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NCBI: 03-AUG-2016

Summary[edit source | edit]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_00195
  • pan locus tag?: SAUPAN001092000
  • symbol: SAOUHSC_00195
  • pan gene symbol?: fadA
  • synonym:
  • product: acetyl-CoA acetyltransferase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SAOUHSC_00195
  • symbol: SAOUHSC_00195
  • product: acetyl-CoA acetyltransferase
  • replicon: chromosome
  • strand: -
  • coordinates: 214246..215430
  • length: 1185
  • essential: no DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGCAAGAAGCATACATTGTAGCTTATGGGCGTTCAGCCGCAGCGAAAGCAAAGCAAGGC
    GCATTATTCCACGAAAGACCTGATGATGTCGCAGCCAAAGTATTACAAGGCGTATTGAAA
    CGTATTGACGGAAAATTCAATAAGAATATGATTGAAGATGTCATTGTTGGTACGGCTTTT
    CCAGAAGGATTACAAGGCCAAAACATTGCACGAACGATTGCATTGCGTGCGGGATTATCT
    GACACGGTACCGGGTCAAACAGTGAATCGCTACTGCTCATCAGGATTACAAACCATCGCG
    ATTGCAGCCAATCAAATTATGGCTGGTCAAGGAGATATACTTGTAGCTGGTGGCGTTGAA
    TTGATGAGTGCCGTACCAATGGGTGGCAACGAGCCCACAAACAATCCAACCTTACAATAT
    GATGATATAGGTGCGTCATATCCTATGGGTTTAACTGCTGAAAATGTAGCATCCCAATTT
    GACGTATCACGCGAAGATCAAGATGCTTATGCTGTCAGAAGTCATCAACGTGCCTATGAC
    GCACAACGTGATGGTCGGTTCAAAGATGAAATTATTCCAATACAAGTAAACTCAGTTGAA
    TATACAAACGCAGGACCAAAAGTACACACAAATATCTTTGACCAAGATGAATTTATACGC
    CCTGACACCACGATGGAGGCATTAGCCAAATTACGTACAGTATTTAAAGCTGACGGCACT
    ATGACTGCAGGAACATCTGCCCCACTTTCTGATGGTGCAGGATTTGTAGTTTTAATGTCT
    GGAGATAAAGTGAAAGAACTCGGCGTGACACCTATTGCACGATTCGTTGGTTTTAAGGCA
    GTAGGCGTTGACCCGAAAATTATGGGTATTGGGCCTGCATATGCGATTCCTGAAGTATTG
    TCACTCAGCAATCTATCTGTTGAAGACATTGATTTGATCGAATTGAACGAAGCATTTGCT
    TCTCAAACGATTGCATCTATTAAAGAAGTAGGTCTAGATATATCACGTACGAATGTGAAT
    GGTGGCGCTATTGCTTTAGGTCATCCATTAGGTGCTACAGGCGCAATGTTAACCGCGCGT
    TTACTTAATGAAATGGGTAGACGTCCCGATAGCCGTTACGGCATGGTTACGATGTGTATT
    GGTGTCGGCATGGGTGCAGCTGCTATATTTGAATATGTGCGTTAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1185

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SAOUHSC_00195
  • symbol: SAOUHSC_00195
  • description: acetyl-CoA acetyltransferase
  • length: 394
  • theoretical pI: 4.8083
  • theoretical MW: 41836.4
  • GRAVY: -0.019797

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    MetabolismFatty acid and phospholipid metabolismOtheracetyl-CoA C-acyltransferase (TIGR01930; EC 2.3.1.16; HMM-score: 454)
    MetabolismFatty acid and phospholipid metabolismDegradationacetyl-CoA C-acyltransferase FadA (TIGR02445; EC 2.3.1.16; HMM-score: 346.5)
    3-oxoadipyl-CoA thiolase (TIGR02430; EC 2.3.1.174; HMM-score: 341.9)
    MetabolismFatty acid and phospholipid metabolismDegradationacetyl-CoA C-acyltransferase FadI (TIGR02446; EC 2.3.1.16; HMM-score: 195.7)
    MetabolismFatty acid and phospholipid metabolismBiosynthesisbeta-ketoacyl-acyl-carrier-protein synthase II (TIGR03150; EC 2.3.1.179; HMM-score: 32.5)
  • TheSEED:  
    Amino Acids and DerivativesBranched-chain amino acidsIsoleucine degradation 3-ketoacyl-CoA thiolase (EC 2.3.1.16) 
    CarbohydratesOne-carbon MetabolismSerine-glyoxylate cycle 3-ketoacyl-CoA thiolase (EC 2.3.1.16) 
    Cofactors, Vitamins, Prosthetic Groups, PigmentsBiotinBiotin biosynthesis 3-ketoacyl-CoA thiolase (EC 2.3.1.16) 
    Fatty Acids, Lipids, and IsoprenoidsFatty Acids, Lipids, and Isoprenoids - no subcategoryPolyhydroxybutyrate metabolism 3-ketoacyl-CoA thiolase (EC 2.3.1.16) 
  • PFAM:
    Thiolase (CL0046) Thiolase_N; Thiolase, N-terminal domain (PF00108; HMM-score: 238.6)
    Thiolase_C; Thiolase, C-terminal domain (PF02803; HMM-score: 150.6)
    ketoacyl-synt; Beta-ketoacyl synthase, N-terminal domain (PF00109; HMM-score: 23.4)
    P-loop_NTPase (CL0023) Podovirus_Gp16; Podovirus DNA encapsidation protein (Gp16) (PF05894; HMM-score: 12)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:
    SAOUHSC_02511(rplD)50S ribosomal protein L4  [1] (data from MRSA252)

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helix: 1
  • LocateP: Intracellular /TMH start AFTER 60
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: Possibly Sec-
    • Intracellular Possibility: 0.17
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.112
    • Ymax_pos: 33
    • Cmax: 0.116
    • Cmax_pos: 33
    • Smax: 0.161
    • Smax_pos: 6
    • Smean: 0.097
    • D: 0.106
  • predicted transmembrane helices (TMHMM): 1

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MQEAYIVAYGRSAAAKAKQGALFHERPDDVAAKVLQGVLKRIDGKFNKNMIEDVIVGTAFPEGLQGQNIARTIALRAGLSDTVPGQTVNRYCSSGLQTIAIAANQIMAGQGDILVAGGVELMSAVPMGGNEPTNNPTLQYDDIGASYPMGLTAENVASQFDVSREDQDAYAVRSHQRAYDAQRDGRFKDEIIPIQVNSVEYTNAGPKVHTNIFDQDEFIRPDTTMEALAKLRTVFKADGTMTAGTSAPLSDGAGFVVLMSGDKVKELGVTPIARFVGFKAVGVDPKIMGIGPAYAIPEVLSLSNLSVEDIDLIELNEAFASQTIASIKEVGLDISRTNVNGGAIALGHPLGATGAMLTARLLNEMGRRPDSRYGMVTMCIGVGMGAAAIFEYVR

Experimental data[edit source | edit]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J. Proteome Res.: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)
  2. Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  3. Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-3661
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  4. Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet.: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

Relevant publications[edit source | edit]