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NCBI: 03-AUG-2016

Summary[edit source | edit]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_00153
  • pan locus tag?: SAUPAN001030000
  • symbol: SAOUHSC_00153
  • pan gene symbol?:
  • synonym:
  • product: indolepyruvate decarboxylase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SAOUHSC_00153
  • symbol: SAOUHSC_00153
  • product: indolepyruvate decarboxylase
  • replicon: chromosome
  • strand: -
  • coordinates: 164749..166389
  • length: 1641
  • essential: no DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    ATGAAACAACGCATTGGAGCTTACTTAATTGACGCTATTCATCGAGCAGGCGTCGATAAA
    ATTTTTGGTGTTCCTGGTGATTTTAATCTCGCTTTTCTAGACGATATTATCAGCAATCCC
    AATGTAGATTGGGTAGGAAATACAAATGAATTAAACGCAAGTTACGCAGCGGACGGTTAT
    GCCCGTCTTAATGGACTCGCTGCATTAGTTACTACATTTGGTGTTGGCGAATTAAGTGCC
    GTCAACGGTATCGCAGGTTCATATGCTGAACGCATACCTGTCATTGCGATTACAGGTGCG
    CCGACACGTGCTGTTGAACAAGGCGGTAAATATGTACATCACTCACTTGGTGAAGGTACA
    TTTGACGACTATCGAAAAATGTTTGCACATATAACCGTTGCACAAGGTTATATCACACCT
    GAAAATGCAACAACCGAAATACCACGTTTAATTAATACAGCAATCGCCGAAAGACGCCCA
    GTTCATTTACATTTACCAATCGATGTCGCAATCTCTGAAATTGAGATACCGACACCATTT
    GAAGTGACGGCAACTAAATATACGGATGCATCAACATATATAGAGTTATTAGCAACTAAA
    CTGCATCAAGCGAAGCAGCCTATCATCATTACTGGACATGAAATTAACAGTTTTCACCTC
    CATCAAGAATTAGAAGATTTTGTAAATCAAACACAGATACCAGTAGCACAACTTTCATTA
    GGAAAAGGTGCTTTTAATGAGGAAAATCCATATTATATGGGTATTTACGATGGGAAAATT
    GCCGAAGATAAAATACGAGATTATGTGGACAACAGCGATTTAATTTTAAATATTGGAGCC
    AAATTAACAGATTCAGCAACAGCAGGTTTTTCATACCAATTCAATATCGATGATGTCGTT
    ATGTTAAATCATCACAATATCAAAATTGACGATGTTACAAATGATGAAATATCTCTACCA
    TCATTGTTAAAACAGTTATCCAATATTTCATATACGAATAACGCAACGTTCCCTGCGTAT
    CATCGTCCAACATCACCCGATTATACTGTTGGCACAGAACCATTAACACAACAAACTTAT
    TTTAAAATGATGCAAAATTTCTTAAAACCAAATGATGTCATCATTGCTGATCAAGGTACA
    TCATTCTTTGGTGCTTATGATTTAGCATTATACAAAAACAATACTTTTATAGGGCAACCG
    TTATGGGGTTCTATCGGCTATACATTACCTGCAACATTAGGTTCACAATTAGCAGACAAA
    GATCGTCGTAACTTATTATTAATTGGTGATGGCTCATTGCAACTAACTGTTCAAGCTATT
    TCAACTATGATTAGACAGCATATTAAACCGGTATTATTTGTGATTAATAATGACGGCTAT
    ACGGTAGAACGACTTATTCACGGCATGTATGAACCTTATAATGAAATTCACATGTGGGAT
    TATAAAGCTTTACCAGCTGTATTTGGTGGTAAAAATGTTGAAATTCATGACGTTGAATCA
    TCAAAAGATTTACAAGACACGTTTAATGCAATTAATGGTCATCCCGATGTGATGCATTTT
    GTCGAAGTCAAAATGGCTGTCGAAGACGCACCGAAGAAACTCATCGATATCGCTAAAGCT
    TTTTCACAACAAAATAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1641

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SAOUHSC_00153
  • symbol: SAOUHSC_00153
  • description: indolepyruvate decarboxylase
  • length: 546
  • theoretical pI: 5.0203
  • theoretical MW: 60538
  • GRAVY: -0.171429

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    MetabolismCentral intermediary metabolismOtherindolepyruvate decarboxylase (TIGR03393; EC 4.1.1.74; HMM-score: 528.9)
    indolepyruvate/phenylpyruvate decarboxylase (TIGR03394; EC 4.1.1.43,4.1.1.74; HMM-score: 197.2)
    MetabolismAmino acid biosynthesisPyruvate familyacetolactate synthase, large subunit, biosynthetic type (TIGR00118; EC 2.2.1.6; HMM-score: 159.9)
    MetabolismCentral intermediary metabolismOthersulfoacetaldehyde acetyltransferase (TIGR03457; EC 2.3.3.15; HMM-score: 99.9)
    MetabolismEnergy metabolismSugars3,5/4-trihydroxycyclohexa-1,2-dione hydrolase (TIGR04377; EC 3.7.1.-; HMM-score: 88.8)
    glyoxylate carboligase (TIGR01504; EC 4.1.1.47; HMM-score: 78.8)
    MetabolismEnergy metabolismFermentationacetolactate synthase, catabolic (TIGR02418; EC 2.2.1.6; HMM-score: 77.1)
    MetabolismEnergy metabolismAerobicpyruvate oxidase (TIGR02720; EC 1.2.3.3; HMM-score: 63.3)
    Cellular processesCellular processesDetoxificationoxalyl-CoA decarboxylase (TIGR03254; EC 4.1.1.8; HMM-score: 49.2)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersMenaquinone and ubiquinone2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylic-acid synthase (TIGR00173; EC 2.2.1.9; HMM-score: 41.9)
    sulfopyruvate decarboxylase, beta subunit (TIGR03846; EC 4.1.1.79; HMM-score: 18.8)
    MetabolismEnergy metabolismChemoautotrophyCO dehydrogenase/acetyl-CoA synthase complex, epsilon subunit (TIGR00315; EC 1.2.99.2; HMM-score: 13.9)
  • TheSEED:  
    CarbohydratesCentral carbohydrate metabolismPyruvate metabolism II: acetyl-CoA, acetogenesis from pyruvate Pyruvate decarboxylase (EC 4.1.1.1) 
    Pyruvate metabolism II: acetyl-CoA, acetogenesis from pyruvate Alpha-keto-acid decarboxylase (EC 4.1.1.-) 
  • PFAM:
    THDP-binding (CL0254) TPP_enzyme_N; Thiamine pyrophosphate enzyme, N-terminal TPP binding domain (PF02776; HMM-score: 118)
    FAD_DHS (CL0085) TPP_enzyme_M; Thiamine pyrophosphate enzyme, central domain (PF00205; HMM-score: 84.7)
    THDP-binding (CL0254) TPP_enzyme_C; Thiamine pyrophosphate enzyme, C-terminal TPP binding domain (PF02775; HMM-score: 59.7)
    FAD_DHS (CL0085) CO_dh; CO dehydrogenase beta subunit/acetyl-CoA synthase epsilon subunit (PF02552; HMM-score: 14.2)

Structure, modifications & interactions[edit source | edit]

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.67
    • Cytoplasmic Membrane Score: 0.01
    • Cellwall Score: 0.15
    • Extracellular Score: 0.17
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.113
    • Ymax_pos: 31
    • Cmax: 0.139
    • Cmax_pos: 57
    • Smax: 0.155
    • Smax_pos: 30
    • Smean: 0.107
    • D: 0.111
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MKQRIGAYLIDAIHRAGVDKIFGVPGDFNLAFLDDIISNPNVDWVGNTNELNASYAADGYARLNGLAALVTTFGVGELSAVNGIAGSYAERIPVIAITGAPTRAVEQGGKYVHHSLGEGTFDDYRKMFAHITVAQGYITPENATTEIPRLINTAIAERRPVHLHLPIDVAISEIEIPTPFEVTATKYTDASTYIELLATKLHQAKQPIIITGHEINSFHLHQELEDFVNQTQIPVAQLSLGKGAFNEENPYYMGIYDGKIAEDKIRDYVDNSDLILNIGAKLTDSATAGFSYQFNIDDVVMLNHHNIKIDDVTNDEISLPSLLKQLSNISYTNNATFPAYHRPTSPDYTVGTEPLTQQTYFKMMQNFLKPNDVIIADQGTSFFGAYDLALYKNNTFIGQPLWGSIGYTLPATLGSQLADKDRRNLLLIGDGSLQLTVQAISTMIRQHIKPVLFVINNDGYTVERLIHGMYEPYNEIHMWDYKALPAVFGGKNVEIHDVESSKDLQDTFNAINGHPDVMHFVEVKMAVEDAPKKLIDIAKAFSQQNK

Experimental data[edit source | edit]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. 1.00 1.01 1.02 1.03 1.04 1.05 1.06 1.07 1.08 1.09 1.10 1.11 1.12 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J. Proteome Res.: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)
  2. Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  3. Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-61
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  4. Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet.: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

Relevant publications[edit source | edit]