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NCBI: 03-AUG-2016

Summary[edit source | edit]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_01850
  • pan locus tag?: SAUPAN004400000
  • symbol: SAOUHSC_01850
  • pan gene symbol?: ccpA
  • synonym:
  • product: catabolite control protein A

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SAOUHSC_01850
  • symbol: SAOUHSC_01850
  • product: catabolite control protein A
  • replicon: chromosome
  • strand: -
  • coordinates: 1756346..1757335
  • length: 990
  • essential: no DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGACAGTTACTATATATGATGTAGCAAGAGAAGCGCGTGTCTCTATGGCCACAGTGTCG
    CGTGTTGTTAATGGGAACCAAAATGTTAAAGCAGAAACTAAAAATAAAGTTAACGAAGTC
    ATTAAGCGTTTGAATTATCGTCCAAATGCTGTTGCTAGAGGTTTAGCTAGTAAAAAGACA
    ACAACAGTAGGTGTGATCATTCCAGATATATCTAATATCTATTATTCACAACTTGCTCGT
    GGACTTGAAGATATTGCAACAATGTATAAATATCACTCAATTATTTCAAATTCAGATAAC
    GATCCTGAAAAGGAAAAAGAAATTTTTAATAACTTATTAAGTAAACAGGTTGATGGTATT
    ATTTTCCTTGGTGGTACAATTACTGAAGAAATGAAAGAATTGATAAATCAATCATCTGTA
    CCTGTAGTAGTATCAGGAACAAATGGTAAGGATGCACATATAGCATCAGTTAATATTGAT
    TTTACTGAAGCTGCGAAAGAAATTACGGGAGAATTAATTGAAAAAGGCGCTAAATCATTT
    GCTTTAGTAGGTGGAGAACATTCTAAAAAAGCTCAAGAAGATGTTTTAGAAGGTTTAACT
    GAAGTGTTAAATAAAAATGGCCTTCAATTAGGTGATACATTGAATTGTTCTGGTGCTGAA
    AGTTATAAAGAAGGCGTAAAAGCTTTTGCCAAAATGAAAGGCAATTTGCCAGATGCCATT
    TTATGTATCAGCGACGAAGAAGCAATTGGTATTATGCATAGTGCAATGGATGCTGGTATT
    AAAGTTCCAGAGGAATTACAAATTATTAGTTTCAATAATACACGATTAGTTGAGATGGTT
    AGACCACAACTTTCTAGTGTTATTCAACCATTATATGATATCGGTGCAGTAGGGATGCGC
    TTATTAACAAAATATATGAACGATGAAAAGATAGAAGAACCAAATGTAGTTTTACCTCAC
    AGAATTGAATACCGAGGAACTACAAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    990

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SAOUHSC_01850
  • symbol: SAOUHSC_01850
  • description: catabolite control protein A
  • length: 329
  • theoretical pI: 5.50763
  • theoretical MW: 36060
  • GRAVY: -0.229787

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    Signal transductionRegulatory functionsDNA interactionscatabolite control protein A (TIGR01481; HMM-score: 570.3)
    Signal transductionRegulatory functionsDNA interactionsD-fructose-responsive transcription factor (TIGR02417; HMM-score: 159)
    Signal transductionRegulatory functionsDNA interactionstrehalose operon repressor (TIGR02405; HMM-score: 107.1)
    Genetic information processingMobile and extrachromosomal element functionsOtheraddiction module antidote protein, HigA family (TIGR02607; HMM-score: 18.6)
    Signal transductionRegulatory functionsDNA interactionsaddiction module antidote protein, HigA family (TIGR02607; HMM-score: 18.6)
    Signal transductionRegulatory functionsProtein interactionsaddiction module antidote protein, HigA family (TIGR02607; HMM-score: 18.6)
  • TheSEED:  
    Regulation and Cell signalingRegulation and Cell signaling - no subcategoryHPr catabolite repression system Catabolite control protein A 
  • PFAM:
    Periplas_BP (CL0144) Peripla_BP_3; Periplasmic binding protein-like domain (PF13377; HMM-score: 105.4)
    Peripla_BP_4; Periplasmic binding protein domain (PF13407; HMM-score: 74.7)
    Peripla_BP_1; Periplasmic binding proteins and sugar binding domain of LacI family (PF00532; HMM-score: 71.1)
    HTH (CL0123) LacI; Bacterial regulatory proteins, lacI family (PF00356; HMM-score: 67.1)
    no clan definedATP-synt_F; ATP synthase (F/14-kDa) subunit (PF01990; HMM-score: 16.2)
    4Fe-4S (CL0344) Fer4_12; 4Fe-4S single cluster domain (PF13353; HMM-score: 14.6)
    MurF-HprK_N (CL0365) Hpr_kinase_N; HPr Serine kinase N terminus (PF02603; HMM-score: 14.5)
    no clan definedDUF327; Protein of unknown function (DUF327) (PF03885; HMM-score: 13.9)
    HTH (CL0123) HTH_3; Helix-turn-helix (PF01381; HMM-score: 12.9)
    HTH_31; Helix-turn-helix domain (PF13560; HMM-score: 12.7)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: Signal peptide length 32 aa
    • Ymax: 0.542
    • Ymax_pos: 32
    • Cmax: 0.674
    • Cmax_pos: 32
    • Smax: 0.69
    • Smax_pos: 29
    • Smean: 0.354
    • D: 0.469
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MTVTIYDVAREARVSMATVSRVVNGNQNVKAETKNKVNEVIKRLNYRPNAVARGLASKKTTTVGVIIPDISNIYYSQLARGLEDIATMYKYHSIISNSDNDPEKEKEIFNNLLSKQVDGIIFLGGTITEEMKELINQSSVPVVVSGTNGKDAHIASVNIDFTEAAKEITGELIEKGAKSFALVGGEHSKKAQEDVLEGLTEVLNKNGLQLGDTLNCSGAESYKEGVKAFAKMKGNLPDAILCISDEEAIGIMHSAMDAGIKVPEELQIISFNNTRLVEMVRPQLSSVIQPLYDIGAVGMRLLTKYMNDEKIEEPNVVLPHRIEYRGTTK

Experimental data[edit source | edit]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator: SAOUHSC_01850 (CcpA*)
    CcpA* (TF) important in Carbon catabolism  RegPrecise

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  2. Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-61
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  3. Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet.: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

Relevant publications[edit source | edit]