAAAGATGATACAAAC
    AAGCTATTTAACTTCACACCTTATAACTCTAAAAAGATTTACACACGATCAATCACAAAC
    GGCAGACTTGAGCAACAGTGGACAGTTCCTAATGAACATAAATCAACGGTATTGTTCGAC
    GGTGGCGCAAATGGTGTAGGTACAACAATCAATCTAACTGAACCGTACACAAACTATTCT
    ATTTTGTTGGTAAGTGGAACTTATCCAGGTGGCGTTATTGAGGGATTCGGACTAACCGCA
    TTACCTAACGCGATTCAATTGAGTAAAGCGAATGTAGTTGACTCAGACGGCAACGGTGGC
    GGTATTTATGAGTGCTTACTATCCAAAACAAGTAGCACTACTTTAAGAATAGATAACGAT
    GTGTACTTTGATTTAGGTAAAACATCAGGTTCTGGAGCGAATGCCAACAAAGTTACTATA
    ACTAAAATTATGGGGTGGAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1740
    1800
    1824

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SAOUHSC_02028
  • symbol: SAOUHSC_02028
  • description: phiETA ORF57-like protein
  • length: 607
  • theoretical pI: 4.53212
  • theoretical MW: 66986.4
  • GRAVY: -0.497364

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
  • TheSEED:  
    FIG01108548: hypothetical protein 
  • PFAM:
    no clan definedDUF2479; Domain of unknown function (DUF2479) (PF10651; HMM-score: 122.7)
    SNARE-fusion (CL0445) Syntaxin_2; Syntaxin-like protein (PF14523; HMM-score: 16.5)
    no clan definedDUF1664; Protein of unknown function (DUF1664) (PF07889; HMM-score: 13.8)
    DUF1477; Protein of unknown function (DUF1477) (PF07346; HMM-score: 12.7)
    Collagen_mid; Bacterial collagen, middle region (PF15984; HMM-score: 11.4)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 2.5
    • Cytoplasmic Membrane Score: 2.5
    • Cellwall Score: 2.5
    • Extracellular Score: 2.5
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.106
    • Ymax_pos: 27
    • Cmax: 0.109
    • Cmax_pos: 22
    • Smax: 0.137
    • Smax_pos: 26
    • Smean: 0.099
    • D: 0.103
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MVVDNFSKDDNLIELQTTSQYNPVIDTNISFYESDRGTGVLNFAVTKNNRPLSISSEHVKTSIVLKTDDYNVDRGAYISDELTIVDAINGRLQYVIPNEFLKHSGKVHAQAFFTQNGSNNVVVERQFSFNIENDLVSGFDGITKLVYIKSIQDTIEAVGKDFNQLKQNMADTQTLIAKVNDSATKGIQQIEIKQNEAIQAITATQTSATQAVTAEFDKIVEKEQAIFERVNEVEQQINGADLVKGNSTTNWQKSKLTDDYGKAIESYEQSIDSVLSAVNTSRIIHITNATDAPEKTDIGTLEKPGQDGVDDGSSFDESTYTSSKSGVLVVYVVDNNTARATWYPDDSNDEYTKYKIYGTWYPFYKKNDGNLTKQFVEETSNNALNQAKQYVDDKFGTTSWQQHKMTEANGQSIQVNLNNAQGDLGYLTAGNYYATRVPDLPGSVESYEGYLSVFVKDDTNKLFNFTPYNSKKIYTRSITNGRLEQQWTVPNEHKSTVLFDGGANGVGTTINLTEPYTNYSILLVSGTYPGGVIEGFGLTALPNAIQLSKANVVDSDGNGGGIYECLLSKTSSTTLRIDNDVYFDLGKTSGSGANANKVTITKIMGWK

Experimental data[edit source | edit]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  2. Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-3661
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  3. Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet.: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

Relevant publications[edit source | edit]