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NCBI: 03-AUG-2016

Summary[edit source | edit]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_01818
  • pan locus tag?: SAUPAN004361000
  • symbol: SAOUHSC_01818
  • pan gene symbol?: ald2
  • synonym:
  • product: alanine dehydrogenase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SAOUHSC_01818
  • symbol: SAOUHSC_01818
  • product: alanine dehydrogenase
  • replicon: chromosome
  • strand: -
  • coordinates: 1723533..1724651
  • length: 1119
  • essential: no DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    ATGAAAATTGGTATACCAAGGGAGATTAAAAATAATGAAAATCGTGTTGGTTTATCACCA
    AGTGGTGTGCACGCTTTAGTAGAAAGTGGGCATACTGTTTTAGTGGAAACAAATGCGGGT
    TCAGGATCATTCTTTGAAGATGTAGATTACAAAGAAGCAGGTGCTGAGATTGTTGCTGAA
    CAAGCAAAAGTTTGGGATGTGGATATGGTTATTAAAGTTAAAGAACCACTTGAATCTGAA
    TATCCATATTTTAAAGAAGGGCTTGTATTATTCACTTATCTTCATTTAGCAAATGAAGAA
    AAATTAACACAAGCTTTGATAGATAGAAAAGTAATTAGTATTGCATATGAGACTGTGCAG
    TTACCAGACCGATCTTTACCATTGTTATCACCAATGAGTGAGGTAGCAGGAAGAATGTCA
    GCTCAAGTTGGCGCAGAGTTCCTACAAAAACTTAATGGTGGTATGGGAATTCTACTAGGT
    GGTGTCCCAGGAGTACCTAAGGGTAAAGTAACTATTATCGGTGGTGGTCAAGCAGGAACA
    AATGCAGCTAAAATTGCACTAGGACTAGGTGCAGATGTTACGATTTTAGATGTTAATCCA
    AAGCGTTTACAACAATTAGATGATTTATTCGGTGGACGTGTACATACAATTATGTCAAAT
    CCGTTGAATATTGAGTTGTATGTTAAACAAAGTGATTTAGTAATAGGTGCAGTTTTAATT
    CCAGGTGCTAAAGCGCCAAGACTTGTAACAGAAGACATGATTAAACAAATGAAAAATGGG
    TCAGTTATTATTGACATTGCTATTGATCAAGGCGGTATTTTTGAAACAACTGATAAAATT
    ACGACACATGATGATCCTACATATATTAAGCATGGTGTGGTTCATTATGCAGTTGCAAAT
    ATGCCAGGTGCAGTACCGCGTACTTCGACGTTAGCTTTAAATAATGCTACGCTACCTTAT
    GCGCTCATGCTAGCTAATAAAGGGTATAGAGAAGCATTTAAATCAAATCAACCATTATCA
    TTAGGTTTAAATACTTACAAAGGTCACGTAACCAATAAAGGCGTTGCAGAGGCATTTGAA
    ATGGAATATAAATCTGTAGAAGAAGCATTACAATTATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1119

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SAOUHSC_01818
  • symbol: SAOUHSC_01818
  • description: alanine dehydrogenase
  • length: 372
  • theoretical pI: 5.6562
  • theoretical MW: 40104.9
  • GRAVY: -0.0215054

Function[edit source | edit]

  • reaction:
    EC 1.4.1.1?  ExPASy
    Alanine dehydrogenaseL-alanine + H2O + NAD+ = pyruvate + NH3 + NADH
  • TIGRFAM:
    MetabolismEnergy metabolismAmino acids and aminesalanine dehydrogenase (TIGR00518; EC 1.4.1.1; HMM-score: 575.8)
    MetabolismEnergy metabolismElectron transportNAD(P)(+) transhydrogenase (AB-specific), alpha subunit (TIGR00561; EC 1.6.1.2; HMM-score: 151.3)
    Cellular processesCellular processesSporulation and germinationdipicolinic acid synthetase, A subunit (TIGR02853; HMM-score: 26.8)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersHeme, porphyrin, and cobalaminglutamyl-tRNA reductase (TIGR01035; EC 1.2.1.70; HMM-score: 19.3)
    dihydrolipoyl dehydrogenase (TIGR01350; EC 1.8.1.4; HMM-score: 18.6)
    mycofactocin system FadH/OYE family oxidoreductase 2 (TIGR03997; EC 1.-.-.-; HMM-score: 16)
    MetabolismEnergy metabolismElectron transportglutathione-disulfide reductase (TIGR01424; EC 1.8.1.7; HMM-score: 15.8)
    MetabolismEnergy metabolismAmino acids and aminesadenosylhomocysteinase (TIGR00936; EC 3.3.1.1; HMM-score: 13.8)
    MetabolismPurines, pyrimidines, nucleosides, and nucleotidesPurine ribonucleotide biosynthesisphosphoribosylaminoimidazole carboxylase, ATPase subunit (TIGR01161; EC 4.1.1.21; HMM-score: 13.3)
    6-hydroxycyclohex-1-ene-1-carbonyl-CoA dehydrogenase (TIGR03201; EC 1.1.1.-; HMM-score: 13.3)
    nucleotide sugar dehydrogenase (TIGR03026; HMM-score: 12.6)
    Unknown functionEnzymes of unknown specificityflavoprotein, HI0933 family (TIGR00275; HMM-score: 12.5)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersChlorophyll and bacteriochlorphyllgeranylgeranyl reductase family (TIGR02032; EC 1.3.1.-; HMM-score: 12.4)
    mycothione reductase (TIGR03452; EC 1.8.1.15; HMM-score: 12.3)
    Cell structureCell envelopeBiosynthesis and degradation of surface polysaccharides and lipopolysaccharidessurface carbohydrate biosynthesis protein (TIGR04396; HMM-score: 11.5)
    Genetic information processingProtein synthesistRNA and rRNA base modificationtRNA uridine 5-carboxymethylaminomethyl modification enzyme GidA (TIGR00136; HMM-score: 11.3)
  • TheSEED:  
    CarbohydratesCentral carbohydrate metabolismPyruvate Alanine Serine Interconversions Alanine dehydrogenase (EC 1.4.1.1) 
  • PFAM:
    NADP_Rossmann (CL0063) AlaDh_PNT_C; Alanine dehydrogenase/PNT, C-terminal domain (PF01262; HMM-score: 284.6)
    Form_Glyc_dh (CL0325) AlaDh_PNT_N; Alanine dehydrogenase/PNT, N-terminal domain (PF05222; HMM-score: 155.2)
    NADP_Rossmann (CL0063) 2-Hacid_dh_C; D-isomer specific 2-hydroxyacid dehydrogenase, NAD binding domain (PF02826; HMM-score: 30.3)
    Shikimate_DH; Shikimate / quinate 5-dehydrogenase (PF01488; HMM-score: 26.5)
    Pyr_redox; Pyridine nucleotide-disulphide oxidoreductase (PF00070; HMM-score: 26.5)
    Pyr_redox_2; Pyridine nucleotide-disulphide oxidoreductase (PF07992; HMM-score: 21.1)
    3HCDH_N; 3-hydroxyacyl-CoA dehydrogenase, NAD binding domain (PF02737; HMM-score: 18.5)
    F420_oxidored; NADP oxidoreductase coenzyme F420-dependent (PF03807; HMM-score: 18.3)
    NAD_binding_2; NAD binding domain of 6-phosphogluconate dehydrogenase (PF03446; HMM-score: 17.1)
    GIDA; Glucose inhibited division protein A (PF01134; HMM-score: 16.9)
    TrkA_N; TrkA-N domain (PF02254; HMM-score: 16.8)
    THF_DHG_CYH_C; Tetrahydrofolate dehydrogenase/cyclohydrolase, NAD(P)-binding domain (PF02882; HMM-score: 16.6)
    NAD_binding_7; Putative NAD(P)-binding (PF13241; HMM-score: 14.1)
    HI0933_like; HI0933-like protein (PF03486; HMM-score: 13.7)
    AdoHcyase_NAD; S-adenosyl-L-homocysteine hydrolase, NAD binding domain (PF00670; HMM-score: 13.1)
    UDPG_MGDP_dh_N; UDP-glucose/GDP-mannose dehydrogenase family, NAD binding domain (PF03721; HMM-score: 12.6)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.144
    • Ymax_pos: 26
    • Cmax: 0.13
    • Cmax_pos: 26
    • Smax: 0.217
    • Smax_pos: 32
    • Smean: 0.162
    • D: 0.151
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MKIGIPREIKNNENRVGLSPSGVHALVESGHTVLVETNAGSGSFFEDVDYKEAGAEIVAEQAKVWDVDMVIKVKEPLESEYPYFKEGLVLFTYLHLANEEKLTQALIDRKVISIAYETVQLPDRSLPLLSPMSEVAGRMSAQVGAEFLQKLNGGMGILLGGVPGVPKGKVTIIGGGQAGTNAAKIALGLGADVTILDVNPKRLQQLDDLFGGRVHTIMSNPLNIELYVKQSDLVIGAVLIPGAKAPRLVTEDMIKQMKNGSVIIDIAIDQGGIFETTDKITTHDDPTYIKHGVVHYAVANMPGAVPRTSTLALNNATLPYALMLANKGYREAFKSNQPLSLGLNTYKGHVTNKGVAEAFEMEYKSVEEALQL

Experimental data[edit source | edit]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  2. Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-3661
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  3. Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet.: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

Relevant publications[edit source | edit]