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NCBI: 06-JUL-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_1630 [new locus tag: NWMN_RS09155 ]
  • pan locus tag?: SAUPAN004402000
  • symbol: NWMN_1630
  • pan gene symbol?: aroA2
  • synonym:
  • product: bifunctional 3-deoxy-7-phosphoheptulonate synthase/chorismate mutase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: NWMN_1630 [new locus tag: NWMN_RS09155 ]
  • symbol: NWMN_1630
  • product: bifunctional 3-deoxy-7-phosphoheptulonate synthase/chorismate mutase
  • replicon: chromosome
  • strand: -
  • coordinates: 1814675..1815766
  • length: 1092
  • essential: unknown other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    ATGAGTAATAAATTAGAATCATACAGAAGTGAGATTGTATCACTGAATCATCAAATTTTA
    GACTTATTATCTAAACGTGGTGAACTAGCACAAAAAATTGGGGAAGAAAAATTAAAACAA
    GGTACACGTATTTATGATCCACAACGTGAAAAAGAAATGCTTAACGACTTAATCGATAGT
    AACAAAGGACCATTCAACGATAATACTATTAAGCAATTATTTAAAGAAATTTTCAAAGCC
    TCTACAGATTTACAAAAATCTGAAAATGAAAAACATTTATATGTATCACGTAAGTTGAAA
    CCTGAAGATACGATTGTAACATTTGATAATGGGGGCATTATTGGAGACGGCAATAAATCA
    TTTGTATTTGGGCCATGTTCAGTTGAATCATTTGAACAAGTTGAAGCTGTTGCTAAAAAC
    TTACATGCTAAAGGTGAAAAATTTATTCGTGGCGGTGCATTTAAACCACGTACATCACCA
    TATGATTTCCAAGGCCTAGGTGTTGAAGGACTTAAAATACTTAAACAGATTAAAGATAAA
    TATGATTTAAATGTTGTCAGCGAAATCGTAAATCCAAATGATTTTGAAGTGGCTGATGAG
    TATTTAGACGTATTCCAAATTGGTGCACGTAATATGCAAAACTTCGAGTTATTAAAAGAA
    GCTGGCCGTACGAAAAAGCCTATTCTATTAAAACGTGGTTTATCTGCTACAATCGAAGAG
    TTTGTTTATGCAGCTGAATACATTGCTTCACAAGGTAATCAAAACATTATTTTATGTGAA
    CGTGGAATCCGAACTTATGAAAAGGCGACACGTAACACTTTAGATATTTCAGCAGTACCA
    ATTTTAAAACAAGGTACACACTTACCAGTCATGGTAGATGTTACGCATAGTACAGGTCGT
    AAAGATATCATGTTACCAACTGCGAAAGCAGCATTAGCAGTTGGTGCTGATGGAGTTATG
    GCTGAGGTGCATCCAGATCCATCTGTTGCACTTAGTGATGCGGGTCAACAAATGGATTTA
    GATGAATTCCAAGCATTTTATGATGAATTAAAGCCTTTAGCTGATTTATATAACGCTAAA
    AAGTTAAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1092

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: NWMN_1630 [new locus tag: NWMN_RS09155 ]
  • symbol: NWMN_1630
  • description: bifunctional 3-deoxy-7-phosphoheptulonate synthase/chorismate mutase
  • length: 363
  • theoretical pI: 6.02863
  • theoretical MW: 40618
  • GRAVY: -0.44022

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    MetabolismAmino acid biosynthesisAromatic amino acid family3-deoxy-7-phosphoheptulonate synthase (TIGR01361; EC 2.5.1.54; HMM-score: 381.7)
    MetabolismAmino acid biosynthesisAromatic amino acid familychorismate mutase (TIGR01801; EC 5.4.99.5; HMM-score: 170.2)
    Cell structureCell envelopeBiosynthesis and degradation of surface polysaccharides and lipopolysaccharides3-deoxy-8-phosphooctulonate synthase (TIGR01362; EC 2.5.1.55; HMM-score: 121.6)
    MetabolismAmino acid biosynthesisAromatic amino acid familychorismate mutase (TIGR01807; EC 5.4.99.5; HMM-score: 64.4)
    MetabolismAmino acid biosynthesisAromatic amino acid familychorismate mutase (TIGR01791; EC 5.4.99.5; HMM-score: 53.5)
    MetabolismAmino acid biosynthesisAromatic amino acid familychorismate mutase (TIGR01805; EC 5.4.99.5; HMM-score: 45.9)
    MetabolismAmino acid biosynthesisAromatic amino acid familychorismate mutase (TIGR01797; EC 5.4.99.5; HMM-score: 36.2)
    MetabolismAmino acid biosynthesisAromatic amino acid familychorismate mutase (TIGR01799; EC 5.4.99.5; HMM-score: 36.2)
    MetabolismAmino acid biosynthesisAromatic amino acid familychorismate mutase (TIGR01808; EC 5.4.99.5; HMM-score: 35.4)
    pseudaminic acid synthase (TIGR03586; EC 2.5.1.97; HMM-score: 28.5)
    MetabolismAmino acid biosynthesisAromatic amino acid familyputative chorismate mutase (TIGR01806; EC 5.4.99.5; HMM-score: 28.3)
    N-acetylneuraminate synthase (TIGR03569; EC 2.5.1.56; HMM-score: 18.3)
  • TheSEED:  
    Chorismate Synthesis Chorismate mutase I (EC 5.4.99.5) / 2-keto-3-deoxy-D-arabino-heptulosonate-7-phosphate synthase I beta (EC 2.5.1.54) 
    Common Pathway For Synthesis of Aromatic Compounds (DAHP synthase to chorismate) Chorismate mutase I (EC 5.4.99.5) / 2-keto-3-deoxy-D-arabino-heptulosonate-7-phosphate synthase I beta (EC 2.5.1.54) 
    Phenylalanine and Tyrosine Branches from Chorismate Chorismate mutase I (EC 5.4.99.5) / 2-keto-3-deoxy-D-arabino-heptulosonate-7-phosphate synthase I beta (EC 2.5.1.54) 
  • PFAM:
    TIM_barrel (CL0036) DAHP_synth_1; DAHP synthetase I family (PF00793; HMM-score: 287.3)
    no clan definedCM_2; Chorismate mutase type II (PF01817; HMM-score: 74.1)
    TIM_barrel (CL0036) NeuB; NeuB family (PF03102; HMM-score: 32.7)
    no clan definedSPOB_ab; Sporulation initiation phospho-transferase B, C-terminal (PF14682; HMM-score: 15.7)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.134
    • Ymax_pos: 33
    • Cmax: 0.121
    • Cmax_pos: 33
    • Smax: 0.235
    • Smax_pos: 1
    • Smean: 0.125
    • D: 0.131
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MSNKLESYRSEIVSLNHQILDLLSKRGELAQKIGEEKLKQGTRIYDPQREKEMLNDLIDSNKGPFNDNTIKQLFKEIFKASTDLQKSENEKHLYVSRKLKPEDTIVTFDNGGIIGDGNKSFVFGPCSVESFEQVEAVAKNLHAKGEKFIRGGAFKPRTSPYDFQGLGVEGLKILKQIKDKYDLNVVSEIVNPNDFEVADEYLDVFQIGARNMQNFELLKEAGRTKKPILLKRGLSATIEEFVYAAEYIASQGNQNIILCERGIRTYEKATRNTLDISAVPILKQGTHLPVMVDVTHSTGRKDIMLPTAKAALAVGADGVMAEVHPDPSVALSDAGQQMDLDEFQAFYDELKPLADLYNAKKLK

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]