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NCBI: 06-JUL-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_1616 [new locus tag: NWMN_RS09065 ]
  • pan locus tag?: SAUPAN004378000
  • symbol: NWMN_1616
  • pan gene symbol?:
  • synonym:
  • product: aminotransferase, class V

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: NWMN_1616 [new locus tag: NWMN_RS09065 ]
  • symbol: NWMN_1616
  • product: aminotransferase, class V
  • replicon: chromosome
  • strand: +
  • coordinates: 1793578..1794738
  • length: 1161
  • essential: unknown other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGTATTATCATCAACCGTTGTTATTAACACCTGGCCCAACCCCTGTACCTGATGCCATT
    ATGAGAGAAATTCAAGCACCTATGGTTGGTCATCGTTCTAAAGATTTTGAAGACATCGCA
    CAACAAGCATTTCAAGGTCTAAAGCCAATATTTGGGAGTCAAAATGATGTACTTATTTTA
    ACATCTAGCGGTACAAGCGTCTTGGAGGCTAGTATGTTGAACATTGTAAACCCTGAAGAT
    CACTTCGTTGTCATTGTTTCAGGTGCCTTTGGTAACCGATTTAAACAAATTGCACAAACT
    TATTACAAAAATGTGCATATTTATGACGTAACATGGGGAGAAGCTGTAGATGTCAAAGAT
    TTCATCAATTTCCTTTCAACTTTAAATGTTGAAGTTAAAGCAGTATTTAGTCAATATTGC
    GAAACATCTACGACAGTGCTACACCCTATTCACGAGTTAGGAAATGCCATTAATCAATTT
    AATAGTAATATTTATTTTGTAGTTGACGGCGTAAGTTGCATTGGTGCTGTTGATGTTGAC
    ATTAACAAAGATAAAATTGATGTACTTGTTTCTGGTAGTCAAAAAGCAATTATGTTACCT
    CCAGGATTAGCTTTTGTAGCTTATAGCCACCGTGCAAAAGAACATTTCAAAGAAGTAACT
    ACGCCAAAATTTTATCTAGACTTAAATAAATACATTTCGTCACAAGCTGACAATTCTACA
    CCGTTCACACCAAATGTGTCTTTATTTAGAGGTGTAAATGCATACGTTGAAACCGTAAAA
    GCAGAAGGTTTCAATCACGTAATAGCACGACACTATGCAATTAGAAATGCATTAAGAAGC
    GCCTTAAAAGCATTAGATTTAACTTTATTAGTCAATGATAAAGATGCATCTCCAACGGTT
    ACAGCATTCAAACCTAATACAAATGATGAAGTGAAAATAATCAAAGATGAACTTAAAAAT
    CGCTTTAAAATAACAATTGCTGGTGGTCAAGGCCATCTTAAAGGTCAAATTTTAAGAATT
    GGTCATATGGGGAAAATTAGTCCTTTCGATATTTTATCGGTAGTATCTGCTTTAGAAATT
    ATTTTAACTGAACACCGTAAAGTTAACTATATCGGTAAAGGTATATCAAAATATATGGAG
    GTTATTCATGAAGCAATTTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1161

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: NWMN_1616 [new locus tag: NWMN_RS09065 ]
  • symbol: NWMN_1616
  • description: aminotransferase, class V
  • length: 386
  • theoretical pI: 7.24118
  • theoretical MW: 42849.9
  • GRAVY: -0.0124352

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    2-aminoethylphosphonate aminotransferase (TIGR03301; EC 2.6.1.-; HMM-score: 171.9)
    MetabolismCentral intermediary metabolismPhosphorus compounds2-aminoethylphosphonate--pyruvate transaminase (TIGR02326; EC 2.6.1.37; HMM-score: 123.9)
    MetabolismAmino acid biosynthesisSerine familyphosphoserine transaminase (TIGR01364; EC 2.6.1.52; HMM-score: 37.7)
    cysteine desulfurase NifS (TIGR03402; EC 2.8.1.7; HMM-score: 32.3)
    Unknown functionEnzymes of unknown specificitycysteine desulfurase family protein (TIGR01977; HMM-score: 28)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersGlutathione and analogsergothioneine biosynthesis PLP-dependent enzyme EgtE (TIGR04343; EC 4.4.-.-; HMM-score: 25.5)
    Genetic information processingDNA metabolismRestriction/modificationcysteine desulfurase DndA (TIGR03235; EC 2.8.1.7; HMM-score: 22)
    cysteine desulfurase, NifS family (TIGR03403; EC 2.8.1.7; HMM-score: 17.3)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersOthercysteine desulfurase, SufS family (TIGR01979; HMM-score: 16.7)
    Genetic information processingProtein synthesistRNA and rRNA base modificationcysteine desulfurase IscS (TIGR02006; EC 2.8.1.7; HMM-score: 13.6)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersOthercysteine desulfurase IscS (TIGR02006; EC 2.8.1.7; HMM-score: 13.6)
  • TheSEED:  
    Amino Acids and DerivativesAlanine, serine, and glycineSerine Biosynthesis Phosphoserine aminotransferase, putative (EC 2.6.1.52) 
  • PFAM:
    PLP_aminotran (CL0061) Aminotran_5; Aminotransferase class-V (PF00266; HMM-score: 137.5)
    Cys_Met_Meta_PP; Cys/Met metabolism PLP-dependent enzyme (PF01053; HMM-score: 13.5)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors: pyridoxal 5'-phosphate
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.67
    • Cytoplasmic Membrane Score: 0.01
    • Cellwall Score: 0.15
    • Extracellular Score: 0.17
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.124
    • Ymax_pos: 11
    • Cmax: 0.116
    • Cmax_pos: 36
    • Smax: 0.23
    • Smax_pos: 1
    • Smean: 0.136
    • D: 0.129
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MYYHQPLLLTPGPTPVPDAIMREIQAPMVGHRSKDFEDIAQQAFQGLKPIFGSQNDVLILTSSGTSVLEASMLNIVNPEDHFVVIVSGAFGNRFKQIAQTYYKNVHIYDVTWGEAVDVKDFINFLSTLNVEVKAVFSQYCETSTTVLHPIHELGNAINQFNSNIYFVVDGVSCIGAVDVDINKDKIDVLVSGSQKAIMLPPGLAFVAYSHRAKEHFKEVTTPKFYLDLNKYISSQADNSTPFTPNVSLFRGVNAYVETVKAEGFNHVIARHYAIRNALRSALKALDLTLLVNDKDASPTVTAFKPNTNDEVKIIKDELKNRFKITIAGGQGHLKGQILRIGHMGKISPFDILSVVSALEIILTEHRKVNYIGKGISKYMEVIHEAI

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator: CodY
    CodY (TF) important in Amino acid metabolism  RegPrecise

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]