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NCBI: 06-JUL-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_1663 [new locus tag: NWMN_RS09335 ]
  • pan locus tag?: SAUPAN004460000
  • symbol: NWMN_1663
  • pan gene symbol?:
  • synonym:
  • product: hypothetical protein

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: NWMN_1663 [new locus tag: NWMN_RS09335 ]
  • symbol: NWMN_1663
  • product: hypothetical protein
  • replicon: chromosome
  • strand: -
  • coordinates: 1859282..1860784
  • length: 1503
  • essential: unknown other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    ATGCGTGTAGCAATAATAGGCATGGGAACTGCTGGTGTAAGTGTATTGAGAGAGTTAGTT
    AAGCACCCGAAGTTTAATCAATTAGATATAGATTTATACGATGATAAAGTAAATATGGGA
    CAGGGCGTACCATTTCAAAATGATAGTTCTGAGCTGCTAATTAATATGCCTTCGAAAAAG
    ATGAGTTTAAATTTAGATGATGAGACTGAATTTTGGAAGTGGTATAAACAACAAACTGAT
    TTTAATTTTGATGAACCTGCATATTTACCGAGATTTGTATTCGGACATTATATGAAATCT
    TATTTATCTATGTTCACGAAAAAATATCCAAATATATCAACTAATTATAATAAAGTTCAA
    GAGATTTATACAAATTCTAATATCGATGAAACGAATTTAACTTATTACATATGTACTACA
    AATTCAGGACAATCATGGCAAGCGTACGATTATGTATTTTTAACATGTGGTACATTTGCC
    TACCATGATCCATATAACTTAAAGGGTAAAAAAGGATATATCGCTACGCCTTATCCAACA
    TATAATACGTTAGACGAAGTAAATGAACTGGACGATATTGCGATTATTGGTACAGGCCTT
    GCCAGTTTAGATGTTGTCCGTTATGTTGCTGCTCATCATCCAAAATTACCAATTACTATG
    ACGAGTCGTTCTGCTCATTTACCAAGTGTAAGAGGTACAATGATAGACGTGACATTCAAG
    TACTTAACTAAAGACAAATTAAATGACATTAAAAAACATCATTTCGGCAATGCACCGCTT
    GATACTTTAGTTTCTTTATTTTTAAAAGAATGTGCTGATTATGACATTGATTTTAAAAAA
    TTAGTACATAGACGTACAGGAAATCATATTGCAGATTTGAAGTATGATTTAGCGCGTCCA
    ACAGAAATGGGCATTTTCCAAAGTATGATTGAACATTTGAAAGAGAATTTAAATTGGATA
    TGGAATAGCTTAAGTATTGAAGACCAACATCAATTTAATCAAAAATACTCAAAGATGATT
    CAATTAAATTCAAATCCAATGCCTCCAAGAACTGCTGAACTAATTATTGAATTAATAGAA
    AATAAGTCTTTAATTTTGAAAAAAGATTTAGAAGATGTGAAACATGATGGCAAGTTGTAT
    TATTTTTCATACAAAAACCAAGAATCAGTAGACATATATAATGTTGTCATTAACGCGACA
    GGTGCAAAAAGTCATTTAAATGAATTGGATCAAGATGATCAATTAATTAGAAATCTTGAA
    AATAGACAAATTGTACAAGCACACCCAATGGGAGGCATACAAATCATTCCGGAAACCAAT
    CAAGTTATTAGTCCAAGGTTTGGAACATTAACAAATATGATTGCGATAGGTCAAATGACA
    AACGGTGTTAATAAACTACGAAACGGTGTGAAAATGATTGTAGAACAAGTTGCGCATACA
    GTATCTCAATTATATGATGCTTTAGAATCGAATGAGCAACAACAGCGCAGTGACAATCAA
    TAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1503

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: NWMN_1663 [new locus tag: NWMN_RS09335 ]
  • symbol: NWMN_1663
  • description: hypothetical protein
  • length: 500
  • theoretical pI: 6.41197
  • theoretical MW: 57563
  • GRAVY: -0.475

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    Genetic information processingProtein synthesistRNA and rRNA base modificationtRNA U-34 5-methylaminomethyl-2-thiouridine biosynthesis protein MnmC, C-terminal domain (TIGR03197; HMM-score: 15.4)
    salicylate 1-monooxygenase (TIGR03219; EC 1.14.13.1; HMM-score: 14.9)
  • TheSEED:  
    COG4529 
  • PFAM:
    NADP_Rossmann (CL0063) NAD_binding_9; FAD-NAD(P)-binding (PF13454; HMM-score: 113.8)
    Pyr_redox_2; Pyridine nucleotide-disulphide oxidoreductase (PF07992; HMM-score: 32.6)
    Pyr_redox_3; Pyridine nucleotide-disulphide oxidoreductase (PF13738; HMM-score: 24.8)
    K_oxygenase; L-lysine 6-monooxygenase (NADPH-requiring) (PF13434; HMM-score: 20.1)
    DAO; FAD dependent oxidoreductase (PF01266; HMM-score: 17)
    Thi4; Thi4 family (PF01946; HMM-score: 15)
    Sacchrp_dh_NADP; Saccharopine dehydrogenase NADP binding domain (PF03435; HMM-score: 13)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 2.5
    • Cytoplasmic Membrane Score: 2.5
    • Cellwall Score: 2.5
    • Extracellular Score: 2.5
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 0.83
    • Signal Peptide Possibility: -0.5
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.167
    • Ymax_pos: 21
    • Cmax: 0.108
    • Cmax_pos: 29
    • Smax: 0.337
    • Smax_pos: 1
    • Smean: 0.265
    • D: 0.205
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MRVAIIGMGTAGVSVLRELVKHPKFNQLDIDLYDDKVNMGQGVPFQNDSSELLINMPSKKMSLNLDDETEFWKWYKQQTDFNFDEPAYLPRFVFGHYMKSYLSMFTKKYPNISTNYNKVQEIYTNSNIDETNLTYYICTTNSGQSWQAYDYVFLTCGTFAYHDPYNLKGKKGYIATPYPTYNTLDEVNELDDIAIIGTGLASLDVVRYVAAHHPKLPITMTSRSAHLPSVRGTMIDVTFKYLTKDKLNDIKKHHFGNAPLDTLVSLFLKECADYDIDFKKLVHRRTGNHIADLKYDLARPTEMGIFQSMIEHLKENLNWIWNSLSIEDQHQFNQKYSKMIQLNSNPMPPRTAELIIELIENKSLILKKDLEDVKHDGKLYYFSYKNQESVDIYNVVINATGAKSHLNELDQDDQLIRNLENRQIVQAHPMGGIQIIPETNQVISPRFGTLTNMIAIGQMTNGVNKLRNGVKMIVEQVAHTVSQLYDALESNEQQQRSDNQ

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor: SigB [1] other strains
    SigB (sigma factor) controls a large regulon involved in stress/starvation response and adaptation
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Markus Bischoff, Paul Dunman, Jan Kormanec, Daphne Macapagal, Ellen Murphy, William Mounts, Brigitte Berger-Bächi, Steven Projan
    Microarray-based analysis of the Staphylococcus aureus sigmaB regulon.
    J. Bacteriol.: 2004, 186(13);4085-99
    [PubMed:15205410] [WorldCat.org] [DOI] (P p)

Relevant publications[edit source | edit]