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NCBI: 03-AUG-2016

Summary[edit source | edit]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_01845
  • pan locus tag?: SAUPAN004396000
  • symbol: SAOUHSC_01845
  • pan gene symbol?: fhs
  • synonym:
  • product: formate--tetrahydrofolate ligase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SAOUHSC_01845
  • symbol: SAOUHSC_01845
  • product: formate--tetrahydrofolate ligase
  • replicon: chromosome
  • strand: -
  • coordinates: 1750440..1752107
  • length: 1668
  • essential: no DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    TTGACTCATTTATCAGATTTAGATATTGCGAATCAATCAACACTACAACCAATTAAGGAT
    ATTGCTGCATCAGTAGGTATTTCAGAGGATGCATTAGAACCTTATGGTCATTACAAAGCT
    AAAATCGACATTAATAAAATTACGCCAAGAGAAAACAAAGGGAAAGTTGTTTTAGTAACT
    GCGATGAGCCCAACACCAGCTGGTGAAGGTAAATCAACGGTTACAGTTGGTTTAGCTGAT
    GCATTCCATGAGTTAAATAAAAACGTTATGGTTGCATTAAGAGAGCCTGCTTTAGGACCA
    ACATTTGGTATCAAAGGTGGTGCGACTGGTGGTGGTTATGCGCAAGTCTTACCTATGGAA
    GATATCAACTTACATTTCAACGGAGATTTCCATGCGATTACAACTGCAAATAATGCATTG
    TCTGCGTTTATCGATAATCATATTCACCAAGGTAACGAATTAGGAATCGATCAAAGACGT
    ATTGAGTGGAAACGTGTATTAGATATGAATGATCGTGCACTTAGACATGTAAACGTTGGG
    TTAGGTGGACCTACAAATGGTGTACCACGTGAAGATGGCTTTAATATTACAGTAGCGTCT
    GAAATTATGGCGATTTTATGTTTAAGTAGAAGTATTAAAGACTTAAAAGATAAAATTAGT
    CGTATTACTATTGGTTACACTAGAGATCGCAAGCCAGTTACAGTTGCAGATTTAAAAGTG
    GAAGGTGCACTTGCAATGATTTTAAAAGATGCAATAAAACCAAACTTAGTACAATCAATT
    GAAGGGACACCTGCATTAGTTCATGGTGGACCATTTGCGAATATCGCACACGGTTGTAAC
    TCAATTTTAGCAACTGAAACAGCACGTGATTTAGCTGATATCGTTGTAACGGAAGCTGGA
    TTTGGTTCAGACTTAGGCGCTGAAAAATTCATGGACATTAAAGCGCGTGAAGCAGGATTT
    GATCCGGCAGCTGTCGTTGTTGTTGCGACAATTCGTGCGTTAAAAATGCATGGTGGTGTA
    GCGAAAGATAATTTAAAAGAAGAAAATGTAGAAGCAGTAAAAGCAGGAATTGTTAATTTA
    GAGCGTCATGTTAATAATATTAAAAAATTCGGTGTAGAACCGGTTGTTGCAATTAATGCA
    TTTATACATGATACCGATGCAGAAGTAGAATATGTAAAATCTTGGGCTAAAGAAAATAAC
    GTACGAATTGCCTTAACTGAAGTTTGGGAAAAAGGTGGTAAAGGTGGCGTTGACTTAGCA
    AATGAAGTATTAGAAGTCATTGATCAACCTAATTCATTTAAACCTTTATATGAATTAGAA
    TTACCATTAGAGCAAAAGATTGAAAAGATTGTGACTGAAATCTATGGCGGTTCAAAAGTA
    ACGTTTAGCAGTAAAGCGCAAAAACAATTAAAACAATTTAAAGAAAATGGTTGGGATAAT
    TACCCAGTATGTATGGCGAAAACACAATATTCATTCTCAGATGATCAAACGTTGTTAGGT
    GCACCATCAGGATTTGAAATTACAATTCGTGAATTAGAAGCGAAAACAGGTGCAGGATTT
    ATCGTAGCGTTGACAGGTGCAATCATGACTATGCCTGGTTTACCTAAAAAACCAGCAGCA
    TTAAACATGGATGTTACTGATGATGGTCATGCAATTGGGTTATTCTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1668

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SAOUHSC_01845
  • symbol: SAOUHSC_01845
  • description: formate--tetrahydrofolate ligase
  • length: 555
  • theoretical pI: 5.77686
  • theoretical MW: 59856.1
  • GRAVY: -0.115135

Function[edit source | edit]

  • reaction:
    EC 6.3.4.3?  ExPASy
    Formate--tetrahydrofolate ligaseATP + formate + tetrahydrofolate = ADP + phosphate + 10-formyltetrahydrofolate
  • TIGRFAM:
  • TheSEED:  
    CarbohydratesOne-carbon MetabolismOne-carbon metabolism by tetrahydropterines Formate--tetrahydrofolate ligase (EC 6.3.4.3) 
    One-carbon MetabolismSerine-glyoxylate cycle Formate--tetrahydrofolate ligase (EC 6.3.4.3) 
    Cofactors, Vitamins, Prosthetic Groups, PigmentsFolate and pterinesFolate Biosynthesis Formate--tetrahydrofolate ligase (EC 6.3.4.3) 
    MiscellaneousMiscellaneous - no subcategoryEC 6.3.4.- Ligases that form carbon-nitrogen bonds Formate--tetrahydrofolate ligase (EC 6.3.4.3) 
  • PFAM:
    P-loop_NTPase (CL0023) FTHFS; Formate--tetrahydrofolate ligase (PF01268; HMM-score: 882.5)
    POTRA (CL0191) POTRA_TamA_1; POTRA domain TamA domain 1 (PF17243; HMM-score: 16)
    P-loop_NTPase (CL0023) CbiA; CobQ/CobB/MinD/ParA nucleotide binding domain (PF01656; HMM-score: 14.5)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:
    SAOUHSC_00530elongation factor Tu  [1] (data from MRSA252)
    SAOUHSC_01040pyruvate dehydrogenase complex, E1 component subunit alpha  [1] (data from MRSA252)
    SAOUHSC_01042branched-chain alpha-keto acid dehydrogenase subunit E2  [1] (data from MRSA252)

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.184
    • Ymax_pos: 23
    • Cmax: 0.221
    • Cmax_pos: 41
    • Smax: 0.243
    • Smax_pos: 20
    • Smean: 0.125
    • D: 0.161
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MTHLSDLDIANQSTLQPIKDIAASVGISEDALEPYGHYKAKIDINKITPRENKGKVVLVTAMSPTPAGEGKSTVTVGLADAFHELNKNVMVALREPALGPTFGIKGGATGGGYAQVLPMEDINLHFNGDFHAITTANNALSAFIDNHIHQGNELGIDQRRIEWKRVLDMNDRALRHVNVGLGGPTNGVPREDGFNITVASEIMAILCLSRSIKDLKDKISRITIGYTRDRKPVTVADLKVEGALAMILKDAIKPNLVQSIEGTPALVHGGPFANIAHGCNSILATETARDLADIVVTEAGFGSDLGAEKFMDIKAREAGFDPAAVVVVATIRALKMHGGVAKDNLKEENVEAVKAGIVNLERHVNNIKKFGVEPVVAINAFIHDTDAEVEYVKSWAKENNVRIALTEVWEKGGKGGVDLANEVLEVIDQPNSFKPLYELELPLEQKIEKIVTEIYGGSKVTFSSKAQKQLKQFKENGWDNYPVCMAKTQYSFSDDQTLLGAPSGFEITIRELEAKTGAGFIVALTGAIMTMPGLPKKPAALNMDVTDDGHAIGLF

Experimental data[edit source | edit]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. 1.0 1.1 1.2 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J. Proteome Res.: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)
  2. Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  3. Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-61
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  4. Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet.: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

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