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NCBI: 06-JUL-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_1228 [new locus tag: NWMN_RS06940 ]
  • pan locus tag?: SAUPAN003680000
  • symbol: NWMN_1228
  • pan gene symbol?:
  • synonym:
  • product: hypothetical protein

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: NWMN_1228 [new locus tag: NWMN_RS06940 ]
  • symbol: NWMN_1228
  • product: hypothetical protein
  • replicon: chromosome
  • strand: -
  • coordinates: 1352364..1353419
  • length: 1056
  • essential: unknown other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    ATGTTGATATTAGTTTTAGGGGGAAATGTAGTGATTTCATTCGAAAATGATTATTTAGAA
    GGTGCTCATGAAAAAGTATTAAAACGATTAGTAGATACAAATCTTGTACAAGCTTCGGGA
    TATGGCTTTGATCAATTCACAGCTCAGGCAATTGAAAAGATTAAAGATACAATTGACTGT
    CCAAATGCTACAATTCGTTTTTTAGTAGGTGGAACACAAACCAATCAGGTTGTTATTAAT
    TCAATGTTAGAAAGTTACGAAGGGGTCATTTCAGCAGACACTGGTCATGTTGCTGTGCAT
    GAGGGCGGTGCAATTGAATATAGTGGACATAAAGTAATTACTATACCCTCTAAGGAAGGA
    AAAGTTAGTGCTTCAGATGTTGAAACTTACATGGAGACATTTAAAAGTGATTTTAAAAAG
    GACCATATGGTATTCCCAGGAATGGTATATATTTCTCATCCTACAGAATATGGCACATTA
    TACTCTAAATCAGAATTGGAAGAGTTATGCAAAGTATGTAAGCAATATCAGCTTCCATTA
    TTTATGGATGGTGCACGATTAGGGTATGGATTGATGAGTGACCAATCAGATATGACTATT
    AAAGACATCGCTAAATATTGTGATGTATTTTATATAGGTGGTACGAAGATAGGTGCATTA
    TGCGGGGAAGCCATTGTTTTTACGAAAAATAATGAACCGAAACAATTTACCACTCGAATC
    AAGCATCACGGTGCCCTTTTAGCAAAAGGACGGTTAACAGGCATACAATTTTTAGAATTA
    TTTACGGACAATTTATATTTTAATATAAGCAGACATGCTATCGAAATGGCAAATAAGATG
    AAAGACGGGTTTAAAAATAAAGGCTATCGTCTTTACTTTGATTCTCCAACCAATCAACAA
    TTTTTTATTTTAAGTAACGAGAAAATAGCAGAGTTAGAACAAAAAGTTAAATTTGCTGTT
    TGGGAAAAATACGATGATCAACATCGTGTTGTTAGATTCGCAACCAGTTGGGCGACAACC
    GAAGAAAATTTAAATAAACTATTAGAATTAATCTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1056

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: NWMN_1228 [new locus tag: NWMN_RS06940 ]
  • symbol: NWMN_1228
  • description: hypothetical protein
  • length: 351
  • theoretical pI: 6.18837
  • theoretical MW: 39612
  • GRAVY: -0.216239

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    O-phospho-L-seryl-tRNA:Cys-tRNA synthase (TIGR02539; EC 2.5.1.73; HMM-score: 22.2)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersOthertyrosine decarboxylase MnfA (TIGR03812; EC 4.1.1.25; HMM-score: 20.2)
    cysteine desulfurase NifS (TIGR03402; EC 2.8.1.7; HMM-score: 16.5)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersOthercoenzyme PQQ biosynthesis protein PqqD (TIGR03859; HMM-score: 12)
  • TheSEED:  
    Amino Acids and DerivativesAlanine, serine, and glycineGlycine Biosynthesis Low-specificity L-threonine aldolase (EC 4.1.2.48) 
    Lysine, threonine, methionine, and cysteineThreonine degradation Low-specificity L-threonine aldolase (EC 4.1.2.48) 
  • PFAM:
    PLP_aminotran (CL0061) Beta_elim_lyase; Beta-eliminating lyase (PF01212; HMM-score: 100)
    Aminotran_1_2; Aminotransferase class I and II (PF00155; HMM-score: 25.2)
    Aminotran_5; Aminotransferase class-V (PF00266; HMM-score: 22.6)
    SepSecS; O-phosphoseryl-tRNA(Sec) selenium transferase, SepSecS (PF05889; HMM-score: 14.1)
    Pyridoxal_deC; Pyridoxal-dependent decarboxylase conserved domain (PF00282; HMM-score: 11.2)
    GHD (CL0369) DUF1945; Domain of unknown function (DUF1945) (PF09178; HMM-score: 8.5)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.176
    • Ymax_pos: 23
    • Cmax: 0.228
    • Cmax_pos: 51
    • Smax: 0.23
    • Smax_pos: 11
    • Smean: 0.152
    • D: 0.167
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MLILVLGGNVVISFENDYLEGAHEKVLKRLVDTNLVQASGYGFDQFTAQAIEKIKDTIDCPNATIRFLVGGTQTNQVVINSMLESYEGVISADTGHVAVHEGGAIEYSGHKVITIPSKEGKVSASDVETYMETFKSDFKKDHMVFPGMVYISHPTEYGTLYSKSELEELCKVCKQYQLPLFMDGARLGYGLMSDQSDMTIKDIAKYCDVFYIGGTKIGALCGEAIVFTKNNEPKQFTTRIKHHGALLAKGRLTGIQFLELFTDNLYFNISRHAIEMANKMKDGFKNKGYRLYFDSPTNQQFFILSNEKIAELEQKVKFAVWEKYDDQHRVVRFATSWATTEENLNKLLELI

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]