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Pangenome04-0298108BA0217611819-9771193COLECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50N315NCTC8325NewmanRF122ST398T0131TCH60TW20USA300_FPR3757USA300_TCH1516VC40
NCBI: 03-AUG-2016

Summary[edit source | edit]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_02033
  • pan locus tag?: SAUPAN001695000
  • symbol: SAOUHSC_02033
  • pan gene symbol?:
  • synonym:
  • product: phage tape measure protein

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SAOUHSC_02033
  • symbol: SAOUHSC_02033
  • product: phage tape measure protein
  • replicon: chromosome
  • strand: -
  • coordinates: 1937698..1941165
  • length: 3468
  • essential: no DEG

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    GTGACGGAATATAAAATTAAAGCGACTATTGAAGCTAGTGTAGCCAAATTCAAAAGGCAA
    ATTGATAGTGCGGTTAAGTCTGTGCAAAGATTTAAACGAGTAGCAGATCAAACTAAAGAT
    GTTGAATTAAACGCTAACGATAAAAATTTACAAAAAACTATCAAAGTTGCTAAAAAGTCT
    TTAGATGCCTTTAGTAACAAAAATGTAAAAGCTAAATTAGATGCTAGTATACAAGATTTA
    CAACAAAAGGTACTAGAATCGAATTTTGAACTAGACAAACTAAACTCTAAAGAAGTTACA
    CCAGAAGTTAAGTTGCAAAAACAAAAGTTGATTAAAGATATCGCTGAAACAGAAGCTAAA
    TTATCAGAATTAGAAAAGAAACGTGTCAATATTGACGTCAATGCAGATAACAGTAAATTC
    AATCGAGTGTTAAAAGTATCTAAAGCTAGTCTCGAAGCATTAAATAGGTCTAAAGCCAAA
    GCTATTATAGACGTGGACAATGGTGTTGCTAACTCTAAAATCAAACGTACTAAAGAAGAA
    CTTAAAAGTATTCCGAACAAAACTAGATCTCGACTTGATGTAGATACAGGGCTTTCTATA
    CCAACTATTTATGCGTTTAAAAAATCATTAGACGCATTGCCGAACAAAAAAACAACAAAG
    GTAGATGTCGATACTAATGGTTTAAAGAAAGCTTATGCCTACATAATAAAAGCAAATGAC
    AATTTTCAAAGACAGATGGGGAATTTAGCTAATATGTTCCGTGTGTTCGGTACTGTAGGT
    TCTAATATGGTTGGTGGATTACTTACATCATCTTTTAGTATCTTAATACCTGTAATAGCG
    AGCGTAGTACCTGTAGTATTTGCGCTATTAAACGCTATCAAAGTGTTAACTGGTGGTGTA
    CTTGCTTTAGGTGGTGCCGTAGCAATAGCGGGAGCAGGATTTGTAGCGTTTGGCGCAATG
    GCTATCAGCGCTATAAAGATGCTTAATGATGGCACTTTACAAGCTAGCTCAGCAACAAAC
    GAATACAAAAAAGCGTTAGATGGCGTAAAGTCAGCATGGACTGATATTATAAAGCAAAAT
    CAATCCGCTATCTTCACAACTCTTGCAAATGGTTTAAATACTGTTAAAACTGCAATGCAG
    AGCTTACAACCGTTTTTTAGTGGTATTTCAAGAGGAATGGAAGAAGCGTCTCAAAGCGTG
    CTTAAATGGGCTGAAAATAGCAGTGTAGCTTCAAGATTCTTTAATATGATGAATACAACG
    GGTGTTTCGGTATTTAACAAGCTATTAAGTGCTGCAGGTGGTTTTGGTGACGGATTAGTC
    AATGTATTCACGCAATTAGCACCACTGTTTCAATGGTCGGCTGATTGGTTGGATAGATTA
    GGTCAATCTTTCTCTAACTGGGCTAATAGTGCAGCTGGAGAAAATTCGATAACTCGTTTT
    ATTGAATACACAAAAACAAACTTACCTATCATTGGTAATATTTTCAAAAATGTTTTCGTT
    GGAATTAACAATTTGATGAATGCATTCAGCGGATCATCAACTGGCATATTCCAATCTCTT
    GAACAAATGACAGCTAAGTTTAGGGAATGGTCTGAACAAGTAGGACAATCTCAAGGGTTT
    AAAGACTTTGTCAGTTATATACAAACAAATGGACCACTAATAATGCAATTGATTGGAAAC
    ATCGCAAGAGGATTAGTTGCATTCGCAACAGCAATGGCTCCTATAGCTAGTGCAGTATTA
    CGCGTTGCAGTTGCAATAACTGGTTGGATAGCTAACTTGTTTGAGGCGCATCCAGCTACA
    GCACAATTAGTTGGTGTCATTATAACTTTAGTTGGTGCATTTAGATTTTTAATACCGATT
    ATTCTTGCTGTATCTAACTTTATGGGTGGCGGATTAATAGGTAGAATCATTGCATTAGTA
    AGTAAGTTCGGTTTATTAAGAGCGGGATTAACAATTTTAAAAGGTGCGTTCATGTTATTA
    AAAGGACCATTAAAAATTATATCAGTTATATTCCAATTGTTATTCGGTAAGATTGGATTA
    ATTAGAAATGCTATCACAGGACTAGTAACTGTGTTTGGTATTTTAGGCGGTCCAATAACA
    ATAGTAATTGGTGTAATTGCTGCATTAATAGCTATATTCGTTTTATTGTGGAATAAAAAT
    GAAGGATTCAGAAACTTTATTATAAATGCTTGGAATGCGATAAAAACGTTTATGGTTAAT
    GTTTGGAATGTATTAAAAGCTGTAGCTTCGGTTGTATGGAATGCTATTTTAACAGCTATC
    ACTACAGCAGTATCGAATGTTTACAATTTTATAATGATTGTTTGGAATCAAATAGTCGCT
    TATTTACAAGGGCTATGGAATGGAATTATCGCTATTGCAACAACAGTATGGAACCTTTTA
    GTTACAATCATTACAACTGTTTTCACGACGATAATGACAATAGTTATGACGATATGGACA
    GCTATTTGGACGTTCTTAAGTACAATCTGGAATACGATAATTACAATCGCTACTACGATT
    TGGAATTTGTTAGTCACTGTAATAACTACAGTGTTTACCACAATTATGACTATCGCAATA
    ACAATTTGGAACGCTATTTGGACGTTCTTACAAACGTTGTGGAACACTATAGTTACTGTG
    GCAACTAAGGTTTGGAACGCTATCACTACAGCTATATCTACTGCGTTACAAGCGGCATGG
    AGTTTTATTTCTAATATATGGAATACGATTTGGAGTTTCTTATCTGGTATATTAACGACA
    ATTTGGAATAAAGTTGTAAGCATATTCACACAAGTTGTTTCAACTATATCAGACAAAATG
    TCTCAAGCTTGGAACTTCATTGTCACTAAAGGTATGCAATGGGTATCTACTATAACAAGT
    ACGCTAATTAACTTTGTTAATAGAGTTGTTCAAGGATTCGTTAATGTTGTAAACAAAGTT
    AGTCAAGGTATGACAAATGCAGTAAATAAAGTTAAAAGCTTTGTGGATGACTTTGTATCA
    GCAGGTGCTGATATGATCCGTGGTTTGATGAGAGGTATTGGTAATATGGCTAGAGACTTA
    GCTGAAAAAGCAGCTAGTGTAGCAAAAGGTGCTTTAAATGCAGCCAAAAGAGCGCTAGGT
    ATTCACTCACCTTCACGTGAATTCATGGATGTTGGTATGTATTCAATGTTAGGTTTCGTT
    AAAGGTATAGATAATCATTCAAGTAAAGTTATCCGTAATGTTTCTAATGTTGCAGATAAA
    GTAGTTGATGCATTTCAACCTACATTAAACGCACCTGACATTTCTAGTATTACAGGAAAC
    TTAAGTAATTTAGGTGGAAATATAAATGCGCAAGTACAACACACACATTCTATTGAAACA
    TCACCGAACATGAAAACTGTTAAAGTTGAATTCGATGTCAATAACGATGCGCTTACTAGT
    ATTGTTAACGGCAGAAATGCTAAACGCAATTCTGAGTATTACTTATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
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    1920
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    2040
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    2160
    2220
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    2400
    2460
    2520
    2580
    2640
    2700
    2760
    2820
    2880
    2940
    3000
    3060
    3120
    3180
    3240
    3300
    3360
    3420
    3468

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SAOUHSC_02033
  • symbol: SAOUHSC_02033
  • description: phage tape measure protein
  • length: 1155
  • theoretical pI: 10.6508
  • theoretical MW: 125813
  • GRAVY: 0.325368

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
  • TheSEED:  
    Phage tail proteins 2 Tape measure protein [SA bacteriophages 11, Mu50B] 
  • PFAM:
    HTH (CL0123) F-112; F-112 protein (PF09645; HMM-score: 15.7)
    Traffic (CL0147) Sec20; Sec20 (PF03908; HMM-score: 6.5)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 14
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular Possibility: 0.17
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.152
    • Ymax_pos: 36
    • Cmax: 0.105
    • Cmax_pos: 36
    • Smax: 0.299
    • Smax_pos: 30
    • Smean: 0.176
    • D: 0.162
  • predicted transmembrane helices (TMHMM): 13

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MTEYKIKATIEASVAKFKRQIDSAVKSVQRFKRVADQTKDVELNANDKNLQKTIKVAKKSLDAFSNKNVKAKLDASIQDLQQKVLESNFELDKLNSKEVTPEVKLQKQKLIKDIAETEAKLSELEKKRVNIDVNADNSKFNRVLKVSKASLEALNRSKAKAIIDVDNGVANSKIKRTKEELKSIPNKTRSRLDVDTGLSIPTIYAFKKSLDALPNKKTTKVDVDTNGLKKAYAYIIKANDNFQRQMGNLANMFRVFGTVGSNMVGGLLTSSFSILIPVIASVVPVVFALLNAIKVLTGGVLALGGAVAIAGAGFVAFGAMAISAIKMLNDGTLQASSATNEYKKALDGVKSAWTDIIKQNQSAIFTTLANGLNTVKTAMQSLQPFFSGISRGMEEASQSVLKWAENSSVASRFFNMMNTTGVSVFNKLLSAAGGFGDGLVNVFTQLAPLFQWSADWLDRLGQSFSNWANSAAGENSITRFIEYTKTNLPIIGNIFKNVFVGINNLMNAFSGSSTGIFQSLEQMTAKFREWSEQVGQSQGFKDFVSYIQTNGPLIMQLIGNIARGLVAFATAMAPIASAVLRVAVAITGWIANLFEAHPATAQLVGVIITLVGAFRFLIPIILAVSNFMGGGLIGRIIALVSKFGLLRAGLTILKGAFMLLKGPLKIISVIFQLLFGKIGLIRNAITGLVTVFGILGGPITIVIGVIAALIAIFVLLWNKNEGFRNFIINAWNAIKTFMVNVWNVLKAVASVVWNAILTAITTAVSNVYNFIMIVWNQIVAYLQGLWNGIIAIATTVWNLLVTIITTVFTTIMTIVMTIWTAIWTFLSTIWNTIITIATTIWNLLVTVITTVFTTIMTIAITIWNAIWTFLQTLWNTIVTVATKVWNAITTAISTALQAAWSFISNIWNTIWSFLSGILTTIWNKVVSIFTQVVSTISDKMSQAWNFIVTKGMQWVSTITSTLINFVNRVVQGFVNVVNKVSQGMTNAVNKVKSFVDDFVSAGADMIRGLMRGIGNMARDLAEKAASVAKGALNAAKRALGIHSPSREFMDVGMYSMLGFVKGIDNHSSKVIRNVSNVADKVVDAFQPTLNAPDISSITGNLSNLGGNINAQVQHTHSIETSPNMKTVKVEFDVNNDALTSIVNGRNAKRNSEYYL

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet.: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

Relevant publications[edit source | edit]