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NCBI: 03-AUG-2016

Summary[edit source | edit]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_02119
  • pan locus tag?: SAUPAN004929000
  • symbol: SAOUHSC_02119
  • pan gene symbol?: putP
  • synonym:
  • product: high affinity proline permease

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SAOUHSC_02119
  • symbol: SAOUHSC_02119
  • product: high affinity proline permease
  • replicon: chromosome
  • strand: +
  • coordinates: 1991873..1993411
  • length: 1539
  • essential: no DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    ATGCTTACAATGGGGACAGCATTAAGTCAACAAGTAGATGCCAATTGGCAAACTTATATT
    ATGATTGCCGTCTACTTCTTGATACTAATCGTTATTGGCTTTTACGGTTACAAGCAAGCA
    ACTGGTAACCTAAGCGAGTACATGTTAGGTGGACGTAGTATTGGACCGTATATTACTGCA
    TTATCAGCTGGAGCTTCAGATATGAGTGGATGGATGATTATGGGGCTACCTGGTTCTGTC
    TATAGCACTGGTCTATCAGCTATGTGGATTACAATCGGTTTAACATTAGGTGCTTATATA
    AATTACTTTGTTGTTGCTCCTAGACTTCGTGTTTATACCGAATTAGCTGGAGATGCAATT
    ACATTACCAGATTTCTTTAAAAATCGTTTAAACGATAAAAATAATGTGTTAAAGATTATT
    TCTGGATTGATTATCGTAGTATTCTTTACATTATATACACATTCTGGTTTCGTATCTGGT
    GGTAAACTATTTGAAAGTGCTTTTGGATTAGATTATCATTTCGGTTTAATATTAGTTGCT
    TTCATTGTCATTTTCTATACTTTCTTTGGTGGATATTTAGCTGTATCAATTACAGATTTC
    TTCCAAGGTGTCATTATGTTAATTGCGATGGTTATGGTCCCTATTGTTGCTATGATGAAT
    TTAAACGGCTGGGGAACGTTTCATGATGTAGCAGCTATGAAACCTACAAATTTAAATTTA
    TTTAAAGGGTTATCATTTATAGGAATTATCTCTCTATTTTCATGGGGATTAGGTTATTTC
    GGTCAACCTCATATCATTGTAAGGTTTATGTCTATTAAATCACACAAGATGCTACCTAAA
    GCTAGACGTTTAGGTATTAGCTGGATGGCTGTTGGTTTATTAGGCGCTGTGGCTGTTGGT
    TTAACAGGTATTGCATTCGTACCTGCTTATCATATTAAACTAGAAGATCCTGAGACATTA
    TTCATCGTGATGAGTCAAGTACTCTTCCATCCTCTTGTAGGTGGTTTCTTACTTGCTGCG
    ATTCTAGCTGCAATTATGAGCACGATTTCTTCACAATTACTTGTAACATCTAGTTCACTA
    ACGGAAGACTTTTATAAATTAATTCGTGGTGAAGAAAAAGCTAAAACGCACCAAAAAGAA
    TTTGTTATGATTGGAAGATTATCTGTATTAGTTGTAGCAATTGTTGCCATCGCGATTGCA
    TGGAATCCAAACGACACAATTCTAAACTTAGTAGGTAACGCTTGGGCCGGATTTGGTGCA
    TCGTTCAGTCCACTTGTGCTATTTGCACTTTACTGGAAAGGTTTGACACGTGCCGGTGCT
    GTAAGTGGAATGGTTTCAGGTGCCTTAGTCGTTATCGTTTGGATTGCATGGATTAAACCA
    TTGGCACATATCAACGAAATATTCGGCTTATATGAAATTATTCCTGGATTTATTGTAAGT
    GTAATCGTTACATATGTTGTAAGTAAACTTACTAAAAAACCTGGTGCATTTGTTGAAACT
    GACTTAAACAAAGTTCGTGACATCGTTAGAGAAAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1539

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SAOUHSC_02119
  • symbol: SAOUHSC_02119
  • description: high affinity proline permease
  • length: 512
  • theoretical pI: 9.68032
  • theoretical MW: 55975.1
  • GRAVY: 0.778711

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    MetabolismTransport and binding proteinsAmino acids, peptides and aminessodium/proline symporter (TIGR02121; HMM-score: 768.8)
    MetabolismTransport and binding proteinsCations and iron carrying compoundstransporter, solute:sodium symporter (SSS) family (TIGR00813; HMM-score: 297.2)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersPantothenate and coenzyme Asodium/pantothenate symporter (TIGR02119; HMM-score: 202.8)
    MetabolismTransport and binding proteinsOthersodium/pantothenate symporter (TIGR02119; HMM-score: 202.8)
    probable sodium:solute symporter, VC_2705 subfamily (TIGR03648; HMM-score: 121.4)
    MetabolismTransport and binding proteinsCarbohydrates, organic alcohols, and acidscation/acetate symporter ActP (TIGR02711; HMM-score: 107)
  • TheSEED:  
    Amino Acids and DerivativesProline and 4-hydroxyprolineProline, 4-hydroxyproline uptake and utilization Proline/sodium symporter PutP (TC 2.A.21.2.1) 
    Proline, 4-hydroxyproline uptake and utilization Propionate/sodium symporter 
  • PFAM:
    APC (CL0062) SSF; Sodium:solute symporter family (PF00474; HMM-score: 329)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 13
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular Possibility: 0.17
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.121
    • Ymax_pos: 17
    • Cmax: 0.182
    • Cmax_pos: 17
    • Smax: 0.132
    • Smax_pos: 1
    • Smean: 0.084
    • D: 0.107
  • predicted transmembrane helices (TMHMM): 13

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MLTMGTALSQQVDANWQTYIMIAVYFLILIVIGFYGYKQATGNLSEYMLGGRSIGPYITALSAGASDMSGWMIMGLPGSVYSTGLSAMWITIGLTLGAYINYFVVAPRLRVYTELAGDAITLPDFFKNRLNDKNNVLKIISGLIIVVFFTLYTHSGFVSGGKLFESAFGLDYHFGLILVAFIVIFYTFFGGYLAVSITDFFQGVIMLIAMVMVPIVAMMNLNGWGTFHDVAAMKPTNLNLFKGLSFIGIISLFSWGLGYFGQPHIIVRFMSIKSHKMLPKARRLGISWMAVGLLGAVAVGLTGIAFVPAYHIKLEDPETLFIVMSQVLFHPLVGGFLLAAILAAIMSTISSQLLVTSSSLTEDFYKLIRGEEKAKTHQKEFVMIGRLSVLVVAIVAIAIAWNPNDTILNLVGNAWAGFGASFSPLVLFALYWKGLTRAGAVSGMVSGALVVIVWIAWIKPLAHINEIFGLYEIIPGFIVSVIVTYVVSKLTKKPGAFVETDLNKVRDIVREK

Experimental data[edit source | edit]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

  • SAOUHSC_02119 no polycistronic organisation predicted

Regulation[edit source | edit]

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  2. Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-61
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  3. Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet.: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

Relevant publications[edit source | edit]

William R Schwan, Lynn Lehmann, James McCormick
Transcriptional activation of the Staphylococcus aureus putP gene by low-proline-high osmotic conditions and during infection of murine and human tissues.
Infect. Immun.: 2006, 74(1);399-409
[PubMed:16368996] [WorldCat.org] [DOI] (P p)
W R Schwan, S N Coulter, E Y Ng, M H Langhorne, H D Ritchie, L L Brody, S Westbrock-Wadman, A S Bayer, K R Folger, C K Stover
Identification and characterization of the PutP proline permease that contributes to in vivo survival of Staphylococcus aureus in animal models.
Infect. Immun.: 1998, 66(2);567-72
[PubMed:9453610] [WorldCat.org] (P p)
A S Bayer, S N Coulter, C K Stover, W R Schwan
Impact of the high-affinity proline permease gene (putP) on the virulence of Staphylococcus aureus in experimental endocarditis.
Infect. Immun.: 1999, 67(2);740-4
[PubMed:9916085] [WorldCat.org] (P p)