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NCBI: 06-JUL-2013

Summary[edit | edit source]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_1625 [new locus tag: NWMN_RS09125 ]
  • pan locus tag?: SAUPAN004396000
  • symbol: fhs
  • pan gene symbol?: fhs
  • synonym:
  • product: formate--tetrahydrofolate ligase

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: NWMN_1625 [new locus tag: NWMN_RS09125 ]
  • symbol: fhs
  • product: formate--tetrahydrofolate ligase
  • replicon: chromosome
  • strand: -
  • coordinates: 1807240..1808907
  • length: 1668
  • essential: unknown other strains

Accession numbers[edit | edit source]

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    TTGACTCATTTATCAGATTTAGATATTGCGAATCAATCAACACTACAACCAATTAAGGAT
    ATTGCTGCATCAGTAGGTATTTCAGAGGATGCATTAGAACCTTATGGTCATTACAAAGCT
    AAAATCGACATTAATAAAATTACGCCAAGAGAAAACAAAGGGAAAGTTGTTTTAGTAACT
    GCGATGAGCCCAACACCAGCTGGTGAAGGTAAATCAACGGTTACAGTTGGTTTAGCTGAT
    GCATTCCATGAGTTAAATAAAAACGTTATGGTTGCATTAAGAGAGCCTGCTTTAGGACCA
    ACATTTGGTATCAAAGGTGGTGCGACTGGTGGTGGTTATGCGCAAGTCTTACCTATGGAA
    GATATCAACTTACATTTCAACGGAGATTTCCATGCGATTACAACTGCAAATAATGCATTG
    TCTGCGTTTATCGATAATCATATTCACCAAGGTAACGAATTAGGAATCGATCAAAGACGT
    ATTGAGTGGAAACGTGTATTAGATATGAATGATCGTGCACTTAGACATGTAAACGTTGGG
    TTAGGTGGACCTACAAATGGTGTACCACGTGAAGATGGCTTTAATATTACAGTAGCGTCT
    GAAATTATGGCGATTTTATGTTTAAGTAGAAGTATTAAAGACTTAAAAGATAAAATTAGT
    CGTATTACTATTGGTTACACTAGAGATCGCAAGCCAGTTACAGTTGCAGATTTAAAAGTG
    GAAGGTGCACTTGCAATGATTTTAAAAGATGCAATAAAACCAAACTTAGTACAATCAATT
    GAAGGGACACCTGCATTAGTTCATGGTGGACCATTTGCGAATATCGCACACGGTTGTAAC
    TCAATTTTAGCAACTGAAACAGCACGTGATTTAGCTGATATCGTTGTAACGGAAGCTGGA
    TTTGGTTCAGACTTAGGCGCTGAAAAATTCATGGACATTAAAGTGCGTGAAGCAGGATTT
    GATCCGGCAGCTGTCGTTGTTGTTGCGACAATTCGTGCGTTAAAAATGCATGGTGGTGTA
    GCGAAAGATAATTTAAAAGAAGAAAATGTAGAAGCAGTAAAAGCAGGAATTGTTAATTTA
    GAGCGTCATGTTAATAATATTAAAAAATTCGGTGTAGAACCGGTTGTTGCAATTAATGCA
    TTTATACATGATACCGATGCAGAAGTAGAATATGTAAAATCTTGGGCTAAAGAAAATAAC
    GTACGAATTGCCTTAACTGAAGTTTGGAAAAAAGGTGGTAAAGGTGGCGTTGACTTAGCA
    AATGAAGTATTAGAAGTCATTGATCAACCTAATTCATTTAAACCTTTATATGAATTAGAA
    TTACCATTAGAGCAAAAGATTGAAAAGATTGTGACTGAAATCTATGGCGGTTCAAAAGTA
    ACGTTTAGCAGTAAAGCGCAAAAACAATTAAAACAATTTAAAGAAAATGGTTGGGATAAT
    TACCCAGTATGTATGGCGAAAACACAATATTCATTCTCAGATGATCAAACGTTGTTAGGT
    GCACCATCAGGATTTGAAATTACAATTCGTGAATTAGAAGCGAAAACAGGTGCAGGATTT
    ATCGTAGCGTTGACAGGTGCAATCATGACTATGCCTGGTTTACCTAAAAAACCAGCAGCA
    TTAAACATGGATGTTACTGATGATGGTCATGCAATTGGGTTATTCTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1668

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: NWMN_1625 [new locus tag: NWMN_RS09125 ]
  • symbol: Fhs
  • description: formate--tetrahydrofolate ligase
  • length: 555
  • theoretical pI: 6.04634
  • theoretical MW: 59883.2
  • GRAVY: -0.111532

Function[edit | edit source]

  • reaction:
    EC 6.3.4.3?  ExPASy
    Formate--tetrahydrofolate ligase ATP + formate + tetrahydrofolate = ADP + phosphate + 10-formyltetrahydrofolate
  • TIGRFAM:
  • TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
  • PFAM:
    P-loop_NTPase (CL0023) FTHFS; Formate--tetrahydrofolate ligase (PF01268; HMM-score: 881.8)
    and 2 more
    POTRA (CL0191) POTRA_TamA_1; POTRA domain TamA domain 1 (PF17243; HMM-score: 15.9)
    P-loop_NTPase (CL0023) CbiA; CobQ/CobB/MinD/ParA nucleotide binding domain (PF01656; HMM-score: 14.5)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.005497
    • TAT(Tat/SPI): 0.001617
    • LIPO(Sec/SPII): 0.001074
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MTHLSDLDIANQSTLQPIKDIAASVGISEDALEPYGHYKAKIDINKITPRENKGKVVLVTAMSPTPAGEGKSTVTVGLADAFHELNKNVMVALREPALGPTFGIKGGATGGGYAQVLPMEDINLHFNGDFHAITTANNALSAFIDNHIHQGNELGIDQRRIEWKRVLDMNDRALRHVNVGLGGPTNGVPREDGFNITVASEIMAILCLSRSIKDLKDKISRITIGYTRDRKPVTVADLKVEGALAMILKDAIKPNLVQSIEGTPALVHGGPFANIAHGCNSILATETARDLADIVVTEAGFGSDLGAEKFMDIKVREAGFDPAAVVVVATIRALKMHGGVAKDNLKEENVEAVKAGIVNLERHVNNIKKFGVEPVVAINAFIHDTDAEVEYVKSWAKENNVRIALTEVWKKGGKGGVDLANEVLEVIDQPNSFKPLYELELPLEQKIEKIVTEIYGGSKVTFSSKAQKQLKQFKENGWDNYPVCMAKTQYSFSDDQTLLGAPSGFEITIRELEAKTGAGFIVALTGAIMTMPGLPKKPAALNMDVTDDGHAIGLF

Experimental data[edit | edit source]

  • experimentally validated: data available for NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:
    NWMN_0961(pdhC)branched-chain alpha-keto acid dehydrogenase subunit E2  [1] (data from MRSA252)
    NWMN_0959(phdA)pyruvate dehydrogenase E1 component, alpha subunit  [1] (data from MRSA252)
    NWMN_0510(tufA)elongation factor Tu  [1] (data from MRSA252)

Expression & Regulation[edit | edit source]

Operon[edit | edit source]

Regulation[edit | edit source]

  • regulator: CcpA regulon
    CcpA(TF)important in Carbon catabolism; RegPrecise 

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

  1. 1.0 1.1 1.2 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J Proteome Res: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)

Relevant publications[edit | edit source]