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NCBI: 06-JUL-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_1417 [new locus tag: NWMN_RS08000 ]
  • pan locus tag?: SAUPAN004063000
  • symbol: gnd
  • pan gene symbol?: gnd
  • synonym:
  • product: 6-phosphogluconate dehydrogenase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: NWMN_1417 [new locus tag: NWMN_RS08000 ]
  • symbol: gnd
  • product: 6-phosphogluconate dehydrogenase
  • replicon: chromosome
  • strand: -
  • coordinates: 1586478..1587884
  • length: 1407
  • essential: unknown other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGACACAACAAATTGGAGTTATTGGTTTAGCCGTTATGGGTAAAAACCTAGCTTGGAAT
    ATTGAATCACGCGGATATAGTGTATCTGTATTTAACCGCTCAAGTGAAAAAACTGATTTA
    ATGGTTGAAGAATCAAAAGGGAAAAATATTCATCCAACGTATTCATTGGAAGAATTTGTT
    AATTCTTTAGAAAAACCACGTAAAATTTTATTAATGGTTCAAGCAGGTAAAGCTACGGAC
    GCAACGATTGATAGTTTGTTACCTTTATTAGATGATGGAGATATTTTAATTGATGGTGGT
    AATACAAACTATCAAGATACTATCAGACGTAATAAAGCATTAGCACAAAGTGCAATCAAC
    TTTATTGGTATGGGCGTTTCTGGTGGTGAAATTGGAGCGTTAACTGGTCCGTCATTAATG
    CCGGGTGGACAAGAAGAAGCTTATAACAAAGTTGCAGATATTCTTGATGCAATTGCTGCA
    AAGGCAAAAGACGGTGCTTCATGTGTTACTTATATTGGACCTAATGGTGCTGGACACTAT
    GTGAAAATGGTACACAATGGTATCGAATATGCTGACATGCAATTAATTGCAGAAAGTTAT
    GCTATGATGAAAGAATTATTAGGCATGTCTCATGAAGACATTGCTCAAACGTTCAAAGAC
    TGGAATGCTGGTGAATTAGAAAGTTATTTAATTGAAATCACTGGTGATATTTTCATGAAA
    TTAGATGAAAATAAAGAAGCATTAGTTGAAAAAATTCTTGATACTGCAGGTCAAAAAGGT
    ACAGGAAAATGGACTTCAATTAACGCATTAGAATTAGGTATTCCATTAACAATCATTACT
    GAATCTGTATTTGCACGTTTTATCTCTTCAATCAAAGAAGAGCGCGTAAATGCTTCTAAA
    GAATTAAATGGTCCAAAAGCATCATTTGATGGTGACAAGAAAGACTTTTTAGAAAAAATT
    CGTAAAGCATTATATATGAGTAAAATTTGTTCTTATGCACAAGGTTTCGCGCAAATGCGT
    AAAGCAAGTGAAGATAATGAATGGAATTTAAAACTTGGTGATTTAGCTATGATTTGGAGA
    GAAGGTTGCATTATCCGTGCACAATTCTTACAAAAGATCAAAGATGCTTACGATAATAAT
    CCAGGATTACAAAACTTATTGCTAGATCCTTACTTTAAAAACATCGTTACTGAATATCAA
    GATGCGTTACGTGATGTTGTCGCAACTGGTGTTCAAAATGGTGTACCAACACCTGGTTTC
    TCATCAAGTATTAATTACTATGATAGTTACCGTGCTGCTGATTTACCTGCAAACTTAATT
    CAAGCACAACGTGACTACTTCGGTGCTCATACGTACGAAAGAAAAGATAAAGAAGGCGTA
    TTCCATACACAATGGATTGAAGAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1407

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: NWMN_1417 [new locus tag: NWMN_RS08000 ]
  • symbol: Gnd
  • description: 6-phosphogluconate dehydrogenase
  • length: 468
  • theoretical pI: 4.75082
  • theoretical MW: 51802.4
  • GRAVY: -0.300214

Function[edit source | edit]

  • reaction:
    EC 1.1.1.44?  ExPASy
    Phosphogluconate dehydrogenase (NADP+-dependent, decarboxylating)6-phospho-D-gluconate + NADP+ = D-ribulose 5-phosphate + CO2 + NADPH
  • TIGRFAM:
    MetabolismEnergy metabolismPentose phosphate pathway6-phosphogluconate dehydrogenase (decarboxylating) (TIGR00873; EC 1.1.1.44; HMM-score: 733.9)
    MetabolismEnergy metabolismPentose phosphate pathway6-phosphogluconate dehydrogenase (decarboxylating) (TIGR00872; EC 1.1.1.44; HMM-score: 229.9)
    2-hydroxy-3-oxopropionate reductase (TIGR01505; EC 1.1.1.60; HMM-score: 56)
    MetabolismEnergy metabolismAmino acids and amines3-hydroxyisobutyrate dehydrogenase (TIGR01692; EC 1.1.1.31; HMM-score: 49.7)
    MetabolismCentral intermediary metabolismPhosphorus compoundspolyphosphate:AMP phosphotransferase (TIGR03708; EC 2.7.4.-; HMM-score: 12.8)
  • TheSEED:  
    CarbohydratesCentral carbohydrate metabolismPentose phosphate pathway 6-phosphogluconate dehydrogenase, decarboxylating (EC 1.1.1.44) 
    MonosaccharidesD-gluconate and ketogluconates metabolism 6-phosphogluconate dehydrogenase, decarboxylating (EC 1.1.1.44) 
    One-carbon MetabolismFormaldehyde assimilation: Ribulose monophosphate pathway 6-phosphogluconate dehydrogenase, decarboxylating (EC 1.1.1.44) 
  • PFAM:
    6PGD_C (CL0106) 6PGD; 6-phosphogluconate dehydrogenase, C-terminal domain (PF00393; HMM-score: 446.4)
    NADP_Rossmann (CL0063) NAD_binding_2; NAD binding domain of 6-phosphogluconate dehydrogenase (PF03446; HMM-score: 173.9)
    Shikimate_DH; Shikimate / quinate 5-dehydrogenase (PF01488; HMM-score: 19)
    2-Hacid_dh_C; D-isomer specific 2-hydroxyacid dehydrogenase, NAD binding domain (PF02826; HMM-score: 16.4)
    F420_oxidored; NADP oxidoreductase coenzyme F420-dependent (PF03807; HMM-score: 14.2)
    3HCDH_N; 3-hydroxyacyl-CoA dehydrogenase, NAD binding domain (PF02737; HMM-score: 13.6)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:
    NWMN_0959(phdA)pyruvate dehydrogenase E1 component, alpha subunit  [1] (data from MRSA252)
    NWMN_2146(rpsC)30S ribosomal protein S3  [1] (data from MRSA252)
    NWMN_2119(rpsI)30S ribosomal protein S9  [1] (data from MRSA252)
    NWMN_1382DNA-binding protein HU  [1] (data from MRSA252)

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.157
    • Ymax_pos: 21
    • Cmax: 0.175
    • Cmax_pos: 21
    • Smax: 0.255
    • Smax_pos: 2
    • Smean: 0.157
    • D: 0.157
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MTQQIGVIGLAVMGKNLAWNIESRGYSVSVFNRSSEKTDLMVEESKGKNIHPTYSLEEFVNSLEKPRKILLMVQAGKATDATIDSLLPLLDDGDILIDGGNTNYQDTIRRNKALAQSAINFIGMGVSGGEIGALTGPSLMPGGQEEAYNKVADILDAIAAKAKDGASCVTYIGPNGAGHYVKMVHNGIEYADMQLIAESYAMMKELLGMSHEDIAQTFKDWNAGELESYLIEITGDIFMKLDENKEALVEKILDTAGQKGTGKWTSINALELGIPLTIITESVFARFISSIKEERVNASKELNGPKASFDGDKKDFLEKIRKALYMSKICSYAQGFAQMRKASEDNEWNLKLGDLAMIWREGCIIRAQFLQKIKDAYDNNPGLQNLLLDPYFKNIVTEYQDALRDVVATGVQNGVPTPGFSSSINYYDSYRAADLPANLIQAQRDYFGAHTYERKDKEGVFHTQWIEE

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. 1.0 1.1 1.2 1.3 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J. Proteome Res.: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)

Relevant publications[edit source | edit]