From AureoWiki
Jump to: navigation, search
NCBI: 03-AUG-2016

Summary[edit source | edit]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_00155
  • pan locus tag?: SAUPAN001032000
  • symbol: SAOUHSC_00155
  • pan gene symbol?: glcA
  • synonym:
  • product: PTS system glucose-specific protein

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SAOUHSC_00155
  • symbol: SAOUHSC_00155
  • product: PTS system glucose-specific protein
  • replicon: chromosome
  • strand: -
  • coordinates: 166663..168660
  • length: 1998
  • essential: no DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGTTACCTGTTGCGATTTTACCAGCAGCTGGTCTGTTATTAGCTATCGGTACAGCTATG
    CAAGGTGAATCATTACAACACTACTTGCCGTTTATACAAAATGGTGGCGTACAAACTGTC
    GCTAAATTAATGACAGGTGCTGGTGGTATCATTTTTGATAACTTGCCTATGATTTTCGCA
    TTAGGTGTCGCAATCGGATTAGCTGGCGGTGATGGCGTAGCAGCTATCGCAGCATTCGTC
    GGTTACATAATCATGAACAAAACAATGGGCGACTTTTTACAAGTTACACCTAAGAATATT
    GGTGATCCAGCGAGTGGTTACGCTAGCATTTTAGGTATCCCAACATTACAAACAGGTGTG
    TTCGGCGGTATTATAATCGGGGCCCTGGCAGCTTGGTGTTATAACAAGTTCTATAACATT
    AACTTACCATCTTATTTAGGTTTCTTCGCTGGTAAGCGTTTCGTACCTATTATGATGGCT
    ACAACATCATTTATTTTAGCATTCCCAATGGCATTAATTTGGCCAACGATTCAATCAGGA
    TTAAATGCATTCAGTACAGGATTATTAGATTCAAATACTGGTGTTGCCGTATTCTTATTT
    GGTTTCATCAAGCGTTTATTAATTCCATTCGGTCTACATCACATTTTCCACGCACCGTTC
    TGGTTCGAGTTTGGTTCATGGAAAAATGCAGCTGGTGAAATTATTCACGGTGACCAACGT
    ATCTTTATCGAACAAATTCGTGAAGGCGCACATTTGACAGCTGGTAAATTCATGCAAGGT
    GAATTCCCTGTTATGATGTTCGGTTTACCTGCAGCAGCTTTAGCAATTTATCACACAGCT
    AAACCTGAAAATAAGAAAGTAGTAGCAGGTTTAATGGGTTCTGCTGCTTTAACATCATTC
    TTAACTGGTATTACAGAACCATTAGAATTCTCATTCTTATTTGTAGCACCATTATTATTC
    TTTATTCACGCAGTACTTGATGGTTTATCATTCTTAACATTGTACTTATTAGATCTTCAT
    CTAGGTTATACATTCTCAGGTGGTTTCATCGACTACTTCTTACTCGGTATACTACCTAAT
    AAGACACAATGGTGGTTAGTCATTCCTGTAGGTCTTGTATACGCAGTTATTTACTACTTC
    GTATTCCGATTCTTAATTGTAAAATTAAAATACAAAACACCAGGTCGTGAAGATAAACAA
    TCACAAGCGGCTACTGCTTCAGCAACTGAATTACCATATGCAGTATTAGAAGCTATGGGT
    GGCAAAGCAAACATTAAACATTTAGACGCTTGTATCACACGTCTACGTGTTGAAGTTAAC
    GACAAATCTAAAGTTGATGTTCCTGGTTTGAAAGATTTAGGCGCATCTGGTGTATTAGAA
    GTCGGCAATAATATGCAAGCAATTTTTGGTCCTAAATCTGACCAAATCAAACATGAAATG
    CAACAGATTATGAATGGTCAAGTAGTAGAAAATCCTACTACTATGGAAGACGATAAAGAC
    GAAACTGTTGTTGTTGCAGAAGATAAATCTGCAACAAGCGAATTGAGCCATATCGTGCAT
    GCACCATTAACTGGTGAAGTAACACCATTATCAGAAGTGCCTGATCAAGTGTTCAGCGAA
    AAAATGATGGGTGACGGTATCGCTATCAAACCTTCACAAGGTGAAGTTCGTGCACCATTC
    AACGGTAAAGTACAAATGATTTTCCCAACAAAACATGCAATTGGTCTTGTATCAGATAGT
    GGTTTAGAACTATTAATCCACATCGGTTTAGACACTGTTAAATTAAACGGAGAAGGCTTT
    ACTTTACATGTTGAGGAAGGTCAAGAAGTTAAACAAGGTGATTTATTAATCAACTTTGAT
    TTAGACTACATCCGCAATCATGCAAAGAGTGATATTACGCCTATTATCGTGACACAAGGA
    AACATTACAAACCTTGATTTTAAACAAGGTGAACATGGCAACATTTCATTTGGCGATCAA
    TTATTTGAAGCTAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1740
    1800
    1860
    1920
    1980
    1998

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SAOUHSC_00155
  • symbol: SAOUHSC_00155
  • description: PTS system glucose-specific protein
  • length: 665
  • theoretical pI: 5.7965
  • theoretical MW: 72055.3
  • GRAVY: 0.336241

Function[edit source | edit]

  • reaction:
    EC 2.7.1.69?  ExPASy
    Transferred entry: 2.7.1.191
    EC 2.7.1.199?  ExPASy
    Protein-N(pi)-phosphohistidine--D-glucose phosphotransferase[Protein]-N(pi)-phospho-L-histidine + D-glucose(Side 1) = [protein]-L-histidine + D-glucose 6-phosphate(Side 2)
  • TIGRFAM:
    MetabolismTransport and binding proteinsCarbohydrates, organic alcohols, and acidsPTS system, glucose-specific IIBC component (TIGR02002; EC 2.7.1.69; HMM-score: 771.6)
    MetabolismTransport and binding proteinsCarbohydrates, organic alcohols, and acidsPTS system, N-acetylglucosamine-specific IIBC component (TIGR01998; EC 2.7.1.69; HMM-score: 460.5)
    MetabolismTransport and binding proteinsCarbohydrates, organic alcohols, and acidsPTS system, maltose and glucose-specific IIBC component (TIGR02004; EC 2.7.1.69; HMM-score: 385.3)
    MetabolismTransport and binding proteinsCarbohydrates, organic alcohols, and acidsPTS system, maltose and glucose-specific subfamily, IIC component (TIGR00852; HMM-score: 279.3)
    Signal transductionSignal transductionPTSPTS system, maltose and glucose-specific subfamily, IIC component (TIGR00852; HMM-score: 279.3)
    MetabolismTransport and binding proteinsCarbohydrates, organic alcohols, and acidsPTS system, alpha-glucoside-specific IIBC component (TIGR02005; EC 2.7.1.69; HMM-score: 271)
    MetabolismTransport and binding proteinsCarbohydrates, organic alcohols, and acidsPTS system, IIBC component (TIGR02003; EC 2.7.1.69; HMM-score: 230.2)
    PTS system, beta-glucoside-specific IIABC component (TIGR01995; EC 2.7.1.69; HMM-score: 209.8)
    MetabolismTransport and binding proteinsCarbohydrates, organic alcohols, and acidsPTS system, glucose subfamily, IIA component (TIGR00830; HMM-score: 165.5)
    Signal transductionSignal transductionPTSPTS system, glucose subfamily, IIA component (TIGR00830; HMM-score: 165.5)
    MetabolismTransport and binding proteinsCarbohydrates, organic alcohols, and acidsPTS system, glucose-like IIB component (TIGR00826; EC 2.7.1.69; HMM-score: 73.8)
    Signal transductionSignal transductionPTSPTS system, glucose-like IIB component (TIGR00826; EC 2.7.1.69; HMM-score: 73.8)
    PTS system, sucrose-specific IIBC component (TIGR01996; EC 2.7.1.69; HMM-score: 55.9)
    PTS system, trehalose-specific IIBC component (TIGR01992; EC 2.7.1.69; HMM-score: 28.5)
    Genetic information processingProtein fateProtein and peptide secretion and traffickingtype I secretion membrane fusion protein, HlyD family (TIGR01843; HMM-score: 16.6)
    MetabolismTransport and binding proteinsUnknown substrateefflux transporter, RND family, MFP subunit (TIGR01730; HMM-score: 14.3)
  • TheSEED:  
    Trehalose Uptake and Utilization PTS system, glucose-specific IIC component / PTS system, glucose-specific IIB component (EC 2.7.1.69) / PTS system, glucose-specific IIA component 
  • PFAM:
    PTS_EIIC (CL0493) PTS_EIIC; Phosphotransferase system, EIIC (PF02378; HMM-score: 322.9)
    Hybrid (CL0105) PTS_EIIA_1; phosphoenolpyruvate-dependent sugar phosphotransferase system, EIIA 1 (PF00358; HMM-score: 181.7)
    no clan definedPTS_EIIB; phosphotransferase system, EIIB (PF00367; HMM-score: 53.1)
    Hybrid (CL0105) Biotin_lipoyl_2; Biotin-lipoyl like (PF13533; HMM-score: 20.2)
    no clan definedPIG-P; PIG-P (PF08510; HMM-score: 15)
    Hybrid (CL0105) Peptidase_M23; Peptidase family M23 (PF01551; HMM-score: 12.3)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 10
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular Possibility: 0
    • Signal Peptide Possibility: 0
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.242
    • Ymax_pos: 22
    • Cmax: 0.184
    • Cmax_pos: 22
    • Smax: 0.622
    • Smax_pos: 3
    • Smean: 0.374
    • D: 0.293
  • predicted transmembrane helices (TMHMM): 10

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MLPVAILPAAGLLLAIGTAMQGESLQHYLPFIQNGGVQTVAKLMTGAGGIIFDNLPMIFALGVAIGLAGGDGVAAIAAFVGYIIMNKTMGDFLQVTPKNIGDPASGYASILGIPTLQTGVFGGIIIGALAAWCYNKFYNINLPSYLGFFAGKRFVPIMMATTSFILAFPMALIWPTIQSGLNAFSTGLLDSNTGVAVFLFGFIKRLLIPFGLHHIFHAPFWFEFGSWKNAAGEIIHGDQRIFIEQIREGAHLTAGKFMQGEFPVMMFGLPAAALAIYHTAKPENKKVVAGLMGSAALTSFLTGITEPLEFSFLFVAPLLFFIHAVLDGLSFLTLYLLDLHLGYTFSGGFIDYFLLGILPNKTQWWLVIPVGLVYAVIYYFVFRFLIVKLKYKTPGREDKQSQAATASATELPYAVLEAMGGKANIKHLDACITRLRVEVNDKSKVDVPGLKDLGASGVLEVGNNMQAIFGPKSDQIKHEMQQIMNGQVVENPTTMEDDKDETVVVAEDKSATSELSHIVHAPLTGEVTPLSEVPDQVFSEKMMGDGIAIKPSQGEVRAPFNGKVQMIFPTKHAIGLVSDSGLELLIHIGLDTVKLNGEGFTLHVEEGQEVKQGDLLINFDLDYIRNHAKSDITPIIVTQGNITNLDFKQGEHGNISFGDQLFEAK

Experimental data[edit source | edit]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

  • SAOUHSC_00155 no polycistronic organisation predicted

Regulation[edit source | edit]

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-3661
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  2. Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet.: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

Relevant publications[edit source | edit]