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NCBI: 06-JUL-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_1840 [new locus tag: NWMN_RS10575 ]
  • pan locus tag?: SAUPAN004929000
  • symbol: putP
  • pan gene symbol?: putP
  • synonym:
  • product: high affinity proline permease

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: NWMN_1840 [new locus tag: NWMN_RS10575 ]
  • symbol: putP
  • product: high affinity proline permease
  • replicon: chromosome
  • strand: +
  • coordinates: 2048186..2049715
  • length: 1530
  • essential: unknown other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    ATGGGGACAGCATTAAGTCAACAAGTAGATGCCAATTGGCAAACTTATATTATGATTGCC
    GTCTACTTCTTGATACTAATCGTTATTGGCTTTTACGGTTACAAGCAAGCAACTGGTAAC
    CTAAGCGAGTACATGTTAGGTGGACGTAGTATTGGACCGTATATTACTGCATTATCAGCT
    GGAGCTTCAGATATGAGTGGATGGATGATTATGGAGCTACCTGGTTCTGTCTATAGCACT
    GGTCTATCAGCTATGTGGATTACAATCGGTTTAACATTAGGTGCTTATATAAATTACTTT
    GTTGTTGCTCCTAGACTTCGTGTTTATACCGAATTAGCTGGAGATGCAATTACATTACCA
    GATTTCTTTAAAAATCGTTTAAACGATAAAAATAATGTGTTAAAGATTATTTCTGGATTG
    ATTATCGTAGTATTCTTTACATTATATACACATTCTGGTTTCGTATCTGGTGGTAAACTA
    TTTGAAAGTGCTTTTGGATTAGATTATCATTTCGGTTTAATATTAGTTGCTTTCATTGTC
    ATTTTCTATACTTTCTTTGGTGGATATTTAGCTGTATCAATTACAGATTTCTTCCAAGGT
    GTCATTATGTTAATTGCGATGGTTATGGTCCCTATTGTTGCTATGATGAATTTAAACGGC
    TGGGGAACGTTTCATGATGTAGCAGCTATGAAACCTACAAATTTAAATTTATTTAAAGGG
    TTATCATTTATAGGAATTATCTCTCTATTTTCATGGGGATTAGGTTATTTCGGTCAACCT
    CATATCATTGTAAGGTTTATGTCTATTAAATCACACAAGATGCTACCTAAAGCTAGACGT
    TTAGGTATTAGCTGGATGGCTGTTGGTTTATTAGGCGCTGTGGCTGTTGGTTTAACAGGT
    ATTGCATTCGTACCTGCTTATCATATTAAACTAGAAGATCCTGAGACATTATTCATCGTG
    ATGAGTCAAGTACTCTTCCATCCTCTTGTAGGTGGTTTCTTACTTGCTGCGATTCTAGCT
    GCAATTATGAGCACGATTTCTTCACAATTACTTGTAACATCTAGTTCACTAACGGAAGAC
    TTTTATAAATTAATTCGTGGTGAAGAAAAAGCTAAAACGCACCAAAAAGAATTTGTTATG
    ATTGGAAGATTATCTGTATTAGTTGTAGCAATTGTTGCCATCGCGATTGCATGGAATCCA
    AACGACACAATTCTAAACTTAGTAGGTAACGCTTGGGCCGGATTTGGTGCATCGTTCAGT
    CCACTTGTGCTATTTGCACTTTACTGGAAAGGTTTGACACGTGCCGGTGCTGTAAGTGGA
    ATGGTTTCAGGTGCCTTAGTCGTTATCGTTTGGATTGCGTGGATTAAACCATTGGCACAT
    ATCAACGAAATATTCGGCTTATATGAAATTATTCCTGGATTTATTGTAAGTGTAATCGTT
    ACATATGTTGTAAGTAAACTTACTAAAAAACCTGGTGCATTTGTTGAAACTGACTTAAAC
    AAAGTTCGTGACATCGTTAGAGAAAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1530

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: NWMN_1840 [new locus tag: NWMN_RS10575 ]
  • symbol: PutP
  • description: high affinity proline permease
  • length: 509
  • theoretical pI: 9.59755
  • theoretical MW: 55701.7
  • GRAVY: 0.767387

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    MetabolismTransport and binding proteinsAmino acids, peptides and aminessodium/proline symporter (TIGR02121; HMM-score: 765)
    MetabolismTransport and binding proteinsCations and iron carrying compoundstransporter, solute:sodium symporter (SSS) family (TIGR00813; HMM-score: 292.5)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersPantothenate and coenzyme Asodium/pantothenate symporter (TIGR02119; HMM-score: 201)
    MetabolismTransport and binding proteinsOthersodium/pantothenate symporter (TIGR02119; HMM-score: 201)
    probable sodium:solute symporter, VC_2705 subfamily (TIGR03648; HMM-score: 120)
    MetabolismTransport and binding proteinsCarbohydrates, organic alcohols, and acidscation/acetate symporter ActP (TIGR02711; HMM-score: 104.5)
  • TheSEED:  
    Amino Acids and DerivativesProline and 4-hydroxyprolineProline, 4-hydroxyproline uptake and utilization Proline/sodium symporter PutP (TC 2.A.21.2.1) 
    Proline, 4-hydroxyproline uptake and utilization Propionate/sodium symporter 
  • PFAM:
    APC (CL0062) SSF; Sodium:solute symporter family (PF00474; HMM-score: 323.7)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 12
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular Possibility: 0.17
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.108
    • Ymax_pos: 38
    • Cmax: 0.162
    • Cmax_pos: 38
    • Smax: 0.105
    • Smax_pos: 33
    • Smean: 0.054
    • D: 0.087
  • predicted transmembrane helices (TMHMM): 13

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MGTALSQQVDANWQTYIMIAVYFLILIVIGFYGYKQATGNLSEYMLGGRSIGPYITALSAGASDMSGWMIMELPGSVYSTGLSAMWITIGLTLGAYINYFVVAPRLRVYTELAGDAITLPDFFKNRLNDKNNVLKIISGLIIVVFFTLYTHSGFVSGGKLFESAFGLDYHFGLILVAFIVIFYTFFGGYLAVSITDFFQGVIMLIAMVMVPIVAMMNLNGWGTFHDVAAMKPTNLNLFKGLSFIGIISLFSWGLGYFGQPHIIVRFMSIKSHKMLPKARRLGISWMAVGLLGAVAVGLTGIAFVPAYHIKLEDPETLFIVMSQVLFHPLVGGFLLAAILAAIMSTISSQLLVTSSSLTEDFYKLIRGEEKAKTHQKEFVMIGRLSVLVVAIVAIAIAWNPNDTILNLVGNAWAGFGASFSPLVLFALYWKGLTRAGAVSGMVSGALVVIVWIAWIKPLAHINEIFGLYEIIPGFIVSVIVTYVVSKLTKKPGAFVETDLNKVRDIVREK

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

  • putP no polycistronic organisation predicted

Regulation[edit source | edit]

  • sigma factor:
  • regulator: CcpA
    CcpA (TF) important in Carbon catabolismRegPrecise

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]